36
Ie. V. Pylypchuk et al.
The choice of single-domain magnetite as an input material for NC synthesis is
due to its unique magnetic properties, satisfactory biocompatibility and biodegradation, accumulated experience in the field of surface modification, possibilities to
control the movement of nanoparticles by the means of the external magnetic field,
use of the magnetic separation method at the stages of separation, and removal of
adsorbents. The special features of single-domain state of magnetic particles include
the homogeneity of magnetization at any values and directions of the ° field, the
possibility of an existence of domains not only in solid ferro- and ferrimagnetic
alloys and compounds but also in liquid media (suspensions and colloids).
The usage of HA as a coating for magnetosensitive carriers is due to its high
biocompatibility with a living organism, stability in biological media, adsorbent
activity of the surface, possibility to ensure the necessary chemical functionalization
of the carrier surface for further biofunctionalization.
This work gives a short overview of the research studies done at the Chuiko Institute of surface chemistry of the National Academy of Sciences of Ukraine aimed at
the development of the concept of creation of magnetosensitive NCs of a multilevel
hierarchical nano-architecture and a function of medico-biological nanorobots. The
data are provided in relation to the synthesis of magnetosensitive NCs of the corecell type on the basis of single-domain magnetite and hydroxyapatite, specificity of
immobilization on the surface of oncologic drugs of different mechanisms of action,
bioactivity checking, development of the methodology of creation of ferrofluids on
the basis of synthesized NCs, and determination of perspectivity of their application
in oncology.
Synthesis of Magnetite/Hydroxyapatite Nanocomposites Synthesis of the
hydroxyapatite coating on the surface of a highly dispersed magnetite was carried
out by solgel method, according to reaction: 10´a(NO 3 ) 2 + 6(NH 4 ) 2 HPO 4 + 8NH 3
+ 2° 2 ± → ´Ã 10 (PO 4 ) 6 (±°) 2 + 20NH 4 NO 3 .
XRD patterns confirmed the presence of a magnetite phase (Fe 3 O 4 , JCPDS №19629) and hydroxyapatite (´Ã 10 (PO 4 ) 6 (±°) 2 , JCPDS №74-0566).
Synthesis of Fe 3 O 4 /H£/Ag Nanocomposites The modification of Fe 3 O 4 /H£
nanocomposite by silver nanoparticles was made from AgNO 3 solution. The
quantity of silver input in the reaction mixture made 1% of the mass of
Fe 3 O 4 /Ca 10 (PO 4 ) 6 (OH) 2 sample. The reduction of silver ions was made by 0.005%
hydrazine hydrate on heating and stirring according to the reactions: 4Ag + +
N 2 H 4 + 4OH − → 4Ag ◦ + N 2 + 4H 2 O.
The presence of silver on the surface is confirmed by the XRD analysis. The
mean size of Ag nanoparticles calculated as per the Scherrer formula was ∼ 10 nm.
Cytotoxic Properties of Ferrofluids on the Basis of Cisplatin (CP) The synthesized nanostructures as part of a ferrofluid (FF) were used for development of a new
form of the oncology drug “Ferroplat” which was for the first time experimentally
substantiated at the R.E. Kavetsky Institute for experimental pathology, oncology,
Ie. V. Pylypchuk et al.
The choice of single-domain magnetite as an input material for NC synthesis is
due to its unique magnetic properties, satisfactory biocompatibility and biodegradation, accumulated experience in the field of surface modification, possibilities to
control the movement of nanoparticles by the means of the external magnetic field,
use of the magnetic separation method at the stages of separation, and removal of
adsorbents. The special features of single-domain state of magnetic particles include
the homogeneity of magnetization at any values and directions of the ° field, the
possibility of an existence of domains not only in solid ferro- and ferrimagnetic
alloys and compounds but also in liquid media (suspensions and colloids).
The usage of HA as a coating for magnetosensitive carriers is due to its high
biocompatibility with a living organism, stability in biological media, adsorbent
activity of the surface, possibility to ensure the necessary chemical functionalization
of the carrier surface for further biofunctionalization.
This work gives a short overview of the research studies done at the Chuiko Institute of surface chemistry of the National Academy of Sciences of Ukraine aimed at
the development of the concept of creation of magnetosensitive NCs of a multilevel
hierarchical nano-architecture and a function of medico-biological nanorobots. The
data are provided in relation to the synthesis of magnetosensitive NCs of the corecell type on the basis of single-domain magnetite and hydroxyapatite, specificity of
immobilization on the surface of oncologic drugs of different mechanisms of action,
bioactivity checking, development of the methodology of creation of ferrofluids on
the basis of synthesized NCs, and determination of perspectivity of their application
in oncology.
Synthesis of Magnetite/Hydroxyapatite Nanocomposites Synthesis of the
hydroxyapatite coating on the surface of a highly dispersed magnetite was carried
out by solgel method, according to reaction: 10´a(NO 3 ) 2 + 6(NH 4 ) 2 HPO 4 + 8NH 3
+ 2° 2 ± → ´Ã 10 (PO 4 ) 6 (±°) 2 + 20NH 4 NO 3 .
XRD patterns confirmed the presence of a magnetite phase (Fe 3 O 4 , JCPDS №19629) and hydroxyapatite (´Ã 10 (PO 4 ) 6 (±°) 2 , JCPDS №74-0566).
Synthesis of Fe 3 O 4 /H£/Ag Nanocomposites The modification of Fe 3 O 4 /H£
nanocomposite by silver nanoparticles was made from AgNO 3 solution. The
quantity of silver input in the reaction mixture made 1% of the mass of
Fe 3 O 4 /Ca 10 (PO 4 ) 6 (OH) 2 sample. The reduction of silver ions was made by 0.005%
hydrazine hydrate on heating and stirring according to the reactions: 4Ag + +
N 2 H 4 + 4OH − → 4Ag ◦ + N 2 + 4H 2 O.
The presence of silver on the surface is confirmed by the XRD analysis. The
mean size of Ag nanoparticles calculated as per the Scherrer formula was ∼ 10 nm.
Cytotoxic Properties of Ferrofluids on the Basis of Cisplatin (CP) The synthesized nanostructures as part of a ferrofluid (FF) were used for development of a new
form of the oncology drug “Ferroplat” which was for the first time experimentally
substantiated at the R.E. Kavetsky Institute for experimental pathology, oncology,