program, and the National Institutes of Health. DG also acknowledges on-going support from the USDA through the University of
Arizona College of Agriculture and Life Sciences. We also acknowledge support from the Chinese National Key Research and Development Program (2OI6YFDOl0l006), the National Natural
Science Foundation of China (31770300), the Program for Innovative Research Teams in Science and Technology at a University of
Henan Province (18IRTSTHNO23), and the 111 Project
(D16014) of China. We finally thank Lisa Villalobos-Menuey
(Beckman-Coulter), Laurie Appling (SONY), and Matt Alexander
and Alex Rodriguez (BioRad), for valuable assistance in generating
the data of Figs. 2, 3, and 4.
References
1. Galbraith DW (2014) Flow cytometry and
sorting in Arabidopsis. In: Sanchez Serrano
JJ, Salinas J (eds) Arabidopsis Protocols, 3rd
ed. Methods in molecular biology, vol 1062.
Humana Press, Totowa, pp 509–537
2. Becker JD, Boavida LC, Carneiro J, Haury M,
Feijo ´ JA (2003) Transcriptional profiling of
Arabidopsis tissues reveals the unique characteristics of the pollen transcriptome. Plant
Physiol 133:713–725
3. Pina C, Pinto F, Feijo ´ JA, Becker JD (2005)
Gene family analysis of the Arabidopsis pollen
transcriptome reveals biological implications
for cell growth, division control, and gene
expression
regulation.
Plant
Physiol
138:744–756
4. Borges F, Gomes G, Gardner R, Moreno N,
McCormick S, Feijo ´ JA, Becker JD (2008)
Comparative transcriptomics of Arabidopsis
sperm cells. Plant Physiol 148:1168–1181
5. Luria G, Rutley N, Lazar I, Harper JF, Miller
G (2019) Direct analysis of pollen fitness by
flow cytometry: implications for pollen
response to stress. Plant J 98:942–952
6. Davey MR, Anthony P, Power JB, Lowe KC
(2005) Plant protoplasts: status and biotechnological perspectives. Biotechnol Adv
23:131–171
7. Galbraith DW, Harkins KR, Maddox JR,
Ayres NM, Sharma DP, Firoozabady E
(1983) Rapid flow cytometric analysis of the
cell cycle in intact plant tissues. Science
220:1049–1051
8. Harkins KR, Galbraith DW (1984) Flow sorting and culture of plant protoplasts. Physiol
Plant 60:43–52
9. Galbraith DW (1990) Isolation and flow cytometric characterization of plant protoplasts.
Methods Cell Biol 33:527–547
10. Galbraith DW, Bartos J, Dolezel J (2005) In:
Sklar LA (ed) Flow cytometry and cell sorting
in plant biotechnology. Oxford University
Press, New York, pp 291–322
11. Galbraith DW, Grebenok RJ, Lambert GM,
Sheen J (1995) Flow cytometric analysis of
transgene expression in higher plants: green
fluorescent protein. Methods Cell Biol
50:3–12
12. Sheen J, Hwang S, Niwa Y, Kobayashi H,
Galbraith DW (1995) Green fluorescent protein as a new vital marker in plant cells. Plant J
8:777–784
13. Galbraith DW, Herzenberg LA, Anderson M
(1999) Flow cytometric analysis of transgene
expression in higher plants: green fluorescent
protein. Methods Enzymol 320:296–315
14. Birnbaum K, Shasha DE, Wang JY, Jung JW,
Lambert GM, Galbraith DW, Benfey PN
(2003) A gene expression map of the Arabidopsis root. Science 302:1956–1960
15. Birnbaum K, Jung JW, Wang JY, Lambert
GM, Hirst JA, Galbraith DW, Benfey PN
(2005) Cell-type specific expression profiling
in plants using fluorescent reporter lines, protoplasting, and cell sorting. Nat Methods
2:1–5
16. Yadav RK, Girke T, Pasala S, Xie MT, Reddy V
(2009) Gene expression map of the Arabidopsis shoot apical meristem stem cell niche. Proc
Natl Acad Sci U S A 106:4941–4946
17. Yoo SD, Cho YH, Sheen J (2007) Arabidopsis
mesophyll protoplasts: a versatile cell system
for transient gene expression analysis. Nat
Protoc 2:1565–1572
18. Petersson SV, Johansson AI, Kowalczyk M,
Makoveychuk A, Wang JY, Moritz T,
Grebe M, Benfey PN, Sandberg G, Ljung K
(2009) An auxin gradient and maximum in
288
David W. Galbraith and Guiling Sun
Arizona College of Agriculture and Life Sciences. We also acknowledge support from the Chinese National Key Research and Development Program (2OI6YFDOl0l006), the National Natural
Science Foundation of China (31770300), the Program for Innovative Research Teams in Science and Technology at a University of
Henan Province (18IRTSTHNO23), and the 111 Project
(D16014) of China. We finally thank Lisa Villalobos-Menuey
(Beckman-Coulter), Laurie Appling (SONY), and Matt Alexander
and Alex Rodriguez (BioRad), for valuable assistance in generating
the data of Figs. 2, 3, and 4.
