12 Use of X-Ray Computed Tomography for Monitoring Tissue
Permeation Processes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 317
Ariadna Corral, Alberto Olmo, and Ram on Risco
13 Use of In Situ Fourier Transform Infrared Spectroscopy
in Cryobiological Research . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331
Willem F. Wolkers and Harrie ¨tte Oldenhof
14 Raman Cryomicroscopic Imaging and Sample Holder
for Spectroscopic Subzero Temperature Measurements . . . . . . . . . . . . . . . . . . . . . . 351
Guanglin Yu, Rui Li, and Allison Hubel
PART III CRYOPRESERVATION AND FREEZE-DRYING PROTOCOLS
15 Cryopreservation of Semen from Domestic Livestock: Bovine,
Equine, and Porcine Sperm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 365
Harrie ¨tte Oldenhof, Willem F. Wolkers, and Harald Sieme
16 Cryopreservation of Avian Semen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379
Henri Woelders
17 Cryopreservation of Mouse Sperm for Genome Banking . . . . . . . . . . . . . . . . . . . . 401
Yuksel Agca and Cansu Agca
18 Cryopreservation of Marine Invertebrates: From Sperm to Complex
Larval Stages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 413
Estefania Paredes, Pablo Heres, Catarina Anjos, and Elsa Cabrita
19 Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa
by Direct Dropping into a Cooling Agent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 427
Mengying Wang, Evgenia Isachenko, Gohar Rahimi,
Peter Mallmann, and Vladimir Isachenko
20 Cryopreservation of Mammalian Oocytes: Slow Cooling
and Vitrification as Successful Methods for Cryogenic Storage . . . . . . . . . . . . . . . 437
Victoria Keros and Barry J. Fuller
21 Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid
Metal Surface or Liquid Nitrogen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 455
Tamas Somfai and Kazuhiro Kikuchi
22 Cryopreservation and Transplantation of Laboratory Rodent
Ovarian Tissue for Genome Banking and Biomedical Research . . . . . . . . . . . . . . . 469
Yuksel Agca and Cansu Agca
23 Cryopreservation and Thawing of Human Ovarian Cortex Tissue
Slices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 485
Jana Liebenthron and Markus Montag
24 Vitrification: A Simple and Successful Method for Cryostorage
of Human Blastocysts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 501
Juergen Liebermann
25 Vitrification of Equine In Vivo-Derived Embryos After Blastocoel
Aspiration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 517
Carolina Herrera
26 Frozen Blood Reserves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 523
Johan W. Lagerberg
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