tolerance of Arabidopsis thaliana. Proc Natl
Acad Sci U S A 95:14570–14575
31. Wolkers WF, Oldenhof H, Tang F, Han J,
Bigalk J, Sieme H (2019) Factors affecting
the membrane permeability barrier function
of cells during preservation technologies.
Langmuir 35:7520–7528
32. Anchordoguy TJ, Cecchini CA, Crowe JH,
Crowe LM (1991) Insights into the cryoprotective mechanism of dimethyl sulfoxide for
phospholipid
bilayers.
Cryobiology
28:467–473
33. Prestrelski SJ, Tedeschi N, Arakawa T, Carpenter JF (1993) Dehydration-induced conformational transitions in proteins and their
inhibition by stabilizers. Biophys J
65:661–671
34. Crowe JH, Spargo BJ, Crowe LM (1987)
Preservation of dry liposomes does not
require retention of residual water. Proc Natl
Acad Sci U S A 84:1537–1540
35. Crowe JH, Oliver AE, Hoekstra FA, Crowe
LM (1997) Stabilization of dry membranes by
mixtures of hydroxyethyl starch and glucose:
the role of vitrification. Cryobiology
35:20–30
36. Leprince O, Atherton NM, Deltour R, Hendry GAF (1994) The involvement of respiration in free radical processes during loss of
desiccation tolerance in germinating Zea
mays L. An electron paramagnetic resonance
study. Plant Physiol 104:1333–1339
37. Senaratna T, McKersie BD, Borochov A
(1987) Desiccation and free radical mediated
changes in plant membranes. J Exp Bot
38:2005–2014
38. Franc ¸a MB, Panek AD, Eleutherio ECA
(2007) Oxidative stress and its effects during
dehydration. Comp Biochem Physiol A Mol
Integr Physiol 146:621–631
39. Potts M (1994) Desiccation tolerance in prokaryotes. Microbiol Rev 58:755–805
40. Murthy UMN, Sun WQ (2000) Protein modification by Amadori and Maillard reactions
during seed storage: Roles of sugar hydrolysis
and lipid peroxidation. J Exp Bot
51:1221–1228
41. Sun WQ, Leopold AC (1995) The Maillard
reaction and oxidative stress during aging of
soybean seeds. Physiol Plant 94:94–104
42. Elliott GD, Wang S, Fuller BJ (2017) Cryoprotectants: a review of the actions and applications of cryoprotective solutes that
modulate cell recovery from ultra-low temperatures. Cryobiology 76:74–91
43. Arakawa T, Timasheff SN (1985) The stabilization of proteins by osmolytes. Biophys J
47:411–414
44. Westh P (2004) Preferential interaction of
dimethyl sulfoxide and phosphatidyl choline
membranes.
Biochim
Biophys
Acta
1664:217–223
45. Oldenhof H, Friedel K, Sieme H,
Glasmacher B, Wolkers WF (2010) Freezinginduced membrane phase changes and water
transport in stallion sperm: a Fourier transform infrared spectroscopy study. Cryobiology 61:115–122
46. Akhoondi M, Oldenhof H, Stoll C, Sieme H,
Wolkers WF (2011) Membrane hydraulic permeability changes during cooling of mammalian
cells.
Biochim
Biophys
Acta
1808:642–648
47. Slade L, Levine H (1991) Beyond water activity: recent advances based on an alternative
approach to the assessment of food quality
and safety. Crit Rev Food Sci Nutr
30:115–136
48. Cordone L, Cottone G, Giuffrida S (2007)
Role of residual water hydrogen bonding in
sugar/water/biomolecule systems: a possible
explanation for trehalose peculiarity. J Physics
Condens Matter 19:205110
49. Wolkers WF, Oldenhof H, Glasmacher B
(2010) Effect of trehalose on dehydration
kinetics of phospholipid vesicles, as measured
in real time using ATR infrared spectroscopy.
Cryobiology 61:108–114
50. Wolfe J, Bryant G (1999) Freezing, drying
and/or vitrification of membrane-solutewater systems. Cryobiology 39:103–129
51. Crowe JH, Crowe LM, Wolkers WF, Oliver
AE, Ma X, Auh JH, Tang M, Zhu S, Norris J,
Tablin F (2005) Stabilization of dry mammalian cells: lessons from nature. Integr Comp
Biol 45:810–820
52. Buitink J, Leprince O (2004) Glass formation
in plant anhydrobiotes: survival in the dry
state. Cryobiology 48:215–228
53. Sydykov B, Oldenhof H, Sieme H, Wolkers
WF (2017) Hydrogen bonding interactions
and enthalpy relaxation in sugar-protein
glasses. J Pharm Sci 106:761–769
54. Oldenhof H, Zhang M, Narten K, Bigalk J,
Sydykov B, Wolkers WF, Sieme H (2017)
Freezing-induced uptake of disaccharides for
preservation of chromatin in freeze-dried
sperm during accelerated aging. Biol Reprod
97:892–890
55. Roos Y, Karel M (1991) Water and molecular
weight effects on glass transition in
22
Willem F. Wolkers and Harrie ¨ tte Oldenhof
Acad Sci U S A 95:14570–14575
31. Wolkers WF, Oldenhof H, Tang F, Han J,
Bigalk J, Sieme H (2019) Factors affecting
the membrane permeability barrier function
of cells during preservation technologies.
