Table 5
(continued)
Enzyme Source
Transduction
a
Analyte Immobilization/electrode
Electron
transfer
Detection
limit
Linear range
Sample
References
Nitroreductase E.
cloacae
Voltammetric (CV,
−0.35 V)
TNT
PPB electropolymerization/NR-MBP
adsorption/GC
Mediated
(PPB)
2
µM
u pt o4 0µM
Buffer
[218]
Cytochrome c
reductase
porcine heart
Voltammetric (CV,
−0.45 V)
Cyt c
PPy electrodeposition/drop cast
Nafion + ethanol + CNTs/CcR + glutaraldehyde
cross-linking/CSPE
Direct ET
10 nM
10 nM–500
µM
Cardiomyocytes [219]
a
All potentials are quoted vs. Ag/AgCl reference electrodes. BMIMBF4
1-n-butyl-3-methylimidazolium tetrafluoroborate, BSA
bovine serum albumin, CcR
cytochrome c
reductase, CSPE
carbon screen-printed electrode, CV
cyclic voltammetry, Cyt
cytochrome; DMSO
dimethyl sulfoxide, ET
electron transfer, G
graphite, GC
glassy carbon, GNP
gold nanoparticles, GSH
glutathione, GSSG
glutathione disulfide, MV
methyl viologen, MWCNT
multi-walled carbon nanotubes, NaR
nitrate reductase, NiR
nitrite reductase, NOR
nitric oxide reductase, NR-MBP
nitroreductase-maltose binding protein, PG
pyrolytic graphite, polyTTBA
poly[2,2′:5,2″-terthiophene-30-(p-benzoic acid)], PPB
N-(3-pyrrol-1-ylpropyl)-4,4’-bipyridine, PPy
polypyrrole, PVA
poly(vinyl alcohol), SWCNT
singled-walled carbon nanotubes, SWV
square wave voltammetry, TMAO
trimethylamine N-oxide, TNT
trinitrotoluene
334
T. Monteiro et al.
(continued)
Enzyme Source
Transduction
a
Analyte Immobilization/electrode
Electron
transfer
Detection
limit
Linear range
Sample
References
Nitroreductase E.
cloacae
Voltammetric (CV,
−0.35 V)
TNT
PPB electropolymerization/NR-MBP
adsorption/GC
Mediated
(PPB)
2
µM
u pt o4 0µM
Buffer
[218]
Cytochrome c
reductase
porcine heart
Voltammetric (CV,
−0.45 V)
Cyt c
PPy electrodeposition/drop cast
Nafion + ethanol + CNTs/CcR + glutaraldehyde
cross-linking/CSPE
Direct ET
10 nM
10 nM–500
µM
Cardiomyocytes [219]
a
All potentials are quoted vs. Ag/AgCl reference electrodes. BMIMBF4
1-n-butyl-3-methylimidazolium tetrafluoroborate, BSA
bovine serum albumin, CcR
cytochrome c
reductase, CSPE
carbon screen-printed electrode, CV
cyclic voltammetry, Cyt
cytochrome; DMSO
dimethyl sulfoxide, ET
electron transfer, G
graphite, GC
glassy carbon, GNP
gold nanoparticles, GSH
glutathione, GSSG
glutathione disulfide, MV
methyl viologen, MWCNT
multi-walled carbon nanotubes, NaR
nitrate reductase, NiR
nitrite reductase, NOR
nitric oxide reductase, NR-MBP
nitroreductase-maltose binding protein, PG
pyrolytic graphite, polyTTBA
poly[2,2′:5,2″-terthiophene-30-(p-benzoic acid)], PPB
N-(3-pyrrol-1-ylpropyl)-4,4’-bipyridine, PPy
polypyrrole, PVA
poly(vinyl alcohol), SWCNT
singled-walled carbon nanotubes, SWV
square wave voltammetry, TMAO
trimethylamine N-oxide, TNT
trinitrotoluene
334
T. Monteiro et al.