including aluminum chloride and calcium chloride, complex coacervation, and irradiation cross-linking or combinations of abovementioned approaches.
In this chapter, the fabrication aspects of polysaccharide-based
hydrogel beads as potential carriers for colon-targeted drug delivery will be discussed.
2 Materials
2.1 General
Chemicals
and Reagents
All reagents used should be of analytical grade.
1. Methanol.
2. Isopropanol (99.9%).
3. Ethanol (denatured 95%).
4. Sodium chlorite (80%).
5. Sodium monochloroacetate (98%).
6. Sodium hydroxide pellets (97%).
7. Glacial acetic acid (99.9%).
8. Acetone (99.6%).
9. Chloroform.
10. Formic acid.
11. Hydrogen peroxide (30%).
12. Chloroform.
13. Sulfuric acid (98.7%).
14. Formic acid.
15. Phenolphthalein.
16. 0.9% Saline: Dissolve 0.9 g NaCl in 70 mL deionized water,
and bring up to 100 mL.
17. Hielscher UIP500hd ultrasonic homogenizer.
2.2 Materials
and Equipment
1. Automated 3 Â 1 systematic multi hot plate stirrer (e.g.,
WiseStir, model SMHS-3, Korea).
2. Buchi encapsulator that meets the following technical specifications: (2 mm nozzle, 10 cm distance between nozzle and
solution, flow rate of 0.8 mL/min, electrode voltage of
900 mV, vibration frequency of 1700 Hz, temperature: 35
C
[8]; 750 μm nozzle, air pressure of 500–700 mbar, electrode
voltage of 1000 mV, vibration frequency of 600 Hz [9].
3. 3 mL (diameter 8.95 mm) and 5 mL (diameter 13 mm) plastic
syringes.
4. 18- and 21-gauge needle tips.
174
Janarthanan Pushpamalar et al.
In this chapter, the fabrication aspects of polysaccharide-based
hydrogel beads as potential carriers for colon-targeted drug delivery will be discussed.
2 Materials
2.1 General
Chemicals
and Reagents
All reagents used should be of analytical grade.
1. Methanol.
2. Isopropanol (99.9%).
3. Ethanol (denatured 95%).
4. Sodium chlorite (80%).
5. Sodium monochloroacetate (98%).
6. Sodium hydroxide pellets (97%).
7. Glacial acetic acid (99.9%).
8. Acetone (99.6%).
9. Chloroform.
10. Formic acid.
11. Hydrogen peroxide (30%).
12. Chloroform.
13. Sulfuric acid (98.7%).
14. Formic acid.
15. Phenolphthalein.
16. 0.9% Saline: Dissolve 0.9 g NaCl in 70 mL deionized water,
and bring up to 100 mL.
17. Hielscher UIP500hd ultrasonic homogenizer.
2.2 Materials
and Equipment
1. Automated 3 Â 1 systematic multi hot plate stirrer (e.g.,
WiseStir, model SMHS-3, Korea).
2. Buchi encapsulator that meets the following technical specifications: (2 mm nozzle, 10 cm distance between nozzle and
solution, flow rate of 0.8 mL/min, electrode voltage of
900 mV, vibration frequency of 1700 Hz, temperature: 35
C
[8]; 750 μm nozzle, air pressure of 500–700 mbar, electrode
voltage of 1000 mV, vibration frequency of 600 Hz [9].
3. 3 mL (diameter 8.95 mm) and 5 mL (diameter 13 mm) plastic
syringes.
4. 18- and 21-gauge needle tips.
174
Janarthanan Pushpamalar et al.