80. Elsheikha HM, Alkurashi M, Kong K, Zhu X-Q (2014) Metabolic footprinting of
extracellular metabolites of brain endothelium infected with Neospora caninum in vitro.
BMC Res Notes 7:406
81. Große C, Bergner N, Dellith J, Heller R, Bauer M, Mellmann A, Popp J, Neugebauer U
(2015) Label-free imaging and spectroscopic analysis of intracellular bacterial infections.
Anal Chem 87:2137–2142
82. Kong K, Rowlands CJ, Elsheikha H, Notingher I (2012) Label-free molecular analysis of
live Neospora caninum tachyzoites in host cells by selective scanning Raman
micro-spectroscopy. Analyst 137:4119–4122
83. Majzner K, Kochan K, Kachamakova-Trojanowska N, Maslak E, Chlopicki S, Baranska M
(2014) Raman imaging provides insights into chemical composition of lipid droplets of
different size and origin: in hepatocytes and endothelium. Anal Chem 86:6666–6674
84. Majzner K, Chlopicki S, Baranska M (2016) Lipid droplets formation in human endothelial
cells in response to polyunsaturated fatty acids and 1-methyl-nicotinamide (MNA); confocal
Raman imaging and fluorescence microscopy studies. J Biophotonics 9:396–405
85. Astanina K, Koch M, Jüngst C, Zumbusch A, Kiemer AK (2015) Lipid droplets as a novel
cargo of tunnelling nanotubes in endothelial cells. Sci Rep 5:11453
86. Melo RC, D’Avila H, Wan HC, Bozza PT, Dvorak AM, Weller PF (2011) Lipid bodies in
inflammatory cells. J Histochem Cytochem 59:540–556
87. Pi J, Li T, Liu J, Su X, Wang R, Yang F, Bai H, Jin H, Cai J (2014) Detection of
lipopolysaccharide induced inflammatory responses in RAW264.7 macrophages using
atomic force microscope. Micron 65:1–9
88. Szymonski M, Targosz-Korecka M, Malek-Zietek KE (2015) Nano-mechanical model of
endothelial dysfunction for AFM-based diagnostics at the cellular level. Pharmacol Rep
67:728–735
89. Pober JS, Sessa WC (2007) Evolving functions of endothelial cells in inflammation. Nat Rev
Immunol 7:803–815
90. Czamara K, Majzner K, Selmi A, Baranska M, Ozaki Y, Kaczor A (2017) Unsaturated lipid
bodies as a hallmark of inflammation studied by Raman 2D and 3D microscopy. Sci Rep
7:40889
91. Zhang D, Feng Y, Zhang Q, Su X, Lu X, Liu S, Zhong L (2015) Raman spectrum reveals the
cell cycle arrest of Triptolide-induced leukemic T-lymphocytes apoptosis. Spectrochim
Acta A 141:216–222
92. Moritz TJ, Taylor DS, Krol DM, Fritch J, Chan JW (2010) Detection of doxorubicininduced apoptosis of leukemic T-lymphocytes by laser tweezers Raman spectroscopy.
Biomed Opt Express 1:1138–1147
93. Huang H, Shi H, Feng S, Chen W, Yu Y, Lina D, Chen R (2013) Confocal Raman
spectroscopic analysis of the cytotoxic response to cisplatin in nasopharyngeal carcinoma
cells. Anal Methods 5:260–266
94. Lipiec E, Bambery KR, Heraud P, Kwiatek WM, McNaughton D, Tobin MJ, Vogel C,
Wood BR (2014) Monitoring UVR induced damage in single cells and isolated nuclei using
SR-FTIR microspectroscopy and 3D confocal Raman imaging. Analyst 139:4200–4209
95. Li B, Lu MQ, Wang QZ, Shi G, Liao W, Huang S (2015) Raman spectra analysis for single
mitochondrias after apoptosis process of yeast cells stressed by acetic acid. Fenxi Huaxue
Chin J Anal Chem 43:643–650
96. Yao H, Tao Z, Ai M, Peng L, Wang G, He B, Li Y (2009) Raman spectroscopic analysis of
apoptosis of single human gastric cancer cells. Vib Spectrosc 50:193–197
97. Panza J, Maier J (2007) Raman spectroscopy and Raman chemical imaging of apoptotic
cells. Imag Manip Anal Biomol Cells Tissue V 6441:6441081–64410812
98. Ong YH, Lim M, Liu Q (2012) Comparison of principal component analysis and
biochemical component analysis in Raman spectroscopy for the discrimination of apoptosis
and necrosis in K562 leukemia cells. Opt Express 20:22158–22171
99. Brauchle E, Thude S, Brucker SY, Schenke-Layland K (2015) Cell death stages in single
apoptotic and necrotic cells monitored by Raman microspectroscopy. Sci Rep 4:4698
192
K. Czamara et al.
extracellular metabolites of brain endothelium infected with Neospora caninum in vitro.