References
1. Galbraith DW (2014) Flow cytometry and
sorting in Arabidopsis. In: Sanchez Serrano
JJ, Salinas J (eds) Arabidopsis Protocols, 3rd
ed. Methods in molecular biology, vol 1062.
Humana Press, Totowa, pp 509–537
2. Becker JD, Boavida LC, Carneiro J, Haury M,
Feijo ´ JA (2003) Transcriptional profiling of
Arabidopsis tissues reveals the unique characteristics of the pollen transcriptome. Plant
Physiol 133:713–725
3. Pina C, Pinto F, Feijo ´ JA, Becker JD (2005)
Gene family analysis of the Arabidopsis pollen
transcriptome reveals biological implications
for cell growth, division control, and gene
expression
regulation.
Plant
Physiol
138:744–756
4. Borges F, Gomes G, Gardner R, Moreno N,
McCormick S, Feijo ´ JA, Becker JD (2008)
Comparative transcriptomics of Arabidopsis
sperm cells. Plant Physiol 148:1168–1181
5. Luria G, Rutley N, Lazar I, Harper JF, Miller
G (2019) Direct analysis of pollen fitness by
flow cytometry: implications for pollen
response to stress. Plant J 98:942–952
6. Davey MR, Anthony P, Power JB, Lowe KC
(2005) Plant protoplasts: status and biotechnological perspectives. Biotechnol Adv
23:131–171
7. Galbraith DW, Harkins KR, Maddox JR,
Ayres NM, Sharma DP, Firoozabady E
(1983) Rapid flow cytometric analysis of the
cell cycle in intact plant tissues. Science
220:1049–1051
8. Harkins KR, Galbraith DW (1984) Flow sorting and culture of plant protoplasts. Physiol
Plant 60:43–52
9. Galbraith DW (1990) Isolation and flow cytometric characterization of plant protoplasts.
Methods Cell Biol 33:527–547
10. Galbraith DW, Bartos J, Dolezel J (2005) In:
Sklar LA (ed) Flow cytometry and cell sorting
in plant biotechnology. Oxford University
Press, New York, pp 291–322
11. Galbraith DW, Grebenok RJ, Lambert GM,
Sheen J (1995) Flow cytometric analysis of
transgene expression in higher plants: green
fluorescent protein. Methods Cell Biol
50:3–12
12. Sheen J, Hwang S, Niwa Y, Kobayashi H,
Galbraith DW (1995) Green fluorescent protein as a new vital marker in plant cells. Plant J
8:777–784
13. Galbraith DW, Herzenberg LA, Anderson M
(1999) Flow cytometric analysis of transgene
expression in higher plants: green fluorescent
protein. Methods Enzymol 320:296–315
14. Birnbaum K, Shasha DE, Wang JY, Jung JW,
Lambert GM, Galbraith DW, Benfey PN
(2003) A gene expression map of the Arabidopsis root. Science 302:1956–1960
15. Birnbaum K, Jung JW, Wang JY, Lambert
GM, Hirst JA, Galbraith DW, Benfey PN
(2005) Cell-type specific expression profiling
in plants using fluorescent reporter lines, protoplasting, and cell sorting. Nat Methods
2:1–5
16. Yadav RK, Girke T, Pasala S, Xie MT, Reddy V
(2009) Gene expression map of the Arabidopsis shoot apical meristem stem cell niche. Proc
Natl Acad Sci U S A 106:4941–4946
17. Yoo SD, Cho YH, Sheen J (2007) Arabidopsis
mesophyll protoplasts: a versatile cell system
for transient gene expression analysis. Nat
Protoc 2:1565–1572
18. Petersson SV, Johansson AI, Kowalczyk M,
Makoveychuk A, Wang JY, Moritz T,
Grebe M, Benfey PN, Sandberg G, Ljung K
(2009) An auxin gradient and maximum in
288
David W. Galbraith and Guiling Sun