85. Xiu Z-L, Zeng A-P (2008) Present state and perspective of downstream processing of
biologically produced 1,3-propanediol and 2,3-butanediol. Appl Microbiol Biotechnol
78:917–926
86. Heap JT, Kuehne SA, Ehsaan M, Cartman ST, Cooksley CM, Scott JC, Minton NP (2010)
The ClosTron: Mutagenesis in Clostridium refined and streamlined. J Microbiol Methods
80:49–55
87. Köpke M, Gerth ML, Maddock DJ, Müller AP, Liew F, Simpson SD, Patrick WM (2014)
Reconstruction of an acetogenic 2,3-butanediol pathway involving a novel
NADPH-dependent primary-secondary alcohol dehydrogenase. Appl Environ Microbiol
80:3394–3403
88. Liew F, Henstra AM, Köpke M, Winzer K, Simpson SD, Minton NP (2017) Metabolic
engineering of Clostridium autoethanogenum for selective alcohol production. Metab Eng
40:104–114
89. Phillips JR, Atiyeh HK, Tanner RS, Torres JR, Saxena J, Wilkins MR, Huhnke RL (2015)
Butanol and hexanol production in Clostridium carboxidivorans syngas fermentation:
Medium development and culture techniques. Bioresour Technol 190:114–121
90. Liou JS, Balkwill DL, Drake GR, Tanner RS (2005) Clostridium carboxidivorans sp. nov., a
solvent-producing Clostridium isolated from an agricultural settling lagoon, and reclassification of the acetogen Clostridium scatologenes strain SL1 as Clostridium drakei sp. nov.
Int J Syst Evol Microbiol 55:2085–2091
91. Shen GJ, Shieh JS, Grethlein AJ, Jain MK, Zeikus JG (1999) Biochemical basis for carbon
monoxide tolerance and butanol production by Butyribacterium methylotrophicum. Appl
Microbiol Biotechnol 51:827–832
92. Maddipati P, Atiyeh HK, Bellmer DD, Huhnke RL (2011) Ethanol production from syngas
by Clostridium strain P11 using corn steep liquor as a nutrient replacement to yeast extract.
Bioresour Technol 102:6494–6501
93. Chang IS, Kim BH, Kim DH, Lovitt RW, Sung HC (1999) Formulation of defined media for
carbon monoxide fermentation by Eubacterium limosum KIST612 and the growth
characteristics of the bacterium. J Biosci Bioeng 88:682–685
94. Dürre P (2016) Butanol formation from gaseous substrates. FEMS Microbiol Lett 363
95. Li F, Hinderberger J, Seedorf H, Zhang J, Buckel W, Thauer RK (2008) Coupled ferredoxin
and crotonyl coenzyme A (CoA) reduction with NADH catalyzed by the butyryl-CoA
dehydrogenase/Etf complex from Clostridium kluyveri. J Bacteriol 190:843–850
96. Jeong J, Bertsch J, Hess V, Choi S, Choi IG, Chang IS, Müller V (2015) Energy
conservation model based on genomic and experimental analyses of a carbon
monoxide-utilizing, butyrate-forming acetogen, Eubacterium limosum KIST612. Appl
Environ Microbiol 81:4782–4790
97. Fernández Naveira Á, Veiga M, Kennes C (2017) H-B-E (Hexanol-Butanol-Ethanol)
fermentation for the production of higher alcohols from syngas/waste gas. J Chem Technol
Biotechnol 92:712–731
98. Ramió-Pujol S, Ganigué R, Bañeras L, Colprim J (2015) Incubation at 25 °C prevents acid
crash and enhances alcohol production in Clostridium carboxidivorans P7. Bioresour
Technol 192:296–303
99. Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP (2007) The ClosTron: a
universal gene knock-out system for the genus Clostridium. J Microbiol Methods 70:452–
464
100. Papoutsakis ET (2008) Engineering solventogenic clostridia. Curr Opin Biotechnol 19:420–
429
101. Strätz M, Sauer U, Kuhn A, Dürre P (1994) Plasmid transfer into the homoacetogen
Acetobacterium woodii by electroporation and conjugation. Appl Environ Microbiol
60:1033–1037
102. Schiel-Bengelsdorf B, Dürre P (2012) Pathway engineering and synthetic biology using
acetogens. FEBS Lett 586:2191–2198
