27 Isolation, Cryopreservation, and Characterization of iPSC-Derived
Megakaryocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 539
Denys Pogozhykh, Rainer Blasczyk, and Constanc ¸a Figueiredo
28 Chemically Defined, Clinical-Grade Cryopreservation of Human
Adipose Stem Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 555
Melany L opez and Ali Eroglu
29 Chemically Defined and Xeno-Free Cryopreservation
of Human-Induced Pluripotent Stem Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 569
Juliette Seremak and Ali Eroglu
30 Protocol for Cryopreservation of Endothelial Monolayers . . . . . . . . . . . . . . . . . . . 581
Leah A. Marquez-Curtis, Nasim Eskandari, Locksley E. McGann,
and Janet A. W. Elliott
31 Vitrification of Heart Valve Tissues. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 593
Kelvin G. M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene,
and Lia H. Campbell
32 Cryopreservation of Algae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 607
Estefania Paredes, Angela Ward, Ian Probert, Le ´na Gouhier,
and Christine N. Campbell
33 Cryopreservation of Fern Spores and Pollen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 623
Anna Nebot, Victoria J. Philpott, Anna Pajdo,
and Daniel Ballesteros
34 Cryopreservation of Plant Cell Lines Using Alginate Encapsulation . . . . . . . . . . . 639
Heinz Martin Schumacher, Martina Westphal,
and Elke Heine-Dobbernack
35 Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic,
and Shallot Using Plant Vitrification Solution 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 647
Angelika Senula and Manuela Nagel
36 Cryopreservation of Seeds and Seed Embryos in Orthodox-,
Intermediate-, and Recalcitrant-Seeded Species . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 663
Daniel Ballesteros, Natalia Fanega-Sleziak, and Rachael M. Davies
37 Freeze-Drying of Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 683
Baolin Liu and Xinli Zhou
38 Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach
for Developing a Freeze-Drying Protocol Based on Physical Properties. . . . . . . . 703
Fernanda Fonseca, Ame ´lie Girardeau, and Ste ´phanie Passot
39 Preservation of Mammalian Sperm by Freeze-Drying . . . . . . . . . . . . . . . . . . . . . . . 721
Levent Keskintepe and Ali Eroglu
40 Freeze-Drying of Decellularized Heart Valves
for Off-the-Shelf Availability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 731
Willem F. Wolkers and Andres Hilfiker
Correction to: Freezing Technology: Control of Freezing, Thawing,
and Ice Nucleation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C1
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 741
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