88
K. Igarashi and H. Ooshima
Fig. 5.4 Schematic diagrams of the mL-scale crystallizer. 1. Mixing vessel, 2. high speed agitator,
3. dispersing element, 4. plastic syringes, 5. syringe pump, 6. inlet tubes, 7. outlet tube, 8. glass
vessel with an water jacket, and 9. enlarged illustration of the mixing vessel
5.3.2 Apparatus
Figure 5.4 shows a schematic diagram of the mL-scale crystallizer. The crystallizer is
composed of a stainless-steel vessel, a high-speed agitator (Ultra-turrax T25 homogenizer, IKA), and a syringe pump (IC3210, KD Scientific). The maximum rotation
speed of homogenizer is 24,000 rpm. An agitation shaft is inserted from the top of
the vessel and fixed by a screw lid and O-ring. The solute solution and the antisolvent
are supplied from two inlet tubes by the syringe pump and intensely mixed with the
agitator. The void volume of the crystallizer is 0.9 mL, respectively. The slurry is
recovered from an outlet tube at the side of the vessel.
5.3.3 Crystallization of Glycine and Alanine Crystals
Amino acids, glycine, and alanine, were crystallized using the mL-crystallizer. The
initial concentration of l-alanine and glycine were 166 mg/mL and 250 mg/mL,
K. Igarashi and H. Ooshima
Fig. 5.4 Schematic diagrams of the mL-scale crystallizer. 1. Mixing vessel, 2. high speed agitator,
3. dispersing element, 4. plastic syringes, 5. syringe pump, 6. inlet tubes, 7. outlet tube, 8. glass
vessel with an water jacket, and 9. enlarged illustration of the mixing vessel
5.3.2 Apparatus
Figure 5.4 shows a schematic diagram of the mL-scale crystallizer. The crystallizer is
composed of a stainless-steel vessel, a high-speed agitator (Ultra-turrax T25 homogenizer, IKA), and a syringe pump (IC3210, KD Scientific). The maximum rotation
speed of homogenizer is 24,000 rpm. An agitation shaft is inserted from the top of
the vessel and fixed by a screw lid and O-ring. The solute solution and the antisolvent
are supplied from two inlet tubes by the syringe pump and intensely mixed with the
agitator. The void volume of the crystallizer is 0.9 mL, respectively. The slurry is
recovered from an outlet tube at the side of the vessel.
5.3.3 Crystallization of Glycine and Alanine Crystals
Amino acids, glycine, and alanine, were crystallized using the mL-crystallizer. The
initial concentration of l-alanine and glycine were 166 mg/mL and 250 mg/mL,