amorphous carbohydrates and carbohydrate
solutions. J Food Sci 56:1676–1681
56. Wolkers WF, Balasubramanian SK, Ongstad
EL, Zec H, Bischof JC (2007) Effects of
freezing on membranes and proteins in
LNCaP prostate tumor cells. Biochim Biophys Acta 1768:728–736
57. Agca Y, Mullen S, Liu J, Johnson-Ward J,
Gould K, Chan A, Critser J (2005) Osmotic
tolerance and membrane permeability characteristics of rhesus monkey (Macaca mulatta)
spermatozoa. Cryobiology 51:1–14
58. Leibo SP (1980) Water permeability and its
activation energy of fertilized and unfertilized
mouse ova. J Membr Biol 53:179–188
59. Devireddy RV, Swanlund DJ, Olin T,
Vincente W, Troedsson MHT, Bischof JC,
Roberts KP (2002) Cryopreservation of
equine sperm: optimal cooling rates in the
presence and absence of cryoprotective agents
determined using differential scanning calorimetry. Biol Reprod 66:222–231
60. Mazur P, Leibo SP, Miller RH (1974) Permeability of the bovine red cell to glycerol in
hyperosmotic solutions at various temperatures. J Membr Biol 15:107–136
61. Kleinhans FW (1998) Membrane permeability modeling: Kedem-Katchalsky vs a
two-parameter
formalism.
Cryobiology
37:271–289
62. Kedem O, Katchalsky A (1985) Thermodynamic analysis of the permeability of
biological membranes to non-electrolytes.
Biochim Biophys Acta 27:229–246
63. Benson JD (2015) Modeling and optimization of cryopreservation. In: Wolkers WF,
Oldenhof H (eds) Methods in cryopreservation and freeze-drying, methods in molecular
biology. Springer, New York, pp 83–120
64. Toupin CJ, Le Maguer M, McGann LE
(1989) Permeability of human granulocytes
to water: rectification of osmotic flow. Cryobiology 26:431–444
65. Peckys DB, Kleinhans FW, Mazur P (2011)
Rectification of the water permeability in
COS-7 cells at 22, 10 and 0
C. PLoS One 6:
e23643
66. Eroglu A, Russo MJ, Bieganski R, Fowler A,
Cheley S, Bayley H, Toner M (2000) Intracellular trehalose improves the survival of cryopreserved mammalian cells. Nat Biotechnol
18:163–167
67. Lynch AL, Chen R, Dominowski PJ, Shalaev
EY, Yancey RJ, Slater NK (2010) Biopolymer
mediated trehalose uptake for enhanced
erythrocyte
cryosurvival.
Biomaterials
31:6096–6103
68. Wei Y, Li C, Zhang L, Xu X (2014) Design of
novel cell penetrating peptides for the delivery
of trehalose into mammalian cells. Biochim
Biophys Acta 1838:1911–1920
69. Abazari A, Meimetis LG, Budin G, Bale SS,
Weissleder R, Toner M (2015) Engineered
trehalose permeable to mammalian cells.
PLoS One 10:e0130323
70. Rao W, Huang H, Wang H, Zhao S,
Dumbleton J, Zhao G, He X (2015)
Nanoparticle-mediated intracellular delivery
enables cryopreservation of human adiposederived stem cells using trehalose as the sole
cryoprotectant. ACS Appl Mater Interfaces
7:5017–5028
71. Zhang W, Rong J, Wang Q, He X (2009) The
encapsulation and intracellular delivery of trehalose using a thermally responsive nanocapsule. Nanotechnology 20:275101
72. Shirakashi R, Ko ¨stner CM, Mu ¨ller KJ,
Ku ¨rschner M, Zimmermann U, Sukhorukov
VL (2002) Intracellular delivery of trehalose
into mammalian cells by electropermeabilization. J Membr Biol 189:45–54
73. Puhlev I, Guo N, Brown DR, Levine F (2001)
Desiccation tolerance in human cells. Cryobiology 42:207–217
74. Wolkers WF, Walker NJ, Tablin F, Crowe JH
(2001) Human platelets loaded with trehalose
survive freeze-drying. Cryobiology 42:79–87
75. Beattie GM, Crowe JH, Lopez AD, Cirulli V,
Ricordi C, Hayek A (1997) Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets
after
long-term
storage.
Diabetes
46:519–523
76. Zhang M, Oldenhof H, Sieme H, Wolkers WF
(2016) Freezing-induced uptake of trehalose
into mammalian cells facilitates cryopreservation. Biochim Biophys Acta 1858:1400–1409
77. Stoll C, Holovati JL, Acker JP, Wolkers WF
(2012) Synergistic effects of liposomes, trehalose and hydroxyethyl starch for cryopreservation of human erythrocytes. Biotechnol Prog
28:364–371
78. Gl€ afke C, Akhoondi M, Oldenhof H,
Sieme H, Wolkers WF (2012) Cryopreservation of platelets using trehalose: the role of
membrane phase behavior during freezing.
Biotechnol Prog 28:1347–1354
79. Zhang M, Oldenhof H, Sydykov B, Bigalk J,
Sieme H, Wolkers WF (2017) Freeze-drying
of mammalian cells using trehalose: preservation of DNA integrity. Sci Rep 7:6198
80. Shaozhi Z, Pegg DE (2007) Analysis of the
permeation of cryoprotectants in cartilage.
