PART VI BIOTECHNOLOGY
23 Use of Potyvirus Vectors to Produce Carotenoids in Plants . . . . . . . . . . . . . . . . . . 303
Jose ´-Antonio Daro `s
24 Agrobacterium tumefaciens-Mediated Stable Transformation
of Daucus carota. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 313
Christian Gonzalez-Calquin and Claudia Stange
25 CRISPR-Cas-Mediated Gene Knockout in Tomato . . . . . . . . . . . . . . . . . . . . . . . . . 321
Gwen Swinnen, Thomas Jacobs, Laurens Pauwels, and Alain Goossens
26 Modulation of Activity of a Carotenoid Pathway Through the
Use of the TeT-on Regulatory System: Application in the Fungus
Fusarium fujikuroi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 343
Julia Marente, Pedro Ortega, Javier Pardo-Medina, Javier Avalos,
and M. Carmen Lim on
PART VII NUTRITION AND HEALTH
27 Intervention Studies in Humans . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 363
Begon ˜a Olmedilla-Alonso
28 In Vitro Digestion for Control and Monitoring of Food Effects
in Relation to Micellarization Index of Carotenoids . . . . . . . . . . . . . . . . . . . . . . . . . 375
Antonio Pe ´rez-Ga ´ lvez and Javier Fontecha
29 Evaluation of Carotenoids Protection Against Oxidative Stress
in the Animal Model Caenorhabditis elegans. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 387
Patricia Martorell, Silvia Llopis, Jose ´ Vicente Gil, Salvador Genove ´s,
Daniel Ram on, Lorenzo Zacarı ´as, and Marı ´a Jesu ´ s Rodrigo
30 Mouse Models to Study Antiobesogenic Effects of Carotenoids . . . . . . . . . . . . . . 403
Joan Ribot, Bojan Stojnic, Andreu Palou, and M. Luisa Bonet
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 419
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