102
5 Tailored Biointerfaces via Derivatization of Polystyrene-b-Poly …
The successful immobilization of biomolecules on PS 690 -b-PtBA 1210 films
suggests that this polymer system may be very useful in applications where tailored
biointerfaces are required. This platform may be of use to prepare robust biosensors,
to control, for example, the interaction between cells and substrates, or to facilitate
the fabrication of rapid, high-throughput genetic analysis screening biosensors, for
example DNA assays.
5.4 Cell Adhesion Studies on PS 690 -b-PtBA 1210 Films
To demonstrate that PS 690 -b-PtBA 1210 films can be used as biointerface to address
important aspects of cell membrane organization, the interaction of K562 cells [14]
with PLL and PEG 500 -NH 2 immobilized on polymer films was investigated in collaboration with Prof. M. García-Parajó (Applied Optics Group, University of Twente) by
wide-field optical microscopy. Figure 5.9a shows the surface coverage of K562 cells
on different kinds of films after applying different surface chemistry. PLL-covered
surfaces were studied first. After the film was incubated in the cell solution, cells
were imaged by wide-field optical microscopy (Fig. 5.9b). Cell surface coverages on
PLL-functionalized films are very high and cells can be found almost everywhere.
Cell–surface interaction with the neat PS 690 -b-PtBA 1210 films was also studied. For
both cases, it was found that cell morphology was not affected by the presence of
PS 690 -b-PtBA 1210 substrate.
To prevent K562 cell adhesion via non-specific interactions, hydrolyzed PS 690 -bPtBA 1210 films and PEG 500 -NH 2 functionalized PS 690 -b-PtBA 1210 films were incubated in the cell solution. For both cases, cell surface coverage has decreased largely.
Fig. 5.9 a Histogram of cell–surface coverage versus polymer surface with different compositions
is obtained from wide-field optical microscope images. b Wide-field optical micrograph of PLLcovered PS 690 -b-PtBA 1210 films (scale: 150 μm × 150 μm). c Wide-field optical micrograph of
PEG 500 -NH 2 -covered PS 690 -b-PtBA 1210 film (scale: 150 μm × 150 μm)
5 Tailored Biointerfaces via Derivatization of Polystyrene-b-Poly …
The successful immobilization of biomolecules on PS 690 -b-PtBA 1210 films
suggests that this polymer system may be very useful in applications where tailored
biointerfaces are required. This platform may be of use to prepare robust biosensors,
to control, for example, the interaction between cells and substrates, or to facilitate
the fabrication of rapid, high-throughput genetic analysis screening biosensors, for
example DNA assays.
5.4 Cell Adhesion Studies on PS 690 -b-PtBA 1210 Films
To demonstrate that PS 690 -b-PtBA 1210 films can be used as biointerface to address
important aspects of cell membrane organization, the interaction of K562 cells [14]
with PLL and PEG 500 -NH 2 immobilized on polymer films was investigated in collaboration with Prof. M. García-Parajó (Applied Optics Group, University of Twente) by
wide-field optical microscopy. Figure 5.9a shows the surface coverage of K562 cells
on different kinds of films after applying different surface chemistry. PLL-covered
surfaces were studied first. After the film was incubated in the cell solution, cells
were imaged by wide-field optical microscopy (Fig. 5.9b). Cell surface coverages on
PLL-functionalized films are very high and cells can be found almost everywhere.
Cell–surface interaction with the neat PS 690 -b-PtBA 1210 films was also studied. For
both cases, it was found that cell morphology was not affected by the presence of
PS 690 -b-PtBA 1210 substrate.
To prevent K562 cell adhesion via non-specific interactions, hydrolyzed PS 690 -bPtBA 1210 films and PEG 500 -NH 2 functionalized PS 690 -b-PtBA 1210 films were incubated in the cell solution. For both cases, cell surface coverage has decreased largely.
Fig. 5.9 a Histogram of cell–surface coverage versus polymer surface with different compositions
is obtained from wide-field optical microscope images. b Wide-field optical micrograph of PLLcovered PS 690 -b-PtBA 1210 films (scale: 150 μm × 150 μm). c Wide-field optical micrograph of
PEG 500 -NH 2 -covered PS 690 -b-PtBA 1210 film (scale: 150 μm × 150 μm)