(LNP) unit, then gently turn the unit upright, and stack it on
top of the system controller unit (see Note 26).
4. Install the following cables into the designated sockets on the
back panel of the system controller unit:
(a) Connect the FDCS196 stage cable’s 25-pin connector to
the socket labeled “Stage” (see Note 27).
(b) Connect the serial communication cable to the socket
labeled “RS232.”
(c) Connect the instrument bus cable to the purple socket
labeled “Instrument Bus.”
(d) Connect the 9-pin vacuum gauge connector of the trigger
interface cable (see Subheading 3.1) to the 9-pin socket on
the vacuum controller expansion card in Slot 1.
(e) Attach the power cord to the three-prong connector.
5. Install the following cables into the designated sockets on the
back panel of the LNP unit:
(a) Connect the free end of the instrument bus cable to either
one of the two instrument bus sockets on the LNP unit.
(b) Attach the power cord to the three-prong connector.
6. Connect the free end of the serial communication cable to a
serial (COM) port on the computer, which should be powered
off before the connection is made.
7. Connect the system controller unit and the LNP unit to AC
power.
8. Insert the “G16.3” sample carrier into the cryomicroscope
stage chamber via the access door in the right side of the
stage body; close the side door and tighten the thumbscrew
to seal the chamber.
9. Attach the microscope mounting hardware (adapter plate or
clamps) to the bottom of the cryomicroscope stage.
10. Mount the cryomicroscope stage to the microscope, using the
attached mounting hardware (see Note 28).
11. Mount the high-speed video camera to the camera port on the
microscope, using an F-mount adapter (see Notes 29–31).
3.3 Experimental
Setup
1. Attach the LEMO connector (on the free end of the stage
cable) to the matching socket on the left side of the cryomicroscope stage body.
2. Attach the trigger interface cable (see Subheading 3.1) to the
camera’s trigger input connector.
3. Connect the data transfer cable (e.g., Ethernet cable) between
the camera and the computer.
4. Power up the camera by connecting its power adapter.
High-Speed Video Cryomicroscopy
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top of the system controller unit (see Note 26).
4. Install the following cables into the designated sockets on the
back panel of the system controller unit:
(a) Connect the FDCS196 stage cable’s 25-pin connector to
the socket labeled “Stage” (see Note 27).
(b) Connect the serial communication cable to the socket
labeled “RS232.”
(c) Connect the instrument bus cable to the purple socket
labeled “Instrument Bus.”
(d) Connect the 9-pin vacuum gauge connector of the trigger
interface cable (see Subheading 3.1) to the 9-pin socket on
the vacuum controller expansion card in Slot 1.
(e) Attach the power cord to the three-prong connector.
5. Install the following cables into the designated sockets on the
back panel of the LNP unit:
(a) Connect the free end of the instrument bus cable to either
one of the two instrument bus sockets on the LNP unit.
(b) Attach the power cord to the three-prong connector.
6. Connect the free end of the serial communication cable to a
serial (COM) port on the computer, which should be powered
off before the connection is made.
7. Connect the system controller unit and the LNP unit to AC
power.
8. Insert the “G16.3” sample carrier into the cryomicroscope
stage chamber via the access door in the right side of the
stage body; close the side door and tighten the thumbscrew
to seal the chamber.
9. Attach the microscope mounting hardware (adapter plate or
clamps) to the bottom of the cryomicroscope stage.
10. Mount the cryomicroscope stage to the microscope, using the
attached mounting hardware (see Note 28).
11. Mount the high-speed video camera to the camera port on the
microscope, using an F-mount adapter (see Notes 29–31).
3.3 Experimental
Setup
1. Attach the LEMO connector (on the free end of the stage
cable) to the matching socket on the left side of the cryomicroscope stage body.
2. Attach the trigger interface cable (see Subheading 3.1) to the
camera’s trigger input connector.
3. Connect the data transfer cable (e.g., Ethernet cable) between
the camera and the computer.
4. Power up the camera by connecting its power adapter.
High-Speed Video Cryomicroscopy
229
