3.11 Intragastric
Administration
(Gavage)
1. Restrain the animal as described above.
2. Choose the right size of the gavage needle by measuring the
distance between the mouth and the xyphoid cartilage (Fig. 6).
3. Insert the gavage needle on the animal’s mouth beside the
bottom teeth and above the tongue (Fig. 7).
4. Push the animal’s head back with the needle so that the animal’s nose is facing up and the path to the stomach is straighter.
5. Continue to pass the needle until the measured distance is
reached.
6. Administrate the dose (maximum of 500 μL).
3.12 Intranasal
Administration
1. Restrain the animal as described above.
2. With the help of a micropipette, place a small volume (i.e. 5 μL)
of the dose on the nares of the animal to be inhaled (Fig. 8).
3. Wait until the dose disappears into the nares.
Fig. 4 Mice sedation for intraperitoneal anesthetic injection. With the mouse
restrained, visually divide the animal’s abdomen into four quadrants. Apply the
injection in the lower right (animal’s right side) quadrant in an approximately 45
angle
Lactic Acid Bacteria for Therapeutic Applications
455
Administration
(Gavage)
1. Restrain the animal as described above.
2. Choose the right size of the gavage needle by measuring the
distance between the mouth and the xyphoid cartilage (Fig. 6).
3. Insert the gavage needle on the animal’s mouth beside the
bottom teeth and above the tongue (Fig. 7).
4. Push the animal’s head back with the needle so that the animal’s nose is facing up and the path to the stomach is straighter.
5. Continue to pass the needle until the measured distance is
reached.
6. Administrate the dose (maximum of 500 μL).
3.12 Intranasal
Administration
1. Restrain the animal as described above.
2. With the help of a micropipette, place a small volume (i.e. 5 μL)
of the dose on the nares of the animal to be inhaled (Fig. 8).
3. Wait until the dose disappears into the nares.
Fig. 4 Mice sedation for intraperitoneal anesthetic injection. With the mouse
restrained, visually divide the animal’s abdomen into four quadrants. Apply the
injection in the lower right (animal’s right side) quadrant in an approximately 45
angle
Lactic Acid Bacteria for Therapeutic Applications
455