46. A t-test or one-way ANOVA test should be used to compare
the different locations to each other. For two data sets the type
of t-test depends on the results of the variance test. For equal
variances, use a two-sample t-test. If the variances are not
equal, perform a paired t-test. For more than two data sets
the type of test depends on the results of the variance test. For
equal variances, use a one-way ANOVA test. If variances are not
equal, evaluate the data sets two at a time using a two-sample ttest.
47. Environmental conditions and light requirements for seed germination tests are the same as for the growth of plants.
References
1. Knee E, Rivero L, Crist D, Grotewold E, Scholl
R (2011) Germplasm and molecular resources.
In: Schmidt R, Bancroft I (eds) Plant genetics
and genomics: crops and models, vol
9. Springer, New York, pp 437–467
2. Axelos M, Curie C, Mazzolini L, Bardet C,
Lescure B (1992) A protocol for transient
gene-expression in Arabidopsis thaliana protoplasts isolated from cell-suspension cultures.
Plant Physiol Biochem 30:123–128
3. Yamada H, Koizumi N, Nakamichi N, Kiba T,
Yamashino T, Mizuno T (2004) Rapid
response of Arabidopsis T87 cultured cells to
cytokinin through His-to-Asp phosphorelay
signal transduction. Biosci Biotechnol Biochem 68:1966–1976
4. Nakamichi N, Matsushika A, Yamashino T,
Mizuno T (2003) Cell autonomous circadian
waves of the APRR1/TOC1 quintet in an
established cell line of Arabidopsis thaliana.
Plant Cell Physiol 44:360–365
5. Alonso AP, Piasecki RJ, Wang Y, LaClair RW,
Shachar-Hill Y (2010) Quantifying the labeling
and the levels of plant cell wall precursors using
ion chromatography tandem mass spectrometry. Plant Physiol 153:915–924
6. Ogawa Y, Dansako T, Yano K, Sakurai N,
Suzuki H, Aoki K, Noji M, Saito K, Shibata D
(2008) Efficient and high-throughput vector
construction and Agrobacterium-mediated
transformation of Arabidopsis thaliana
suspension-cultured cells for functional genomics. Plant Cell Physiol 49:242–250
7. Rivero L, Scholl R, Holomuzki N, Crist D,
Grotewold E, Brkljacic J (2014) Handling Arabidopsis plants: growth, preservation of seeds,
transformation, and genetic crosses. In:
Sanchez-Serrano J, Salinas J (eds) Arabidopsis
protocols, Methods in molecular biology, vol
1062. Humana Press, Totowa, pp 3–25
8. McGinn M, Phippen WB, Chopra R, Bansal S,
Jarvis BA, Phippen ME, Dorn KM,
Esfahanian M, Nazarenus TJ, Cahoon EB,
Durrett TP, Marks MD, Sedbrook JC (2018)
Molecular tools enabling pennycress (Thlaspi
arvense) as a model plant and oilseed cash
cover crop. Plant Biotechnol J 17:776–788
9. An M, Zeng L, Zhang T, Zhong Y (2015)
Phylogeography of Thlaspi arvense (Brassicaceae) in China inferred from chloroplast and
nuclear DNA sequences and ecological niche
modeling. Int J Mol Sci 16:13339–13355
10. Walters C (1998) Understanding the mechanisms and kinetics of seed aging. Seed Sci Res
8:223–244
11. Walters C (1998) Ultra-dry seed storage. Seed
Sci Res 8:1–73
12. Rivero-Lepinckas L, Crist D, Scholl R (2006)
Growth of plants and preservation of seeds. In:
Salinas J, Sanchez-Serrano JJ (eds) Methods in
molecular biology, Arabidopsis protocols, vol
323. Humana Press, Totowa, pp 3–12
13. Rao NK, Hanson J, Dulloo ME, Ghosh K,
Nowell D, Larinde M (2006) Manual of seed
handling in genebanks. Bioversity International, Rome
14. Hong TD, Ellis RH (1996) A protocol to
determine seed storage behavior. IPGRI,
Rome
15. FAO and IPGRI (1994) Genebank standards.
FAO, IPGRI, Rome, pp 1–8
16. Ooms J, Leon-Kloosterziel KM, Bartels D,
Koornneef M, Karssen CM (1993) Acquisition
of desiccation tolerance and longevity in seeds
of Arabidopsis thaliana (a comparative study
using Abscisic acid-insensitive abi3 mutants).
