the Arabidopsis root apex shown by highresolution cell-specific analysis of IAA distribution
and
synthesis.
Plant
Cell
21:1659–1668
19. Afonso CL, Harkins KR, Thomas-ComptonM, Krejci A, Galbraith DW (1985) Production of somatic hybrid plants through
fluorescence activated sorting of protoplasts.
Nat Biotechnol 3:811–816
20. Chattopadhyay PK, Perfetto SP, Yu J, Roederer M (2010) The use of quantum dot
nanocrystals in multicolor flow cytometry.
Wiley Interdiscip Rev Nanomed Nanobiotechnol 2:334–348
21. Nettey L, Giles AJ, Chattopadhyay PK (2018)
OMIP-050: a 28-color/30-parameter fluorescence flow cytometry panel to enumerate
and characterize cells expressing a wide array
of immune checkpoint molecules. Cytometry
A 93A:1094–1096
22. Chattopadhyay PK, Winters AF, Lomas WE
III, Laino AS, Woods DM (2019) Highparameter single-cell analysis. Annu Rev Anal
Chem 12:411–430
23. Galbraith DW, Mauch TJ (1980) Identification of fusion of plant protoplasts .2. Conditions for the reproducible fluorescence
labeling of protoplasts derived from mesophyll tissue. Z Pflanzenphysiol 98:129–140
24. Galbraith DW, Harkins KR, Jefferson RA
(1988) Flow cytometric characterization of
the chlorophyll contents and size distributions of plant protoplasts. Cytometry 9:75–83
25. Harkins KR, Jefferson RA, Kavanagh TA,
Bevan MW, Galbraith DW (1990) Expression
of photosynthesis-related gene fusions is
restricted by cell-type in transgenic plants
and in transfected protoplasts. Proc Natl
Acad Sci U S A 87:816–820
26. Crumpton-Taylor M, Grandison S, Png KM,
Bushby AJ, Smith AM (2012) Control of
starch granule numbers in Arabidopsis chloroplasts. Plant Physiol 158:905–916
27. Chalfie M, Tu Y, Euskirchen G, Ward WW,
Prasher DC (1994) Green fluorescent protein
as a marker for gene expression. Science
263:802–805
28. Haseloff J (1999) GFP variants for multispectral imaging of living cells. Methods Cell Biol
58:139–151
29. Snapp EL (2009) Fluorescent Proteins: a cell
biologist’s user guide. Trends Cell Biol
19:649–655
30. Berg RH, Beachy RN (2008) Fluorescent
protein applications in plants. Methods Cell
Biol 85:153–177
31. Ckurshumova W, Caragea AE, Goldstein RS,
Berleth T (2011) Glow in the dark: fluorescent proteins as cell and tissue-specific markers in plants. Mol Plant 4:794–804
32. Galbraith DW (2004) The rainbow of fluorescent proteins. Methods Cell Biol 75:153–169
33. Rizzo MA, Davidson MW, Piston DW (2009)
Fluorescent protein tracking and detection:
fluorescent protein structure and color variants. Cold Spring Harb Protoc 2009.
https://doi.org/10.1101/pdb.top63
34. Nelson BK, Cai X, Nebenfu ¨hr A (2007) A
multicolored set of in vivo organelle markers
for co-localization studies in Arabidopsis and
other plants. Plant J 51:1126–1136
35. Marque `s-Bueno MM, Morao AK, Cayrel A,
Platre MP, Barberon M, Caillieux E, Colot V,
Jaillais Y, Roudier F, Vert G (2016) A versatile
multisite gateway-compatible promoter and
transgenic line collection for cell type-specific
functional genomics in Arabidopsis. Plant J
85:320–333
36. Machin FQ, Beckers M, Tian X, Fairnie A,
Cheng T, Scheible WR, Doerner P (2019)
Inducible reporter/driver lines for the Arabidopsis root with intrinsic reporting of activity
state. Plant J 98:153–164
37. Haseloff J, Siemering KR, Prasher DC,
Hodge S (1997) Removal of a cryptic intron
and subcellular localization of green fluorescent protein are required to mark transgenic
Arabidopsis plants brightly. Proc Natl Acad
Sci U S A 94:2122–2127
38. Geldner N, De ´nervaud-Tendon V, Hyman
DL, Mayer U, Stierhof Y-D, Chory J (2009)
Rapid, combinatorial analysis of membrane
compartments in intact plants with a multicolor marker set. Plant J 59:169–178
39. Boisnard-Lorig C, Colon-Carmona A,
Bauch W, Hodge S, Doerner P, Bancharel E,
Dumas C, Haseloff J, Berger F (2001)
Dynamic analyses of the expression of the
HISTONE::YFP fusion protein in arabidopsis
show that syncytial endosperm is divided in
mitotic domains. Plant Cell 13:495–509
40. Cutler SR, Ehrhardt DW, Somerville CR
(2000) Random GFP::cDNA fusions enable
visualization of subcellular structures in cells
of Arabidopsis at a high frequency. Proc Natl
Acad Sci U S A 97:3718–3723
41. Grebenok RJ, Pierson EA, Lambert GM,
Gong F-C, Afonso CL, Haldeman-Cahill R,
Carrington JC, Galbraith DW (1997) Greenfluorescent protein fusions for efficient characterization of nuclear localization signals.