Langmuir 35:7520–7528
32. Anchordoguy TJ, Cecchini CA, Crowe JH,
Crowe LM (1991) Insights into the cryoprotective mechanism of dimethyl sulfoxide for
phospholipid
bilayers.
Cryobiology
28:467–473
33. Prestrelski SJ, Tedeschi N, Arakawa T, Carpenter JF (1993) Dehydration-induced conformational transitions in proteins and their
inhibition by stabilizers. Biophys J
65:661–671
34. Crowe JH, Spargo BJ, Crowe LM (1987)
Preservation of dry liposomes does not
require retention of residual water. Proc Natl
Acad Sci U S A 84:1537–1540
35. Crowe JH, Oliver AE, Hoekstra FA, Crowe
LM (1997) Stabilization of dry membranes by
mixtures of hydroxyethyl starch and glucose:
the role of vitrification. Cryobiology
35:20–30
36. Leprince O, Atherton NM, Deltour R, Hendry GAF (1994) The involvement of respiration in free radical processes during loss of
desiccation tolerance in germinating Zea
mays L. An electron paramagnetic resonance
study. Plant Physiol 104:1333–1339
37. Senaratna T, McKersie BD, Borochov A
(1987) Desiccation and free radical mediated
changes in plant membranes. J Exp Bot
38:2005–2014
38. Franc ¸a MB, Panek AD, Eleutherio ECA
(2007) Oxidative stress and its effects during
dehydration. Comp Biochem Physiol A Mol
Integr Physiol 146:621–631
39. Potts M (1994) Desiccation tolerance in prokaryotes. Microbiol Rev 58:755–805
40. Murthy UMN, Sun WQ (2000) Protein modification by Amadori and Maillard reactions
during seed storage: Roles of sugar hydrolysis
and lipid peroxidation. J Exp Bot
51:1221–1228
41. Sun WQ, Leopold AC (1995) The Maillard
reaction and oxidative stress during aging of
soybean seeds. Physiol Plant 94:94–104
42. Elliott GD, Wang S, Fuller BJ (2017) Cryoprotectants: a review of the actions and applications of cryoprotective solutes that
modulate cell recovery from ultra-low temperatures. Cryobiology 76:74–91
43. Arakawa T, Timasheff SN (1985) The stabilization of proteins by osmolytes. Biophys J
47:411–414
44. Westh P (2004) Preferential interaction of
dimethyl sulfoxide and phosphatidyl choline
membranes.
Biochim
Biophys
Acta
1664:217–223
45. Oldenhof H, Friedel K, Sieme H,
Glasmacher B, Wolkers WF (2010) Freezinginduced membrane phase changes and water
transport in stallion sperm: a Fourier transform infrared spectroscopy study. Cryobiology 61:115–122
46. Akhoondi M, Oldenhof H, Stoll C, Sieme H,
Wolkers WF (2011) Membrane hydraulic permeability changes during cooling of mammalian
cells.
Biochim
Biophys
Acta
1808:642–648
47. Slade L, Levine H (1991) Beyond water activity: recent advances based on an alternative
approach to the assessment of food quality
and safety. Crit Rev Food Sci Nutr
30:115–136
48. Cordone L, Cottone G, Giuffrida S (2007)
Role of residual water hydrogen bonding in
sugar/water/biomolecule systems: a possible
explanation for trehalose peculiarity. J Physics
Condens Matter 19:205110
49. Wolkers WF, Oldenhof H, Glasmacher B
(2010) Effect of trehalose on dehydration
kinetics of phospholipid vesicles, as measured
in real time using ATR infrared spectroscopy.
Cryobiology 61:108–114
50. Wolfe J, Bryant G (1999) Freezing, drying
and/or vitrification of membrane-solutewater systems. Cryobiology 39:103–129
51. Crowe JH, Crowe LM, Wolkers WF, Oliver
AE, Ma X, Auh JH, Tang M, Zhu S, Norris J,
Tablin F (2005) Stabilization of dry mammalian cells: lessons from nature. Integr Comp
Biol 45:810–820
52. Buitink J, Leprince O (2004) Glass formation
in plant anhydrobiotes: survival in the dry
state. Cryobiology 48:215–228
53. Sydykov B, Oldenhof H, Sieme H, Wolkers
WF (2017) Hydrogen bonding interactions
and enthalpy relaxation in sugar-protein
glasses. J Pharm Sci 106:761–769
54. Oldenhof H, Zhang M, Narten K, Bigalk J,
Sydykov B, Wolkers WF, Sieme H (2017)
Freezing-induced uptake of disaccharides for
preservation of chromatin in freeze-dried
sperm during accelerated aging. Biol Reprod
97:892–890
55. Roos Y, Karel M (1991) Water and molecular
weight effects on glass transition in
22
Willem F. Wolkers and Harrie ¨ tte Oldenhof