(j) Change the X and Y Dimensions to 1 and 1 mm.
(k) Add carriers to grid location 3.
(l) Change carrier definitions to their original values.
3. If using MP4 pos carriers or MP3 pos carriers placed behind
the Te-Shuttle as opposed to next to it, beware that the liquid
handling will be compromised due to the physical dimensions
of the robot. Channel 8 of the LiHa will not be able to reach
the first row of a plate in the first site of an MP4 pos carrier and
Channel 1 of the LiHa will not be able to reach the last row of a
plate in the fourth site of a MP4 pos carrier. In this case, liquid
handling commands will need to take such limitations into
consideration.
4. Depending on the installed EVOware version, it may not be
possible to define variables for grid numbers in Transfer Labware commands. This is circumvented by joining two 9-site
Hotel Carriers to a single 18-site hotel carrier. In this carrier
the first nine sites will have exactly the same X-Offsets as in a
conventional 9-site Hotel carrier, whereas the next nine sites
will have increased X-Offsets by ~128 mm.
5. Labware transfers are trained via carrier definitions. All such
training is to be made using a single location as a reference
point and by using a single plate as a reference labware.
6. The sanitization protocol washes the tips with 10 mL in the
waste and cleaner using the Fast-Wash module. This is followed
with two cycles of aspirating and dispensing 900 μL of 0.5 M
NaOH to a single trough and using a custom liquid class.
Finally, the tips are then washed again with 10 mL in the
waste and cleaner using the Fast-Wash module and then with
1 mL in the waste and cleaner while not using the Fast-Wash
module so as to regenerate the air gap.
7. The custom liquid class used for the sanitization has an 8 mm
offset in the aspiration so as to submerge the tips further in the
sanitization solution.
8. The sanitization protocol is effective in cleaning the tips for
most crude and purified proteinaceous solutions. It needs to be
tested in a new laboratory for its effectiveness by liquid
handling a sample, applying the sanitization protocol, liquid
handling a buffer or DI water and testing the lastly liquid
handled solution for its content in contaminants via a sensitive
assay (e.g., ELISA).
9. The number of steps in a gradient will affect the total number
of collected fractions and prepared elution buffers and also the
volumes of the collected fractions. For example, a 10 CV gradient with 100 steps will result in steps with a size of 0.1 of a
CV. In the case of both 0.2 and 0.6 mL RoboColumns, such
132
Shaleem I. Jacob et al.
(k) Add carriers to grid location 3.
(l) Change carrier definitions to their original values.
3. If using MP4 pos carriers or MP3 pos carriers placed behind
the Te-Shuttle as opposed to next to it, beware that the liquid
handling will be compromised due to the physical dimensions
of the robot. Channel 8 of the LiHa will not be able to reach
the first row of a plate in the first site of an MP4 pos carrier and
Channel 1 of the LiHa will not be able to reach the last row of a
plate in the fourth site of a MP4 pos carrier. In this case, liquid
handling commands will need to take such limitations into
consideration.
4. Depending on the installed EVOware version, it may not be
possible to define variables for grid numbers in Transfer Labware commands. This is circumvented by joining two 9-site
Hotel Carriers to a single 18-site hotel carrier. In this carrier
the first nine sites will have exactly the same X-Offsets as in a
conventional 9-site Hotel carrier, whereas the next nine sites
will have increased X-Offsets by ~128 mm.
5. Labware transfers are trained via carrier definitions. All such
training is to be made using a single location as a reference
point and by using a single plate as a reference labware.
6. The sanitization protocol washes the tips with 10 mL in the
waste and cleaner using the Fast-Wash module. This is followed
with two cycles of aspirating and dispensing 900 μL of 0.5 M
NaOH to a single trough and using a custom liquid class.
Finally, the tips are then washed again with 10 mL in the
waste and cleaner using the Fast-Wash module and then with
1 mL in the waste and cleaner while not using the Fast-Wash
module so as to regenerate the air gap.
7. The custom liquid class used for the sanitization has an 8 mm
offset in the aspiration so as to submerge the tips further in the
sanitization solution.
8. The sanitization protocol is effective in cleaning the tips for
most crude and purified proteinaceous solutions. It needs to be
tested in a new laboratory for its effectiveness by liquid
handling a sample, applying the sanitization protocol, liquid
handling a buffer or DI water and testing the lastly liquid
handled solution for its content in contaminants via a sensitive
assay (e.g., ELISA).
9. The number of steps in a gradient will affect the total number
of collected fractions and prepared elution buffers and also the
volumes of the collected fractions. For example, a 10 CV gradient with 100 steps will result in steps with a size of 0.1 of a
CV. In the case of both 0.2 and 0.6 mL RoboColumns, such
132
Shaleem I. Jacob et al.