Plant J 11:573–586
Flow Cytometry and Sorting in Arabidopsis
289
and
synthesis.
Plant
Cell
21:1659–1668
19. Afonso CL, Harkins KR, Thomas-ComptonM, Krejci A, Galbraith DW (1985) Production of somatic hybrid plants through
fluorescence activated sorting of protoplasts.
Nat Biotechnol 3:811–816
20. Chattopadhyay PK, Perfetto SP, Yu J, Roederer M (2010) The use of quantum dot
nanocrystals in multicolor flow cytometry.
Wiley Interdiscip Rev Nanomed Nanobiotechnol 2:334–348
21. Nettey L, Giles AJ, Chattopadhyay PK (2018)
OMIP-050: a 28-color/30-parameter fluorescence flow cytometry panel to enumerate
and characterize cells expressing a wide array
of immune checkpoint molecules. Cytometry
A 93A:1094–1096
22. Chattopadhyay PK, Winters AF, Lomas WE
III, Laino AS, Woods DM (2019) Highparameter single-cell analysis. Annu Rev Anal
Chem 12:411–430
23. Galbraith DW, Mauch TJ (1980) Identification of fusion of plant protoplasts .2. Conditions for the reproducible fluorescence
labeling of protoplasts derived from mesophyll tissue. Z Pflanzenphysiol 98:129–140
24. Galbraith DW, Harkins KR, Jefferson RA
(1988) Flow cytometric characterization of
the chlorophyll contents and size distributions of plant protoplasts. Cytometry 9:75–83
25. Harkins KR, Jefferson RA, Kavanagh TA,
Bevan MW, Galbraith DW (1990) Expression
of photosynthesis-related gene fusions is
restricted by cell-type in transgenic plants
and in transfected protoplasts. Proc Natl
Acad Sci U S A 87:816–820
26. Crumpton-Taylor M, Grandison S, Png KM,
Bushby AJ, Smith AM (2012) Control of
starch granule numbers in Arabidopsis chloroplasts. Plant Physiol 158:905–916
27. Chalfie M, Tu Y, Euskirchen G, Ward WW,
Prasher DC (1994) Green fluorescent protein
as a marker for gene expression. Science
263:802–805
28. Haseloff J (1999) GFP variants for multispectral imaging of living cells. Methods Cell Biol
58:139–151
29. Snapp EL (2009) Fluorescent Proteins: a cell
biologist’s user guide. Trends Cell Biol
19:649–655
30. Berg RH, Beachy RN (2008) Fluorescent
protein applications in plants. Methods Cell
Biol 85:153–177
31. Ckurshumova W, Caragea AE, Goldstein RS,
Berleth T (2011) Glow in the dark: fluorescent proteins as cell and tissue-specific markers in plants. Mol Plant 4:794–804
32. Galbraith DW (2004) The rainbow of fluorescent proteins. Methods Cell Biol 75:153–169
33. Rizzo MA, Davidson MW, Piston DW (2009)
Fluorescent protein tracking and detection:
fluorescent protein structure and color variants. Cold Spring Harb Protoc 2009.
https://doi.org/10.1101/pdb.top63
34. Nelson BK, Cai X, Nebenfu ¨hr A (2007) A
multicolored set of in vivo organelle markers
for co-localization studies in Arabidopsis and
other plants. Plant J 51:1126–1136
35. Marque `s-Bueno MM, Morao AK, Cayrel A,
Platre MP, Barberon M, Caillieux E, Colot V,
Jaillais Y, Roudier F, Vert G (2016) A versatile
multisite gateway-compatible promoter and
transgenic line collection for cell type-specific
functional genomics in Arabidopsis. Plant J
85:320–333
36. Machin FQ, Beckers M, Tian X, Fairnie A,
Cheng T, Scheible WR, Doerner P (2019)
Inducible reporter/driver lines for the Arabidopsis root with intrinsic reporting of activity
state. Plant J 98:153–164
37. Haseloff J, Siemering KR, Prasher DC,
Hodge S (1997) Removal of a cryptic intron
and subcellular localization of green fluorescent protein are required to mark transgenic
Arabidopsis plants brightly. Proc Natl Acad
Sci U S A 94:2122–2127
38. Geldner N, De ´nervaud-Tendon V, Hyman
DL, Mayer U, Stierhof Y-D, Chory J (2009)
Rapid, combinatorial analysis of membrane
compartments in intact plants with a multicolor marker set. Plant J 59:169–178
39. Boisnard-Lorig C, Colon-Carmona A,
Bauch W, Hodge S, Doerner P, Bancharel E,
Dumas C, Haseloff J, Berger F (2001)
Dynamic analyses of the expression of the
HISTONE::YFP fusion protein in arabidopsis
show that syncytial endosperm is divided in
mitotic domains. Plant Cell 13:495–509
40. Cutler SR, Ehrhardt DW, Somerville CR
(2000) Random GFP::cDNA fusions enable
visualization of subcellular structures in cells
of Arabidopsis at a high frequency. Proc Natl
Acad Sci U S A 97:3718–3723
41. Grebenok RJ, Pierson EA, Lambert GM,
Gong F-C, Afonso CL, Haldeman-Cahill R,
Carrington JC, Galbraith DW (1997) Greenfluorescent protein fusions for efficient characterization of nuclear localization signals.
Plant J 11:573–586
Flow Cytometry and Sorting in Arabidopsis
289