76
3 In-Tether Chiral Center Induced Helical Peptide Modulators …
Fig. 3.12 PhR shows induction of p53 and p53 target genes in p53wt cell lines. Exponentially
growing p53wt cancer cell lines were incubated with 40 μM peptides or 1 μM nutlin-3a for 48 h,
and mRNA level of p53 and p53 targets MDM2, MDMX, and MIC or stemness-related genes
were analyzed by quantitative PCR and expressed as fold increase. Changes of gene expression in
(a) PA-1, (b) MCF-7, and (c) QSG-7701. Error bars represent SEMs for triplicates of the data, **,
P<0.01. *, P<0.05
3 In-Tether Chiral Center Induced Helical Peptide Modulators …
Fig. 3.12 PhR shows induction of p53 and p53 target genes in p53wt cell lines. Exponentially
growing p53wt cancer cell lines were incubated with 40 μM peptides or 1 μM nutlin-3a for 48 h,
and mRNA level of p53 and p53 targets MDM2, MDMX, and MIC or stemness-related genes
were analyzed by quantitative PCR and expressed as fold increase. Changes of gene expression in
(a) PA-1, (b) MCF-7, and (c) QSG-7701. Error bars represent SEMs for triplicates of the data, **,
P<0.01. *, P<0.05