Cryobiology 54:146–153
Principles of Cryopreservation and Freeze-Drying
23
solutions. J Food Sci 56:1676–1681
56. Wolkers WF, Balasubramanian SK, Ongstad
EL, Zec H, Bischof JC (2007) Effects of
freezing on membranes and proteins in
LNCaP prostate tumor cells. Biochim Biophys Acta 1768:728–736
57. Agca Y, Mullen S, Liu J, Johnson-Ward J,
Gould K, Chan A, Critser J (2005) Osmotic
tolerance and membrane permeability characteristics of rhesus monkey (Macaca mulatta)
spermatozoa. Cryobiology 51:1–14
58. Leibo SP (1980) Water permeability and its
activation energy of fertilized and unfertilized
mouse ova. J Membr Biol 53:179–188
59. Devireddy RV, Swanlund DJ, Olin T,
Vincente W, Troedsson MHT, Bischof JC,
Roberts KP (2002) Cryopreservation of
equine sperm: optimal cooling rates in the
presence and absence of cryoprotective agents
determined using differential scanning calorimetry. Biol Reprod 66:222–231
60. Mazur P, Leibo SP, Miller RH (1974) Permeability of the bovine red cell to glycerol in
hyperosmotic solutions at various temperatures. J Membr Biol 15:107–136
61. Kleinhans FW (1998) Membrane permeability modeling: Kedem-Katchalsky vs a
two-parameter
formalism.
Cryobiology
37:271–289
62. Kedem O, Katchalsky A (1985) Thermodynamic analysis of the permeability of
biological membranes to non-electrolytes.
Biochim Biophys Acta 27:229–246
63. Benson JD (2015) Modeling and optimization of cryopreservation. In: Wolkers WF,
Oldenhof H (eds) Methods in cryopreservation and freeze-drying, methods in molecular
biology. Springer, New York, pp 83–120
64. Toupin CJ, Le Maguer M, McGann LE
(1989) Permeability of human granulocytes
to water: rectification of osmotic flow. Cryobiology 26:431–444
65. Peckys DB, Kleinhans FW, Mazur P (2011)
Rectification of the water permeability in
COS-7 cells at 22, 10 and 0
C. PLoS One 6:
e23643
66. Eroglu A, Russo MJ, Bieganski R, Fowler A,
Cheley S, Bayley H, Toner M (2000) Intracellular trehalose improves the survival of cryopreserved mammalian cells. Nat Biotechnol
18:163–167
67. Lynch AL, Chen R, Dominowski PJ, Shalaev
EY, Yancey RJ, Slater NK (2010) Biopolymer
mediated trehalose uptake for enhanced
erythrocyte
cryosurvival.
Biomaterials
31:6096–6103
68. Wei Y, Li C, Zhang L, Xu X (2014) Design of
novel cell penetrating peptides for the delivery
of trehalose into mammalian cells. Biochim
Biophys Acta 1838:1911–1920
69. Abazari A, Meimetis LG, Budin G, Bale SS,
Weissleder R, Toner M (2015) Engineered
trehalose permeable to mammalian cells.
PLoS One 10:e0130323
70. Rao W, Huang H, Wang H, Zhao S,
Dumbleton J, Zhao G, He X (2015)
Nanoparticle-mediated intracellular delivery
enables cryopreservation of human adiposederived stem cells using trehalose as the sole
cryoprotectant. ACS Appl Mater Interfaces
7:5017–5028
71. Zhang W, Rong J, Wang Q, He X (2009) The
encapsulation and intracellular delivery of trehalose using a thermally responsive nanocapsule. Nanotechnology 20:275101
72. Shirakashi R, Ko ¨stner CM, Mu ¨ller KJ,
Ku ¨rschner M, Zimmermann U, Sukhorukov
VL (2002) Intracellular delivery of trehalose
into mammalian cells by electropermeabilization. J Membr Biol 189:45–54
73. Puhlev I, Guo N, Brown DR, Levine F (2001)
Desiccation tolerance in human cells. Cryobiology 42:207–217
74. Wolkers WF, Walker NJ, Tablin F, Crowe JH
(2001) Human platelets loaded with trehalose
survive freeze-drying. Cryobiology 42:79–87
75. Beattie GM, Crowe JH, Lopez AD, Cirulli V,
Ricordi C, Hayek A (1997) Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets
after
long-term
storage.
Diabetes
46:519–523
76. Zhang M, Oldenhof H, Sieme H, Wolkers WF
(2016) Freezing-induced uptake of trehalose
into mammalian cells facilitates cryopreservation. Biochim Biophys Acta 1858:1400–1409
77. Stoll C, Holovati JL, Acker JP, Wolkers WF
(2012) Synergistic effects of liposomes, trehalose and hydroxyethyl starch for cryopreservation of human erythrocytes. Biotechnol Prog
28:364–371
78. Gl€ afke C, Akhoondi M, Oldenhof H,
Sieme H, Wolkers WF (2012) Cryopreservation of platelets using trehalose: the role of
membrane phase behavior during freezing.
Biotechnol Prog 28:1347–1354
79. Zhang M, Oldenhof H, Sydykov B, Bigalk J,
Sieme H, Wolkers WF (2017) Freeze-drying
of mammalian cells using trehalose: preservation of DNA integrity. Sci Rep 7:6198
80. Shaozhi Z, Pegg DE (2007) Analysis of the
permeation of cryoprotectants in cartilage.
Cryobiology 54:146–153
Principles of Cryopreservation and Freeze-Drying
23