Acetogenic Bacteria for Biotechnological Applications
129
biologically produced 1,3-propanediol and 2,3-butanediol. Appl Microbiol Biotechnol
78:917–926
86. Heap JT, Kuehne SA, Ehsaan M, Cartman ST, Cooksley CM, Scott JC, Minton NP (2010)
The ClosTron: Mutagenesis in Clostridium refined and streamlined. J Microbiol Methods
80:49–55
87. Köpke M, Gerth ML, Maddock DJ, Müller AP, Liew F, Simpson SD, Patrick WM (2014)
Reconstruction of an acetogenic 2,3-butanediol pathway involving a novel
NADPH-dependent primary-secondary alcohol dehydrogenase. Appl Environ Microbiol
80:3394–3403
88. Liew F, Henstra AM, Köpke M, Winzer K, Simpson SD, Minton NP (2017) Metabolic
engineering of Clostridium autoethanogenum for selective alcohol production. Metab Eng
40:104–114
89. Phillips JR, Atiyeh HK, Tanner RS, Torres JR, Saxena J, Wilkins MR, Huhnke RL (2015)
Butanol and hexanol production in Clostridium carboxidivorans syngas fermentation:
Medium development and culture techniques. Bioresour Technol 190:114–121
90. Liou JS, Balkwill DL, Drake GR, Tanner RS (2005) Clostridium carboxidivorans sp. nov., a
solvent-producing Clostridium isolated from an agricultural settling lagoon, and reclassification of the acetogen Clostridium scatologenes strain SL1 as Clostridium drakei sp. nov.
Int J Syst Evol Microbiol 55:2085–2091
91. Shen GJ, Shieh JS, Grethlein AJ, Jain MK, Zeikus JG (1999) Biochemical basis for carbon
monoxide tolerance and butanol production by Butyribacterium methylotrophicum. Appl
Microbiol Biotechnol 51:827–832
92. Maddipati P, Atiyeh HK, Bellmer DD, Huhnke RL (2011) Ethanol production from syngas
by Clostridium strain P11 using corn steep liquor as a nutrient replacement to yeast extract.
Bioresour Technol 102:6494–6501
93. Chang IS, Kim BH, Kim DH, Lovitt RW, Sung HC (1999) Formulation of defined media for
carbon monoxide fermentation by Eubacterium limosum KIST612 and the growth
characteristics of the bacterium. J Biosci Bioeng 88:682–685
94. Dürre P (2016) Butanol formation from gaseous substrates. FEMS Microbiol Lett 363
95. Li F, Hinderberger J, Seedorf H, Zhang J, Buckel W, Thauer RK (2008) Coupled ferredoxin
and crotonyl coenzyme A (CoA) reduction with NADH catalyzed by the butyryl-CoA
dehydrogenase/Etf complex from Clostridium kluyveri. J Bacteriol 190:843–850
96. Jeong J, Bertsch J, Hess V, Choi S, Choi IG, Chang IS, Müller V (2015) Energy
conservation model based on genomic and experimental analyses of a carbon
monoxide-utilizing, butyrate-forming acetogen, Eubacterium limosum KIST612. Appl
Environ Microbiol 81:4782–4790
97. Fernández Naveira Á, Veiga M, Kennes C (2017) H-B-E (Hexanol-Butanol-Ethanol)
fermentation for the production of higher alcohols from syngas/waste gas. J Chem Technol
Biotechnol 92:712–731
98. Ramió-Pujol S, Ganigué R, Bañeras L, Colprim J (2015) Incubation at 25 °C prevents acid
crash and enhances alcohol production in Clostridium carboxidivorans P7. Bioresour
Technol 192:296–303
99. Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP (2007) The ClosTron: a
universal gene knock-out system for the genus Clostridium. J Microbiol Methods 70:452–
464
100. Papoutsakis ET (2008) Engineering solventogenic clostridia. Curr Opin Biotechnol 19:420–
429
101. Strätz M, Sauer U, Kuhn A, Dürre P (1994) Plasmid transfer into the homoacetogen
Acetobacterium woodii by electroporation and conjugation. Appl Environ Microbiol
60:1033–1037
102. Schiel-Bengelsdorf B, Dürre P (2012) Pathway engineering and synthetic biology using
acetogens. FEBS Lett 586:2191–2198
Acetogenic Bacteria for Biotechnological Applications
129