Plant Physiol 102:1185–1191
17. Hay FR, Mead A, Manger K, Wilson FJ (2003)
One-step analysis of seed storage data and the
22
Christopher Calhoun et al.
the different locations to each other. For two data sets the type
of t-test depends on the results of the variance test. For equal
variances, use a two-sample t-test. If the variances are not
equal, perform a paired t-test. For more than two data sets
the type of test depends on the results of the variance test. For
equal variances, use a one-way ANOVA test. If variances are not
equal, evaluate the data sets two at a time using a two-sample ttest.
47. Environmental conditions and light requirements for seed germination tests are the same as for the growth of plants.
References
1. Knee E, Rivero L, Crist D, Grotewold E, Scholl
R (2011) Germplasm and molecular resources.
In: Schmidt R, Bancroft I (eds) Plant genetics
and genomics: crops and models, vol
9. Springer, New York, pp 437–467
2. Axelos M, Curie C, Mazzolini L, Bardet C,
Lescure B (1992) A protocol for transient
gene-expression in Arabidopsis thaliana protoplasts isolated from cell-suspension cultures.
Plant Physiol Biochem 30:123–128
3. Yamada H, Koizumi N, Nakamichi N, Kiba T,
Yamashino T, Mizuno T (2004) Rapid
response of Arabidopsis T87 cultured cells to
cytokinin through His-to-Asp phosphorelay
signal transduction. Biosci Biotechnol Biochem 68:1966–1976
4. Nakamichi N, Matsushika A, Yamashino T,
Mizuno T (2003) Cell autonomous circadian
waves of the APRR1/TOC1 quintet in an
established cell line of Arabidopsis thaliana.
Plant Cell Physiol 44:360–365
5. Alonso AP, Piasecki RJ, Wang Y, LaClair RW,
Shachar-Hill Y (2010) Quantifying the labeling
and the levels of plant cell wall precursors using
ion chromatography tandem mass spectrometry. Plant Physiol 153:915–924
6. Ogawa Y, Dansako T, Yano K, Sakurai N,
Suzuki H, Aoki K, Noji M, Saito K, Shibata D
(2008) Efficient and high-throughput vector
construction and Agrobacterium-mediated
transformation of Arabidopsis thaliana
suspension-cultured cells for functional genomics. Plant Cell Physiol 49:242–250
7. Rivero L, Scholl R, Holomuzki N, Crist D,
Grotewold E, Brkljacic J (2014) Handling Arabidopsis plants: growth, preservation of seeds,
transformation, and genetic crosses. In:
Sanchez-Serrano J, Salinas J (eds) Arabidopsis
protocols, Methods in molecular biology, vol
1062. Humana Press, Totowa, pp 3–25
8. McGinn M, Phippen WB, Chopra R, Bansal S,
Jarvis BA, Phippen ME, Dorn KM,
Esfahanian M, Nazarenus TJ, Cahoon EB,
Durrett TP, Marks MD, Sedbrook JC (2018)
Molecular tools enabling pennycress (Thlaspi
arvense) as a model plant and oilseed cash
cover crop. Plant Biotechnol J 17:776–788
9. An M, Zeng L, Zhang T, Zhong Y (2015)
Phylogeography of Thlaspi arvense (Brassicaceae) in China inferred from chloroplast and
nuclear DNA sequences and ecological niche
modeling. Int J Mol Sci 16:13339–13355
10. Walters C (1998) Understanding the mechanisms and kinetics of seed aging. Seed Sci Res
8:223–244
11. Walters C (1998) Ultra-dry seed storage. Seed
Sci Res 8:1–73
12. Rivero-Lepinckas L, Crist D, Scholl R (2006)
Growth of plants and preservation of seeds. In:
Salinas J, Sanchez-Serrano JJ (eds) Methods in
molecular biology, Arabidopsis protocols, vol
323. Humana Press, Totowa, pp 3–12
13. Rao NK, Hanson J, Dulloo ME, Ghosh K,
Nowell D, Larinde M (2006) Manual of seed
handling in genebanks. Bioversity International, Rome
14. Hong TD, Ellis RH (1996) A protocol to
determine seed storage behavior. IPGRI,
Rome
15. FAO and IPGRI (1994) Genebank standards.
FAO, IPGRI, Rome, pp 1–8
16. Ooms J, Leon-Kloosterziel KM, Bartels D,
Koornneef M, Karssen CM (1993) Acquisition
of desiccation tolerance and longevity in seeds
of Arabidopsis thaliana (a comparative study
using Abscisic acid-insensitive abi3 mutants).
Plant Physiol 102:1185–1191
17. Hay FR, Mead A, Manger K, Wilson FJ (2003)
One-step analysis of seed storage data and the
22
Christopher Calhoun et al.