2 Multifunctional Magnetic Nanocomposites on the Base of Magnetite. . .
37
Fig. 2.1 Isotherms (a) and kinetics (b) of the CP adsorption on magnetite (1) and Fe3O4/HA
NC (2)
and radiobiology of the National Academy of Sciences of Ukraine, which is now
at the stage of putting into production. The idea of the mentioned new form lies
in the strategy of overcoming the resistance of malignant tumors to cisplatin via
pharmacological correction of endogenous iron metabolism which is ensured by
utilization of a ferriferous nanocomposite and cisplatin.
For the adsorption investigations of CP on the Fe 3 O 4 and Fe 3 O 4 /HA samples
were used (Fig. 2.1a, b).
It was found that the highest cytotoxic activity of the nanocomposite for the
MCF-7/CP resistant line is related to a more active accumulation of ferromagnetic
nanoparticles in the cells, due a high level of transferrin receptors and a disturbance
of the system of antioxidant protection of the resistant cells. It was shown that a
ferrofluid is able to cause in cells of the resistant line more pronounced cytomorphological changes and genotoxic effects compared to cells of the sensitive line.
In the case of therapy of rats of the Wistar line with an FF containing inoculated
Geren carcinoma resistant to cisplatin, a positive antitumor effect on tumors of the
strain resistant to cisplatin was found: the mean volume of tumors decreased from
20.2 ± 1.0 cm 3 in the control group to 12.1 ± 2.4 cm 3 in animals that were injected
with the nanocomposite; the percentage of slowdown of the tumor growth was 40%.
Antibodies Binding to the Fe 3 O 4 /H£/Ag Nanocomposites The formation on the
surface of modified magnetosensitive carriers of the regions with spacers for binding
monoclonal antibodies is one of the important stages of synthesis of biofunctionalized nanocomposites. The purpose of this research was to study the processes of
antibody immobilization on the surface of Fe 3 O 4 /H£ and Fe 3 O 4 /H£/Ag. It was
found that the shape of the obtained isotherms of immunoglobulin adsorption varies
between different buffer systems (Figs. 2.2 and 2.3; Table 2.1).
Taking into account a high adsorption (£ = 34–38 mg/g) of immunoglobulin, the
formation of a compact packing of adsorbed antibodies on the NC surface modified
by HA and silver can be assumed. The given results of experimental research [10]
37
Fig. 2.1 Isotherms (a) and kinetics (b) of the CP adsorption on magnetite (1) and Fe3O4/HA
NC (2)
and radiobiology of the National Academy of Sciences of Ukraine, which is now
at the stage of putting into production. The idea of the mentioned new form lies
in the strategy of overcoming the resistance of malignant tumors to cisplatin via
pharmacological correction of endogenous iron metabolism which is ensured by
utilization of a ferriferous nanocomposite and cisplatin.
For the adsorption investigations of CP on the Fe 3 O 4 and Fe 3 O 4 /HA samples
were used (Fig. 2.1a, b).
It was found that the highest cytotoxic activity of the nanocomposite for the
MCF-7/CP resistant line is related to a more active accumulation of ferromagnetic
nanoparticles in the cells, due a high level of transferrin receptors and a disturbance
of the system of antioxidant protection of the resistant cells. It was shown that a
ferrofluid is able to cause in cells of the resistant line more pronounced cytomorphological changes and genotoxic effects compared to cells of the sensitive line.
In the case of therapy of rats of the Wistar line with an FF containing inoculated
Geren carcinoma resistant to cisplatin, a positive antitumor effect on tumors of the
strain resistant to cisplatin was found: the mean volume of tumors decreased from
20.2 ± 1.0 cm 3 in the control group to 12.1 ± 2.4 cm 3 in animals that were injected
with the nanocomposite; the percentage of slowdown of the tumor growth was 40%.
Antibodies Binding to the Fe 3 O 4 /H£/Ag Nanocomposites The formation on the
surface of modified magnetosensitive carriers of the regions with spacers for binding
monoclonal antibodies is one of the important stages of synthesis of biofunctionalized nanocomposites. The purpose of this research was to study the processes of
antibody immobilization on the surface of Fe 3 O 4 /H£ and Fe 3 O 4 /H£/Ag. It was
found that the shape of the obtained isotherms of immunoglobulin adsorption varies
between different buffer systems (Figs. 2.2 and 2.3; Table 2.1).
Taking into account a high adsorption (£ = 34–38 mg/g) of immunoglobulin, the
formation of a compact packing of adsorbed antibodies on the NC surface modified
by HA and silver can be assumed. The given results of experimental research [10]