14
J. Chen et al.
Moisturizing is usually done by placing moist filter paper under identified plant tissues and organs, or by artificially controlling humidity in the air by receiving insects
in an insect culture room. In addition, in the process of receiving insects, we should
try our best to avoid the damage to the insect body, otherwise it will have a great
impact on the results of the experiment.
Identification of outdoor cage insects: the identification of outdoor cage insects is
to artificially cover the yarn net for the whole plant or a part of the plant that grows in
potted plants or in the field. In order to isolate the natural enemies of identified pests
and other pests, and to create an environment for the feeding of identified pests. You
can also use a fixed network room or in the greenhouse for identification, can achieve
the same effect. The number and mode of receiving insects should be determined
according to different crops, different pests and different experimental purposes,
and there is no fixed model. In the process of identification, it is also necessary to
investigate the victimization of plants, the death and development of insects, and so
on. At the same time, there should be controls that can be combined in a single way
or in two ways. For a single way, indoor biological identification is generally used.
Identification of naturally susceptible insects in the field: plants must grow under
natural conditions and identify insect resistance under natural conditions in order to
be meaningful. The identification of naturally susceptible insects in the field, also
known as the field control investigation method, is the identification method that can
achieve this goal, but this identification method is restricted by the population size of
transgenic plants, and the small population cannot be identified in the field. Only if
the population is large enough to identify naturally susceptible insects in the field can
it be persuasive. It should be noted that because the field is an open environment, it
is vulnerable to natural factors such as weather conditions, insect population, natural
enemies and so on. This requires us to do field insect identification in a variety of
areas, long time identification, to spend a lot of manpower, material and financial
resources. Therefore, field identification requires not only enough population size,
but also enough strength to ensure.
Insect resistance classification: according to the harm characteristics of pests,
determine the location of the investigation, such as the number of leaves, stems, fruit
or pest caused by the fruit drop rate. According to the characteristics of different
industrial oil plant pests, the damage degree was divided into several grades, usually
divided into 0 ≤ 9 grades, which were resistant to (mr), and (hs) in (r), with high
resistance to (hr), and so on. The number of plants at all levels was counted, and the
pest index was calculated according to the following formula, and then the insect
resistance of germline materials was judged according to the pest index. Pest index
= 100% ×
(representative grade value × number of plants or leaves)/(number of
highest values × number of total plants or leaves).
Cold resistance evaluation: temperature is the dominant factor affecting the geographical distribution of industrial oil plants in climatic factors. Under the premise
of the limitation of genetic background of industrial oil plants, it plays a decisive
role in some growth and development processes of industrial oil plants. Low temperature stress is a common disaster in the cultivation of industrial oil plants. The cold
resistance of industrial oil plants determines its growing season and planting range.
J. Chen et al.
Moisturizing is usually done by placing moist filter paper under identified plant tissues and organs, or by artificially controlling humidity in the air by receiving insects
in an insect culture room. In addition, in the process of receiving insects, we should
try our best to avoid the damage to the insect body, otherwise it will have a great
impact on the results of the experiment.
Identification of outdoor cage insects: the identification of outdoor cage insects is
to artificially cover the yarn net for the whole plant or a part of the plant that grows in
potted plants or in the field. In order to isolate the natural enemies of identified pests
and other pests, and to create an environment for the feeding of identified pests. You
can also use a fixed network room or in the greenhouse for identification, can achieve
the same effect. The number and mode of receiving insects should be determined
according to different crops, different pests and different experimental purposes,
and there is no fixed model. In the process of identification, it is also necessary to
investigate the victimization of plants, the death and development of insects, and so
on. At the same time, there should be controls that can be combined in a single way
or in two ways. For a single way, indoor biological identification is generally used.
Identification of naturally susceptible insects in the field: plants must grow under
natural conditions and identify insect resistance under natural conditions in order to
be meaningful. The identification of naturally susceptible insects in the field, also
known as the field control investigation method, is the identification method that can
achieve this goal, but this identification method is restricted by the population size of
transgenic plants, and the small population cannot be identified in the field. Only if
the population is large enough to identify naturally susceptible insects in the field can
it be persuasive. It should be noted that because the field is an open environment, it
is vulnerable to natural factors such as weather conditions, insect population, natural
enemies and so on. This requires us to do field insect identification in a variety of
areas, long time identification, to spend a lot of manpower, material and financial
resources. Therefore, field identification requires not only enough population size,
but also enough strength to ensure.
Insect resistance classification: according to the harm characteristics of pests,
determine the location of the investigation, such as the number of leaves, stems, fruit
or pest caused by the fruit drop rate. According to the characteristics of different
industrial oil plant pests, the damage degree was divided into several grades, usually
divided into 0 ≤ 9 grades, which were resistant to (mr), and (hs) in (r), with high
resistance to (hr), and so on. The number of plants at all levels was counted, and the
pest index was calculated according to the following formula, and then the insect
resistance of germline materials was judged according to the pest index. Pest index
= 100% ×
(representative grade value × number of plants or leaves)/(number of
highest values × number of total plants or leaves).
Cold resistance evaluation: temperature is the dominant factor affecting the geographical distribution of industrial oil plants in climatic factors. Under the premise
of the limitation of genetic background of industrial oil plants, it plays a decisive
role in some growth and development processes of industrial oil plants. Low temperature stress is a common disaster in the cultivation of industrial oil plants. The cold
resistance of industrial oil plants determines its growing season and planting range.