5. Capture videos of the particle motion, usually at 60 s durations
and at a frame rate of 30 frames/s within the chamber upon the
application of 10 V, under both positive and negative polarity.
Maintain temperature at 25
C during measurements, with an
assumed sample viscosity of 0.9 cP and dielectric constant of
80.0. A typical result obtained from NTA can be seen in Figs. 4
and 5. See Note 25 for discussion about differences that may
occur with respect to nanocomplex sizes using TEM and NTA.
2.5
1.5
0.5
8.0
7.0
6.0
5.0
4.0
3.0
2.0
1.0
0
0
2.0
1.0
0
500 1000
2000
Size (nm)
3000
4000
1500
2500
3500
–200 –150
–100
–50
50
100
200
150
0
Zeta Potential (mV)
Concentration (E6 particles/mL)
Concentration (E6 particles/mL)
Fig. 4 NTA profile showing the size distribution and zeta potential of a siRNA:
functionalized gold nanocomplex
50
Moganavelli Singh
and at a frame rate of 30 frames/s within the chamber upon the
application of 10 V, under both positive and negative polarity.
Maintain temperature at 25
C during measurements, with an
assumed sample viscosity of 0.9 cP and dielectric constant of
80.0. A typical result obtained from NTA can be seen in Figs. 4
and 5. See Note 25 for discussion about differences that may
occur with respect to nanocomplex sizes using TEM and NTA.
2.5
1.5
0.5
8.0
7.0
6.0
5.0
4.0
3.0
2.0
1.0
0
0
2.0
1.0
0
500 1000
2000
Size (nm)
3000
4000
1500
2500
3500
–200 –150
–100
–50
50
100
200
150
0
Zeta Potential (mV)
Concentration (E6 particles/mL)
Concentration (E6 particles/mL)
Fig. 4 NTA profile showing the size distribution and zeta potential of a siRNA:
functionalized gold nanocomplex
50
Moganavelli Singh