4 Notes
1. Ethidium bromide in solution can be purchased commercially.
2. To facilitate better solubility, adjust pH to 8.0 with NaOH.
3. The gel loading buffer can also be prepared by adding 25 mg
bromophenol blue and 4 g sucrose; or 25 mg bromophenol
blue, 25 mg xylene cyanol, and 3 mL glycerol in 10 mL H 2 O.
4. Other buffers such a Tris-borate-EDTA (TBE) can also be used
as effectively if available in your laboratory.
0
100 200
300 400 500
Size (nm)
600
700 800 900 1000
55.29
8.53
–200
–150
–100
–50
0
Zeta Potential (mV)
50
100
200
150
Concentration (E6 particles/mL)
Concentration (E6 particles/mL)
Fig. 5 NTA profile showing the size distribution and zeta potential of a DNA:
liposome nanocomplex
Nucleic Acid:Nanoparticle Interactions
51
1. Ethidium bromide in solution can be purchased commercially.
2. To facilitate better solubility, adjust pH to 8.0 with NaOH.
3. The gel loading buffer can also be prepared by adding 25 mg
bromophenol blue and 4 g sucrose; or 25 mg bromophenol
blue, 25 mg xylene cyanol, and 3 mL glycerol in 10 mL H 2 O.
4. Other buffers such a Tris-borate-EDTA (TBE) can also be used
as effectively if available in your laboratory.
0
100 200
300 400 500
Size (nm)
600
700 800 900 1000
55.29
8.53
–200
–150
–100
–50
0
Zeta Potential (mV)
50
100
200
150
Concentration (E6 particles/mL)
Concentration (E6 particles/mL)
Fig. 5 NTA profile showing the size distribution and zeta potential of a DNA:
liposome nanocomplex
Nucleic Acid:Nanoparticle Interactions
51