longevity of Arabidopsis thaliana seeds. J Exp
Bot 54:993–1011
18. Koornneef M (1994) Arabidopsis genetics. In:
Meyerowitz E, Somerville C (eds) Arabidopsis.
Cold Spring Harbor Laboratory Press, Cold
Spring Harbor, pp 89–120
19. Nasrallah J (2011) Self-incompatibility in the
brassicaceae. In: Schmidt R, Bancroft I (eds)
Plant genetics and genomics: crops and models, genetics and genomics of the Brassicaceae,
vol 9. Springer, New York, pp 389–411
20. Clough SJ, Bent AF (1998) Floral dip: a simplified method for agrobacterium-mediated
transformation of Arabidopsis thaliana. Plant
J 16:735–743
21. Verma SS, Chinnusamy V, Bansa KC (2008) A
simplified floral dip method for transformation
of Brassica napus and B. carinata. J Plant Biochem Biotechnol 17:197–200
22. Liu X, Brost J, Hutcheon C, Guilfoil R, Wilson
AK, Leung S et al (2012) Transformation of
the oilseed crop Camelina sativa by Agrobacterium-mediated floral dip and simple largescale screening of transformants. In Vitro Cell
Dev Biol 48:462–468
23. Lindsey BE III, Rivero L, Calhoun CS,
Grotewold E, Brkljacic J (2017) Standardized
method for high-throughput sterilization of
Arabidopsis seeds. J Vis Exp (128):e56587.
https://doi.org/10.3791/56587
24. Pischke MS, Huttlin EL, Hegeman AD, Sussman MR (2006) A transcriptome-based characterization of habituation in plant tissue
culture. Plant Physiol 140:1255–1278
25. Estelle MA, Somerville C (1987) Auxinresistant mutants of Arabidopsis thaliana with
altered morphology. Mol Gen Genet
206:200–206
Handling Arabidopsis Plants
23
Bot 54:993–1011
18. Koornneef M (1994) Arabidopsis genetics. In:
Meyerowitz E, Somerville C (eds) Arabidopsis.
Cold Spring Harbor Laboratory Press, Cold
Spring Harbor, pp 89–120
19. Nasrallah J (2011) Self-incompatibility in the
brassicaceae. In: Schmidt R, Bancroft I (eds)
Plant genetics and genomics: crops and models, genetics and genomics of the Brassicaceae,
vol 9. Springer, New York, pp 389–411
20. Clough SJ, Bent AF (1998) Floral dip: a simplified method for agrobacterium-mediated
transformation of Arabidopsis thaliana. Plant
J 16:735–743
21. Verma SS, Chinnusamy V, Bansa KC (2008) A
simplified floral dip method for transformation
of Brassica napus and B. carinata. J Plant Biochem Biotechnol 17:197–200
22. Liu X, Brost J, Hutcheon C, Guilfoil R, Wilson
AK, Leung S et al (2012) Transformation of
the oilseed crop Camelina sativa by Agrobacterium-mediated floral dip and simple largescale screening of transformants. In Vitro Cell
Dev Biol 48:462–468
23. Lindsey BE III, Rivero L, Calhoun CS,
Grotewold E, Brkljacic J (2017) Standardized
method for high-throughput sterilization of
Arabidopsis seeds. J Vis Exp (128):e56587.
https://doi.org/10.3791/56587
24. Pischke MS, Huttlin EL, Hegeman AD, Sussman MR (2006) A transcriptome-based characterization of habituation in plant tissue
culture. Plant Physiol 140:1255–1278
25. Estelle MA, Somerville C (1987) Auxinresistant mutants of Arabidopsis thaliana with
altered morphology. Mol Gen Genet
206:200–206
Handling Arabidopsis Plants
23
