4. Repeat steps 2 and 3 until the desired volume has been administered (up to 25 μL total), interchanging the nares between
administrations.
Fig. 5 Sublingual administration. Tongue positioning to achieve correctly
sublingual administration. With the animal anesthetized, place it on a flat
surface on its abdomen. Raise the animal’s head and, with the help of a
forceps, pick the tongue and lift it leaving the sublingual region exposed and
ready for administration
Fig. 6 Gavage needle sizing. Procedure to measure and choose the correct
gavage needle size. With the animal restrained, place the base of the gavage
needle near the mouth of the animal with the tip of the needle pointing to its
abdomen. The correct needle size must reach the xyphoid cartilage, located at
the bottom of the animal’s thorax
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