4.3.7 Effect of 2,4,6-Trinitrotoluene (TNT)
The LE of WRF was dependent on medium composition (Kachlishvili et al. 2016).
They have tested five WRF cultures, from that Cerena unicolor 300 produced the
highest yields upon growth either on glycerol medium or mandarin peel treated with
2,4,6-trinitrotoluene (TNT) at 0.2 mM. Yields of LMEs such as laccase and MnP on
mandarin peel + TNT were relatively higher than on glycerol + TNT. But, inhibition
of MnP production by Cerena unicolor 300 occurred within 5 days after exposure to
the highest concentration (0.4 mM) of TNT. This culture made recovery not only
from the toxicity of TNT at the increased concentration after 6 days of incubation but
also turned around in MnP production over control (Kachlishvili et al. 2016).
Fig. 4.10 (a) White rot fungi, (b) Growth of 6 days old culture of Stereum ostrea.(c1) Growth of
Stereum ostrea in the presence and absence of CPF under stationary. (c2) Growth of Stereum ostrea
on the tenth day of incubation in the presence and absence of CPF under shaking conditions. (d)
Identification of LAC enzyme from the culture filtrate of CPF-amended medium (color change of
the medium from colorless to dark red color indicates the presence of LAC enzyme)
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B. S. Shanthi Kumari et al.
The LE of WRF was dependent on medium composition (Kachlishvili et al. 2016).
They have tested five WRF cultures, from that Cerena unicolor 300 produced the
highest yields upon growth either on glycerol medium or mandarin peel treated with
2,4,6-trinitrotoluene (TNT) at 0.2 mM. Yields of LMEs such as laccase and MnP on
mandarin peel + TNT were relatively higher than on glycerol + TNT. But, inhibition
of MnP production by Cerena unicolor 300 occurred within 5 days after exposure to
the highest concentration (0.4 mM) of TNT. This culture made recovery not only
from the toxicity of TNT at the increased concentration after 6 days of incubation but
also turned around in MnP production over control (Kachlishvili et al. 2016).
Fig. 4.10 (a) White rot fungi, (b) Growth of 6 days old culture of Stereum ostrea.(c1) Growth of
Stereum ostrea in the presence and absence of CPF under stationary. (c2) Growth of Stereum ostrea
on the tenth day of incubation in the presence and absence of CPF under shaking conditions. (d)
Identification of LAC enzyme from the culture filtrate of CPF-amended medium (color change of
the medium from colorless to dark red color indicates the presence of LAC enzyme)
106
B. S. Shanthi Kumari et al.