1 Introduction
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Cold resistance of industrial oil plants refers to the ability of industrial oil energy
plants to endure and resist low temperature, and the harm of low temperature to
industrial oil plants, according to the degree of low temperature and damage. It can
be divided into cold injury (zero upper and lower temperature) and freezing injury
(subzero low temperature). Although there is no freezing phenomenon above zero
temperature, it can cause physiological obstacles of warm industrial oil plants and
cause them to be injured or even killed. The adaptability of industrial oil plants to
low temperatures above zero is called cold resistance. When the temperature drops
below zero, the industrial oil plant freezes, so it is injured or even death occurs. Its
adaptability to low temperature below zero is called freezing resistance.
Field identification: the plant species exposed to natural conditions are used as
the research object, and after the low temperature test in winter, the plant species can
be observed from the external character, or some index of the plant can be determined
by means of the instrument, so as to investigate the frost damage and the survival
condition of the plant, thereby identifying the cold resistance of the plant.
Indoor identification method: by means of artificial refrigeration equipment, the
identified plant material is subjected to freezing treatment under a certain freezing
condition, and the survival and freezing conditions of the plant material are observed
and detected by a certain method. According to its detection principle, it can be
divided into physiological and biochemical research, molecular biology research and
physical research. The physiological and biochemical changes, such as the change
of photosynthesis and respiration, soluble sugar, soluble protein, free amino acid and
other organic acid levels and the change of the hormone level. The cold resistance
of different plants can be compared by the determination of these indexes, which
mainly include gas chromatography, spectrophotometry and isoenzyme method. The
physical method mainly includes the recovery of the growth method, the electric conductivity method and the microscopic examination method. The biological research
mainly includes the dna molecular marker and the cold-resistant gene clone.
Cold resistance evaluation index and classification: according to the criteria of
the internal column standard, the cold resistance force of the industrial oilseed plants
with different species and varieties is divided into five grades, and the cold resistance
force of the plant is the strongest. The whole plant has no freezing damage, the victim
rate of the bud is below 101%, or the length of the branch of the branch is less than
10%, and the cold resistance of the second grade is strong. The tree trunk has no
freezing damage, the victim rate of the bud is about 30%, or the length of the branch
is less than 50%, and the cold resistance of the third grade is moderate. The frost
damage of the trunk is less than 50%, the injury rate of the bud is less than 70% and
the frostbite length of the branch is below 50%. The cold resistance of the 4-stage
cold resistance is weak. The frost damage of the trunk is more than 50%, the rate of
the bud is 70% or the branch is all frostbite, but the next year can still germinate. All
or a large part of the above-ground part of the ground is frozen, the victim rate of the
bud is above 90%, or the root can still germinate in the next year.
Drought resistance evaluation: plant drought resistance refers to the adaptability
of plants to drought, that is to say, the ability of plants to grow, survive and reproduce in arid environment, as well as the ability of plant yield recovery after drought
15
Cold resistance of industrial oil plants refers to the ability of industrial oil energy
plants to endure and resist low temperature, and the harm of low temperature to
industrial oil plants, according to the degree of low temperature and damage. It can
be divided into cold injury (zero upper and lower temperature) and freezing injury
(subzero low temperature). Although there is no freezing phenomenon above zero
temperature, it can cause physiological obstacles of warm industrial oil plants and
cause them to be injured or even killed. The adaptability of industrial oil plants to
low temperatures above zero is called cold resistance. When the temperature drops
below zero, the industrial oil plant freezes, so it is injured or even death occurs. Its
adaptability to low temperature below zero is called freezing resistance.
Field identification: the plant species exposed to natural conditions are used as
the research object, and after the low temperature test in winter, the plant species can
be observed from the external character, or some index of the plant can be determined
by means of the instrument, so as to investigate the frost damage and the survival
condition of the plant, thereby identifying the cold resistance of the plant.
Indoor identification method: by means of artificial refrigeration equipment, the
identified plant material is subjected to freezing treatment under a certain freezing
condition, and the survival and freezing conditions of the plant material are observed
and detected by a certain method. According to its detection principle, it can be
divided into physiological and biochemical research, molecular biology research and
physical research. The physiological and biochemical changes, such as the change
of photosynthesis and respiration, soluble sugar, soluble protein, free amino acid and
other organic acid levels and the change of the hormone level. The cold resistance
of different plants can be compared by the determination of these indexes, which
mainly include gas chromatography, spectrophotometry and isoenzyme method. The
physical method mainly includes the recovery of the growth method, the electric conductivity method and the microscopic examination method. The biological research
mainly includes the dna molecular marker and the cold-resistant gene clone.
Cold resistance evaluation index and classification: according to the criteria of
the internal column standard, the cold resistance force of the industrial oilseed plants
with different species and varieties is divided into five grades, and the cold resistance
force of the plant is the strongest. The whole plant has no freezing damage, the victim
rate of the bud is below 101%, or the length of the branch of the branch is less than
10%, and the cold resistance of the second grade is strong. The tree trunk has no
freezing damage, the victim rate of the bud is about 30%, or the length of the branch
is less than 50%, and the cold resistance of the third grade is moderate. The frost
damage of the trunk is less than 50%, the injury rate of the bud is less than 70% and
the frostbite length of the branch is below 50%. The cold resistance of the 4-stage
cold resistance is weak. The frost damage of the trunk is more than 50%, the rate of
the bud is 70% or the branch is all frostbite, but the next year can still germinate. All
or a large part of the above-ground part of the ground is frozen, the victim rate of the
bud is above 90%, or the root can still germinate in the next year.
Drought resistance evaluation: plant drought resistance refers to the adaptability
of plants to drought, that is to say, the ability of plants to grow, survive and reproduce in arid environment, as well as the ability of plant yield recovery after drought