34
2 Synthesis of In-Tether Chiral Center Peptides …
Fig. 2.4 Stability tests of peptide 2b. (a) Dependence on temperature (20–65°C) of full CD spectra
of 2b in PBS (PH = 7.0). (b) Mean residue ellipticity at 222 nm of 2b at different temperatures
(black line). Variation in molar ellipticity of 2b at 222 nm with increasing [guanidine HCl] at 25°C
(red line)
Table 2.2 1 H-NMR chemical shifts (δ, ppm) for peptide 1b, 2b, and 10b in H 2 O with 10% D 2 O
at 298 K
No
Residue
NH
Hα
Hβ
H (sidechain)
Peptide 1b
1
S 5 (2-Me)
8.04
4.29
–
1.62, 1.46, 1.27, 0.74
2
A
7.96
4.12
1.30
–
3
A
7.73
4.18
1.27
–
4
A
8.49
4.11
1.29
–
5
C
8.33
4.30
2.85/2.78
–
Peptide 2b
1
S 5 (2-Ph)
8.19
4.13
–
3.66, 2.89, 2.12, 1.90, 1.68, 1.29
2
A
7.99
4.13
1.47
–
3
A
7.83
4.20
1.37
–
4
A
8.48
4.15
1.35
–
5
C
8.33
4.42
3.02/2.79
–
Peptide 10b
1
S 5 (2-Me)
8.09
4.3
–
1.61, 1.41, 1.27, 1.17, 0.74
2
I
7.78
3.94
1.77
1.44, 1.11, 0.80
3
A
7.66
4.21
1.28
–
4
A
8.56
4.14
1.3
–
5
C
8.3
4.26
2.87/2.79
–