100. Jiang X, Jiang Z, Xu T, Su S, Zhong Y, Peng F, Su Y, He Y (2013) Surface-enhanced
Raman scattering-based sensing in vitro: facile and label-free detection of apoptotic cells at
the single-cell level. Anal Chem 85:2809–2816
101. Pliss A, Kuzmin AN, Kachynski AV, Prasad PN (2010) Biophotonic probing of
macromolecular transformations during apoptosis. Proc Natl Acad Sci U S A 107:12771–
12776
102. Verrier S, Notingher I, Polak JM, Hench LL (2004) In situ monitoring of cell death using
Raman microspectroscopy. Biopolymers 74:157–162
103. Zoladek A, Pascut FC, Patel P, Notingher I (2011) Non-invasive time-course imaging of
apoptotic cells by confocal Raman micro-spectroscopy. J Raman Spectrosc 42:251–258
104. Fazio E, Trusso S, Franco D, Nicolò MS, Allegra A, Neri F, Musolino C, Guglielmino SP
(2016) A micro-Raman spectroscopic investigation of leukemic U-937 cells in aged cultures.
Spectrochim Acta A 159:21–29
105. Okada M, Smith NI, Palonpon AF, Endo H, Kawata S, Sodeoka M, Fujita K (2012)
Label-free Raman observation of cytochrome c dynamics during apoptosis. Proc Natl Acad
Sci 109:28–32
106. Czamara K, Petko F, Baranska M, Kaczor A (2016) Raman microscopy at the subcellular
level: a study on early apoptosis in endothelial cells induced by Fas ligand and
cycloheximide. Analyst 141:1390–1397
107. Hengartner MO (2000) The biochemistry of apoptosis. Nature 407:3–6
108. Schneider-Poetsch T, Ju J, Eyler DE, Dang Y, Bhat S, Merrick WC, Green R, Shen B,
Liu JO (2010) Inhibition of eukaryotic translation elongation by cycloheximide and
lactimidomycin. Nat Chem Biol 6:209–217
109. Farhane Z, Nawaz H, Bonnier F, Byrne HJ (2018) In vitro label-free screening of
chemotherapeutic drugs using Raman microspectroscopy: towards a new paradigm of
spectralomics. J Biophotonics 11. https://doi.org/10.1002/jbio.201700258
110. Siddique MR, Rutter AV, Wehbe K, Cinque G, Bellisolac G, Sulé-Suso J (2017) Effects of
nilotinib on leukaemia cells using vibrational microspectroscopy and cell cloning. Analyst
142:1299–1307
111. Nawaz H, Bonnier F, Meade AD, Lynga FM, Byrne HJ (2011) Comparison of subcellular
responses for the evaluation and prediction of the chemotherapeutic response to cisplatin in
lung adenocarcinoma using Raman spectroscopy. Analyst 136:2450–2463
112. Farhane Z, Bonnier F, Casey A, Byrne HJ (2015) Raman micro spectroscopy for in vitro
drug screening: subcellular localisation and interactions of doxorubicin. Analyst 140:4212–
4223
113. Gewirtz DA (1999) A critical evaluation of the mechanisms of action proposed for the
antitumor effects of the anthracycline antibiotics adriamycin and daunorubicin. Biochem
Pharmacol 57:727–741
114. Minotti G, Menna P, Salvatorelli E, Cairo G, Gianni L (2004) Anthracyclines: molecular
advances and pharmacologic developments in antitumor activity and cardiotoxicity.
Pharmacol Rev 56:185–229
115. Ciesielska E, Studzian K, Wąsowska M, Oszczapowicz I, Szmigiero L (2015) Cytotoxicity,
cellular uptake and DNA damage by daunorubicin and its new analogues with modified
daunosamine moiety. Cell Biol Toxicol 21:139–147
116. Szafraniec E, Majzner K, Farhane Z, Byrne HJ, Lukawska M, Oszczapowicz I, Chlopicki S,
Baranska M (2016) Spectroscopic studies of anthracyclines: structural characterization and
in vitro tracking. Spectrochim Acta A 169:152–160
117. Majzner K, Wojcik T, Szafraniec E, Lukawska M, Oszczapowicz I, Chlopicki S,
Baranska M (2015) Nuclear accumulation of anthracyclines in the endothelium studied by
bimodal imaging: fluorescence and Raman microscopy. Analyst 140:2302–2310
118. Zhang Q, Lu X, Tang P, Zhang D, Zhong L, Tian J (2016) Gold nanoparticle (AuNP)-based
surface-enhanced Raman scattering (SERS) probe of leukemic lymphocytes. Plasmonics
11:1361–1368
119. Fabris L (2015) Gold-based SERS tags for biomedical imaging. J Opt 17:114002
6 Small and Large Molecules Investigated by Raman Spectroscopy
193
Raman scattering-based sensing in vitro: facile and label-free detection of apoptotic cells at
the single-cell level. Anal Chem 85:2809–2816
101. Pliss A, Kuzmin AN, Kachynski AV, Prasad PN (2010) Biophotonic probing of
macromolecular transformations during apoptosis. Proc Natl Acad Sci U S A 107:12771–
12776
102. Verrier S, Notingher I, Polak JM, Hench LL (2004) In situ monitoring of cell death using
Raman microspectroscopy. Biopolymers 74:157–162
103. Zoladek A, Pascut FC, Patel P, Notingher I (2011) Non-invasive time-course imaging of
apoptotic cells by confocal Raman micro-spectroscopy. J Raman Spectrosc 42:251–258
104. Fazio E, Trusso S, Franco D, Nicolò MS, Allegra A, Neri F, Musolino C, Guglielmino SP
(2016) A micro-Raman spectroscopic investigation of leukemic U-937 cells in aged cultures.