BMC Res Notes 7:406
81. Große C, Bergner N, Dellith J, Heller R, Bauer M, Mellmann A, Popp J, Neugebauer U
(2015) Label-free imaging and spectroscopic analysis of intracellular bacterial infections.
Anal Chem 87:2137–2142
82. Kong K, Rowlands CJ, Elsheikha H, Notingher I (2012) Label-free molecular analysis of
live Neospora caninum tachyzoites in host cells by selective scanning Raman
micro-spectroscopy. Analyst 137:4119–4122
83. Majzner K, Kochan K, Kachamakova-Trojanowska N, Maslak E, Chlopicki S, Baranska M
(2014) Raman imaging provides insights into chemical composition of lipid droplets of
different size and origin: in hepatocytes and endothelium. Anal Chem 86:6666–6674
84. Majzner K, Chlopicki S, Baranska M (2016) Lipid droplets formation in human endothelial
cells in response to polyunsaturated fatty acids and 1-methyl-nicotinamide (MNA); confocal
Raman imaging and fluorescence microscopy studies. J Biophotonics 9:396–405
85. Astanina K, Koch M, Jüngst C, Zumbusch A, Kiemer AK (2015) Lipid droplets as a novel
cargo of tunnelling nanotubes in endothelial cells. Sci Rep 5:11453
86. Melo RC, D’Avila H, Wan HC, Bozza PT, Dvorak AM, Weller PF (2011) Lipid bodies in
inflammatory cells. J Histochem Cytochem 59:540–556
87. Pi J, Li T, Liu J, Su X, Wang R, Yang F, Bai H, Jin H, Cai J (2014) Detection of
lipopolysaccharide induced inflammatory responses in RAW264.7 macrophages using
atomic force microscope. Micron 65:1–9
88. Szymonski M, Targosz-Korecka M, Malek-Zietek KE (2015) Nano-mechanical model of
endothelial dysfunction for AFM-based diagnostics at the cellular level. Pharmacol Rep
67:728–735
89. Pober JS, Sessa WC (2007) Evolving functions of endothelial cells in inflammation. Nat Rev
Immunol 7:803–815
90. Czamara K, Majzner K, Selmi A, Baranska M, Ozaki Y, Kaczor A (2017) Unsaturated lipid
bodies as a hallmark of inflammation studied by Raman 2D and 3D microscopy. Sci Rep
7:40889
91. Zhang D, Feng Y, Zhang Q, Su X, Lu X, Liu S, Zhong L (2015) Raman spectrum reveals the
cell cycle arrest of Triptolide-induced leukemic T-lymphocytes apoptosis. Spectrochim
Acta A 141:216–222
92. Moritz TJ, Taylor DS, Krol DM, Fritch J, Chan JW (2010) Detection of doxorubicininduced apoptosis of leukemic T-lymphocytes by laser tweezers Raman spectroscopy.
Biomed Opt Express 1:1138–1147
93. Huang H, Shi H, Feng S, Chen W, Yu Y, Lina D, Chen R (2013) Confocal Raman
spectroscopic analysis of the cytotoxic response to cisplatin in nasopharyngeal carcinoma
cells. Anal Methods 5:260–266
94. Lipiec E, Bambery KR, Heraud P, Kwiatek WM, McNaughton D, Tobin MJ, Vogel C,
Wood BR (2014) Monitoring UVR induced damage in single cells and isolated nuclei using
SR-FTIR microspectroscopy and 3D confocal Raman imaging. Analyst 139:4200–4209
95. Li B, Lu MQ, Wang QZ, Shi G, Liao W, Huang S (2015) Raman spectra analysis for single
mitochondrias after apoptosis process of yeast cells stressed by acetic acid. Fenxi Huaxue
Chin J Anal Chem 43:643–650
96. Yao H, Tao Z, Ai M, Peng L, Wang G, He B, Li Y (2009) Raman spectroscopic analysis of
apoptosis of single human gastric cancer cells. Vib Spectrosc 50:193–197
97. Panza J, Maier J (2007) Raman spectroscopy and Raman chemical imaging of apoptotic
cells. Imag Manip Anal Biomol Cells Tissue V 6441:6441081–64410812
98. Ong YH, Lim M, Liu Q (2012) Comparison of principal component analysis and
biochemical component analysis in Raman spectroscopy for the discrimination of apoptosis
and necrosis in K562 leukemia cells. Opt Express 20:22158–22171
99. Brauchle E, Thude S, Brucker SY, Schenke-Layland K (2015) Cell death stages in single
apoptotic and necrotic cells monitored by Raman microspectroscopy. Sci Rep 4:4698
192
K. Czamara et al.