have determined that the viability of spermatozoa
decreases from 65.6 Æ 2.2% in the control group to
29.0 Æ 1.8% and 36.6 Æ 2.6% in the LN and LA groups,
respectively.
(c) An apoptosis assay can be performed by labeling with
FITC Annexin V and 7-AAD (see Fig. 4). For early apoptosis we, respectively, observed 13.8 Æ 1.5% and
14.3 Æ 1.8% for the LN and LA groups. However, an
Fig. 3 Viability of vitrified spermatozoa. (F) Fresh spermatozoa (control group),
(LN) group of spermatozoa vitrified in liquid nitrogen, and (LA) group of
spermatozoa vitrified in liquid air. A total of 200 spermatozoa were assessed.
Significant difference versus control, (∗∗∗) p < 0.001
Fig. 4 Apoptosis of vitrified spermatozoa. (F) Fresh spermatozoa (control group),
(LN) group of spermatozoa vitrified in liquid nitrogen, and (LA) group of
spermatozoa vitrified in liquid air. A total of 200 spermatozoa were assessed.
Significant difference versus control, (∗∗∗) p < 0.001
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Mengying Wang et al.