values. Taking into account that a RoboColumn run can be typically completed within a day (the method in Fig. 5 had a duration of
<8) with no end-user intervention, other than setting up the robot
and the method, demonstrates the power of the RoboColumn
technique as a tool for generating valuable process information in
an efficient and effective fashion. The benefit of adopting this highthroughput method is further compounded by the fact that viral
antigen products are characterized by even larger screening spaces
due to the existence of multiple strains and/or serotypes of viruses
(i.e., influenza, lentivirus, HPV). Here, the offered parallelization
and walk-away automation, can lead to a rapid development of
purification processes, which would be impossible to achieve with
conventional workflows, and more importantly to assess the potential of establishing a platform process for all different strains of a
given virus. This would allow for significant process development
simplification and return significant time and cost savings.
4 Notes
1. We use two MP3 pos carriers from Tecan making it possible to
store up to six plates on the robot’s deck. It is possible to use
two MP4 pos carriers allowing the storage of up to eight plates
instead.
2. It is possible to place the Te-Shuttle carrier to the front of three
MP3 pos carriers resulting in space saving on the robot’s deck
in the case of EVO
® 150 or EVO
® 100 instruments. To implement this, the worktable will have to be edited in EVOware by
changing the two carrier definitions:
(a) Locate the MP3 pos carrier in EVOware’s
CARRIERS tab.
(b) Right click and select Edit.
(c) Make note of carrier definitions (take and save a screen
capture).
(d) Change the X and Y Dimensions to 1 and 1 mm.
(e) Change to Y Reference Offset to 100 mm.
(f) Add three carriers, for example, grid locations 2, 8, and
14 (leave 6 grids spacing between each carrier).
(g) Locate the Te-Shuttle carrier in EVOware’s
CARRIERS tab.
(h) Right click and select Edit.
(i) Make note of carrier definitions (take and save a screen
capture).
HTPD Purification of Viral Antigens
131
<8) with no end-user intervention, other than setting up the robot
and the method, demonstrates the power of the RoboColumn
technique as a tool for generating valuable process information in
an efficient and effective fashion. The benefit of adopting this highthroughput method is further compounded by the fact that viral
antigen products are characterized by even larger screening spaces
due to the existence of multiple strains and/or serotypes of viruses
(i.e., influenza, lentivirus, HPV). Here, the offered parallelization
and walk-away automation, can lead to a rapid development of
purification processes, which would be impossible to achieve with
conventional workflows, and more importantly to assess the potential of establishing a platform process for all different strains of a
given virus. This would allow for significant process development
simplification and return significant time and cost savings.
4 Notes
1. We use two MP3 pos carriers from Tecan making it possible to
store up to six plates on the robot’s deck. It is possible to use
two MP4 pos carriers allowing the storage of up to eight plates
instead.
2. It is possible to place the Te-Shuttle carrier to the front of three
MP3 pos carriers resulting in space saving on the robot’s deck
in the case of EVO
® 150 or EVO
® 100 instruments. To implement this, the worktable will have to be edited in EVOware by
changing the two carrier definitions:
(a) Locate the MP3 pos carrier in EVOware’s
CARRIERS tab.
(b) Right click and select Edit.
(c) Make note of carrier definitions (take and save a screen
capture).
(d) Change the X and Y Dimensions to 1 and 1 mm.
(e) Change to Y Reference Offset to 100 mm.
(f) Add three carriers, for example, grid locations 2, 8, and
14 (leave 6 grids spacing between each carrier).
(g) Locate the Te-Shuttle carrier in EVOware’s
CARRIERS tab.
(h) Right click and select Edit.
(i) Make note of carrier definitions (take and save a screen
capture).
HTPD Purification of Viral Antigens
131