Contents
Dedication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
v
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
vii
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
PART I FUNDAMENTAL ASPECTS OF CRYOPRESERVATION AND FREEZE-DRYING
1 Principles Underlying Cryopreservation and Freeze-Drying
of Cells and Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3
Willem F. Wolkers and Harrie ¨tte Oldenhof
2 Principles of Ice-Free Cryopreservation by Vitrification . . . . . . . . . . . . . . . . . . . . . . 27
Gregory M. Fahy and Brian Wowk
3 The Principles of Freeze-Drying and Application of Analytical
Technologies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Kevin R. Ward and Paul Matejtschuk
4 Mathematical Modeling and Optimization of Cryopreservation
in Single Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
James D. Benson
5 Mathematical Modeling of Protectant Transport in Tissues . . . . . . . . . . . . . . . . . . 173
Ross M. Warner and Adam Z. Higgins
PART II TECHNOLOGIES AND METHODS TO STUDY FREEZING AND DRYING
6 Freezing Technology: Control of Freezing, Thawing,
and Ice Nucleation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 191
Peter Kilbride and Julie Meneghel
7 Microwave- and Laser-Assisted Drying for the Anhydrous
Preservation of Biologics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203
Shangping Wang, Susan Trammell, and Gloria D. Elliott
8 High-Speed Video Cryomicroscopy for Measurement
of Intracellular Ice Formation Kinetics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 221
Jens O. M. Karlsson
9 Use of Ice Recrystallization Inhibition Assays to Screen
for Compounds That Inhibit Ice Recrystallization . . . . . . . . . . . . . . . . . . . . . . . . . . 271
Anna A. Ampaw, August Sibthorpe, and Robert N. Ben
10 DSC Analysis of Thermophysical Properties for Biomaterials
and Formulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 285
Wendell Q. Sun
11 Osmometric Measurements of Cryoprotective Agent
Permeation into Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 303
Kezhou Wu, Leila Laouar, Nadia Shardt, Janet A. W. Elliott,
and Nadr M. Jomha
ix
Dedication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
v
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
vii
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
PART I FUNDAMENTAL ASPECTS OF CRYOPRESERVATION AND FREEZE-DRYING
1 Principles Underlying Cryopreservation and Freeze-Drying
of Cells and Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3
Willem F. Wolkers and Harrie ¨tte Oldenhof
2 Principles of Ice-Free Cryopreservation by Vitrification . . . . . . . . . . . . . . . . . . . . . . 27
Gregory M. Fahy and Brian Wowk
3 The Principles of Freeze-Drying and Application of Analytical
Technologies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Kevin R. Ward and Paul Matejtschuk
4 Mathematical Modeling and Optimization of Cryopreservation
in Single Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
James D. Benson
5 Mathematical Modeling of Protectant Transport in Tissues . . . . . . . . . . . . . . . . . . 173
Ross M. Warner and Adam Z. Higgins
PART II TECHNOLOGIES AND METHODS TO STUDY FREEZING AND DRYING
6 Freezing Technology: Control of Freezing, Thawing,
and Ice Nucleation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 191
Peter Kilbride and Julie Meneghel
7 Microwave- and Laser-Assisted Drying for the Anhydrous
Preservation of Biologics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203
Shangping Wang, Susan Trammell, and Gloria D. Elliott
8 High-Speed Video Cryomicroscopy for Measurement
of Intracellular Ice Formation Kinetics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 221
Jens O. M. Karlsson
9 Use of Ice Recrystallization Inhibition Assays to Screen
for Compounds That Inhibit Ice Recrystallization . . . . . . . . . . . . . . . . . . . . . . . . . . 271
Anna A. Ampaw, August Sibthorpe, and Robert N. Ben
10 DSC Analysis of Thermophysical Properties for Biomaterials
and Formulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 285
Wendell Q. Sun
11 Osmometric Measurements of Cryoprotective Agent
Permeation into Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 303
Kezhou Wu, Leila Laouar, Nadia Shardt, Janet A. W. Elliott,
and Nadr M. Jomha
ix