Spectrochim Acta A 159:21–29
105. Okada M, Smith NI, Palonpon AF, Endo H, Kawata S, Sodeoka M, Fujita K (2012)
Label-free Raman observation of cytochrome c dynamics during apoptosis. Proc Natl Acad
Sci 109:28–32
106. Czamara K, Petko F, Baranska M, Kaczor A (2016) Raman microscopy at the subcellular
level: a study on early apoptosis in endothelial cells induced by Fas ligand and
cycloheximide. Analyst 141:1390–1397
107. Hengartner MO (2000) The biochemistry of apoptosis. Nature 407:3–6
108. Schneider-Poetsch T, Ju J, Eyler DE, Dang Y, Bhat S, Merrick WC, Green R, Shen B,
Liu JO (2010) Inhibition of eukaryotic translation elongation by cycloheximide and
lactimidomycin. Nat Chem Biol 6:209–217
109. Farhane Z, Nawaz H, Bonnier F, Byrne HJ (2018) In vitro label-free screening of
chemotherapeutic drugs using Raman microspectroscopy: towards a new paradigm of
spectralomics. J Biophotonics 11. https://doi.org/10.1002/jbio.201700258
110. Siddique MR, Rutter AV, Wehbe K, Cinque G, Bellisolac G, Sulé-Suso J (2017) Effects of
nilotinib on leukaemia cells using vibrational microspectroscopy and cell cloning. Analyst
142:1299–1307
111. Nawaz H, Bonnier F, Meade AD, Lynga FM, Byrne HJ (2011) Comparison of subcellular
responses for the evaluation and prediction of the chemotherapeutic response to cisplatin in
lung adenocarcinoma using Raman spectroscopy. Analyst 136:2450–2463
112. Farhane Z, Bonnier F, Casey A, Byrne HJ (2015) Raman micro spectroscopy for in vitro
drug screening: subcellular localisation and interactions of doxorubicin. Analyst 140:4212–
4223
113. Gewirtz DA (1999) A critical evaluation of the mechanisms of action proposed for the
antitumor effects of the anthracycline antibiotics adriamycin and daunorubicin. Biochem
Pharmacol 57:727–741
114. Minotti G, Menna P, Salvatorelli E, Cairo G, Gianni L (2004) Anthracyclines: molecular
advances and pharmacologic developments in antitumor activity and cardiotoxicity.
Pharmacol Rev 56:185–229
115. Ciesielska E, Studzian K, Wąsowska M, Oszczapowicz I, Szmigiero L (2015) Cytotoxicity,
cellular uptake and DNA damage by daunorubicin and its new analogues with modified
daunosamine moiety. Cell Biol Toxicol 21:139–147
116. Szafraniec E, Majzner K, Farhane Z, Byrne HJ, Lukawska M, Oszczapowicz I, Chlopicki S,
Baranska M (2016) Spectroscopic studies of anthracyclines: structural characterization and
in vitro tracking. Spectrochim Acta A 169:152–160
117. Majzner K, Wojcik T, Szafraniec E, Lukawska M, Oszczapowicz I, Chlopicki S,
Baranska M (2015) Nuclear accumulation of anthracyclines in the endothelium studied by
bimodal imaging: fluorescence and Raman microscopy. Analyst 140:2302–2310
118. Zhang Q, Lu X, Tang P, Zhang D, Zhong L, Tian J (2016) Gold nanoparticle (AuNP)-based
surface-enhanced Raman scattering (SERS) probe of leukemic lymphocytes. Plasmonics
11:1361–1368
119. Fabris L (2015) Gold-based SERS tags for biomedical imaging. J Opt 17:114002
6 Small and Large Molecules Investigated by Raman Spectroscopy
193