139
Automatic Single-Cell Transfer Module
TABLE 7.3
Fibroblast Cell Detection Ratios for Different
Flow Speeds
Flow speed (in microchannel)
0.39 μL/min
Number of cells
55
Number of detected cells/ratio
55/100%
Flow speed (in microchannel)
0.50 μL/min
Number of cells
53
Number of detected cells/ratio
48/90.6%
Flow speed (in microchannel)
0.60 μL/min
Number of cells
46
Number of detected cells/ratio
34/74%
Flow speed (in microchannel)
0.65 μL/min
Number of cells
50
Number of detected cells/ratio
22/44%
Flow speed (in microchannel)
0.80 μL/min
Number of cells
50
Number of detected cells/ratio
0/0%
© 2009 IEEE.
calculated delay used in order to put a specific distance between each cell)
the suction mouth returns to the bottom surface and moves to the next cell,
numbered 1.
This procedure is repeated until the last cell is vacuumed from the screen
view. If the program reaches the total number of desired cells, it stops searching, moves to a safe height, and continues with the suction, even if there are
still some cells on the screen. The aspirated cells are batched in the docking area with a specific distance. The time interval between two connected
cells is given in Figure 7.9b for suction mode and supply mode. Although the
approximate absorption time for one cell to reach node A was found based
on the speed of the rotary pump and tube dimensions, the experimental
elapsed time values were different (Figure 7.11). The delay occurred due to
the drag resistance of cells as they move through the continuous fluid flow
in the Teflon tube.
7.3.2 Direction Control
Once aspiration of the desired number of cells is completed, the camera system on the cell container is toggled to the compact vision system placed on
the cross section of the “Y”-character microchannels (see Figure 7.11). The
task of the compact system is to ensure that the collected cells are gathered at
the dock and transported to the next module, by switching the valves. When
a new cell shows up in the upper right-hand corner, the program detects cells
Automatic Single-Cell Transfer Module
TABLE 7.3
Fibroblast Cell Detection Ratios for Different
Flow Speeds
Flow speed (in microchannel)
0.39 μL/min
Number of cells
55
Number of detected cells/ratio
55/100%
Flow speed (in microchannel)
0.50 μL/min
Number of cells
53
Number of detected cells/ratio
48/90.6%
Flow speed (in microchannel)
0.60 μL/min
Number of cells
46
Number of detected cells/ratio
34/74%
Flow speed (in microchannel)
0.65 μL/min
Number of cells
50
Number of detected cells/ratio
22/44%
Flow speed (in microchannel)
0.80 μL/min
Number of cells
50
Number of detected cells/ratio
0/0%
© 2009 IEEE.
calculated delay used in order to put a specific distance between each cell)
the suction mouth returns to the bottom surface and moves to the next cell,
numbered 1.
This procedure is repeated until the last cell is vacuumed from the screen
view. If the program reaches the total number of desired cells, it stops searching, moves to a safe height, and continues with the suction, even if there are
still some cells on the screen. The aspirated cells are batched in the docking area with a specific distance. The time interval between two connected
cells is given in Figure 7.9b for suction mode and supply mode. Although the
approximate absorption time for one cell to reach node A was found based
on the speed of the rotary pump and tube dimensions, the experimental
elapsed time values were different (Figure 7.11). The delay occurred due to
the drag resistance of cells as they move through the continuous fluid flow
in the Teflon tube.
7.3.2 Direction Control
Once aspiration of the desired number of cells is completed, the camera system on the cell container is toggled to the compact vision system placed on
the cross section of the “Y”-character microchannels (see Figure 7.11). The
task of the compact system is to ensure that the collected cells are gathered at
the dock and transported to the next module, by switching the valves. When
a new cell shows up in the upper right-hand corner, the program detects cells