5. Power up the computer (see Note 32).
6. Launch the Linksys software, and select “Connect” from the
“File” menu (see Note 33). When prompted by the software to
power on the connected Linkam equipment, first push the
LNP unit’s power button and wait for its power indicator
LED to turn on, and then push the system controller’s power
button. When the power indicator LEDs of the two units are
both turned on, click “OK” at the software prompt (see Note
34).
7. Confirm that the stage temperature is displayed in the Temperature Control toolbar and that a pressure value (which should
be in the range 1–10 Pa) is displayed in the Pressure Control
toolbar; confirm that the pressure display reads “No Gauge”
when the camera trigger switch is closed.
8. Set the LNP control to manual mode, and set the flowrate to
zero (by pressing the downward arrow next to the “Lnp” box
in the Temperature Control Panel).
9. Select “Temperature Profile” from the “View” menu. Program
or open a temperature profile that specifies as its first step a
temperature value that is above room temperature (e.g., in the
range 30–40
C), with an indefinite hold (e.g., set
Time ¼ 99,999) (see Note 35). Start execution of the temperature profile by clicking the blue triangle in the Temperature
Control toolbar, and confirm that the displayed stage temperature increases to the specified value.
10. Attach the LNP tubing to the BCS196 stage, as follows. First,
attach the nitrogen withdrawal tubing (double-walled silicone
tubing) to the coolant outlet pipe on the left side of the stage
body. Next, attach the purge tubing extension (which extends
from the T-junction in the nitrogen withdrawal tubing’s
connector) to the stage chamber, by inserting the tubing’s
metal fitting into the front gas port of the BCS196 stage (see
Note 36).
11. Fill the LNP dewar with liquid nitrogen, adding the liquid a
little bit at a time by pouring from a transfer vessel (see Note
37). Fill to a level not past the beveled edge 3 cm (1.25 in.)
below the dewar rim, and wait until liquid nitrogen boiling
subsides.
12. Cap the LNP dewar using the dewar lid, by slowly immersing
the siphon tubing into the liquid nitrogen, and waiting to latch
the lid until after the liquid nitrogen boil-off has largely subsided (see Note 38).
13. Place the filled LNP dewar to the left of the microscope, and
attach the dewar’s insulated capillary tubing to the coolant
inlet pipe on the left side of the BCS196 stage body. First,
230
Jens O. M. Karlsson
6. Launch the Linksys software, and select “Connect” from the
“File” menu (see Note 33). When prompted by the software to
power on the connected Linkam equipment, first push the
LNP unit’s power button and wait for its power indicator
LED to turn on, and then push the system controller’s power
button. When the power indicator LEDs of the two units are
both turned on, click “OK” at the software prompt (see Note
34).
7. Confirm that the stage temperature is displayed in the Temperature Control toolbar and that a pressure value (which should
be in the range 1–10 Pa) is displayed in the Pressure Control
toolbar; confirm that the pressure display reads “No Gauge”
when the camera trigger switch is closed.
8. Set the LNP control to manual mode, and set the flowrate to
zero (by pressing the downward arrow next to the “Lnp” box
in the Temperature Control Panel).
9. Select “Temperature Profile” from the “View” menu. Program
or open a temperature profile that specifies as its first step a
temperature value that is above room temperature (e.g., in the
range 30–40
C), with an indefinite hold (e.g., set
Time ¼ 99,999) (see Note 35). Start execution of the temperature profile by clicking the blue triangle in the Temperature
Control toolbar, and confirm that the displayed stage temperature increases to the specified value.
10. Attach the LNP tubing to the BCS196 stage, as follows. First,
attach the nitrogen withdrawal tubing (double-walled silicone
tubing) to the coolant outlet pipe on the left side of the stage
body. Next, attach the purge tubing extension (which extends
from the T-junction in the nitrogen withdrawal tubing’s
connector) to the stage chamber, by inserting the tubing’s
metal fitting into the front gas port of the BCS196 stage (see
Note 36).
11. Fill the LNP dewar with liquid nitrogen, adding the liquid a
little bit at a time by pouring from a transfer vessel (see Note
37). Fill to a level not past the beveled edge 3 cm (1.25 in.)
below the dewar rim, and wait until liquid nitrogen boiling
subsides.
12. Cap the LNP dewar using the dewar lid, by slowly immersing
the siphon tubing into the liquid nitrogen, and waiting to latch
the lid until after the liquid nitrogen boil-off has largely subsided (see Note 38).
13. Place the filled LNP dewar to the left of the microscope, and
attach the dewar’s insulated capillary tubing to the coolant
inlet pipe on the left side of the BCS196 stage body. First,
230
Jens O. M. Karlsson