10 Acquisition of Mass Spectrometry Data of Carotenoids: A Focus
on Big Data Management. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
Antonio Pe ´rez-Ga ´ lvez, Isabel Viera, and Marı ´a Roca
11 cis/trans Carotenoid Extraction, Purification, Detection,
Quantification, and Profiling in Plant Tissues. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Yagiz Alagoz, Namraj Dhami, Chris Mitchell,
and Christopher I. Cazzonelli
12 Determination of Plant Volatile Apocarotenoids . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165
Jose ´ L. Rambla and Antonio Granell
13 A Method for Extraction and LC-MS-Based Identification
of Carotenoid-Derived Dialdehydes in Plants. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177
Jianing Mi, Kun-Peng Jia, Aparna Balakrishna,
and Salim Al-Babili
14 Fractionation of Tomato Fruit Chromoplasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 189
Karel De Pourcq and Albert Boronat
15 Quantification of Strigolactones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 199
Carlos Rial, Rosa M. Varela, Jose ´ M. G. Molinillo,
Alexandra G. Dura ´ n, and Francisco A. Macı ´as
16 Carotenoid and Apocarotenoid Analysis by SFE-SFC-QqQ/MS. . . . . . . . . . . . . . 209
Daniele Giuffrida, Mariosimone Zoccali, and Luigi Mondello
PART IV IMAGING
17 Elucidating Carotenoid Biosynthetic Enzyme Localization
and Interactions Using Fluorescent Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . 223
Maria Shumskaya, Rena F. Quinlan, and Eleanore T. Wurtzel
18 Visualization of Carotenoid-Storage Structures in Fruits
by Transmission Electron Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 235
Joanna Lado, Jaime Zacarias, Marı ´a Jesu ´ s Rodrigo,
and Lorenzo Zacarı ´as
19 Light Microscopy and Raman Imaging of Carotenoids in
Plant Cells In Situ and in Released Carotene Crystals . . . . . . . . . . . . . . . . . . . . . . . 245
Tomasz Oleszkiewicz, Marta Z. Pacia, Ewa Grzebelus,
and Rafal Baranski
PART V DYNAMICS
20 Carotenoid Isotopolog Profiling in
13 C-Labeled Leaf Extracts
by LC-MS and LC-FTICR-MS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 263
Bjo ¨rn Thiele and Shizue Matsubara
21 Quantification of Carotenoid Pathway Flux in Green and
Nongreen Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279
Julian Koschmieder and Ralf Welsch
22 Histochemical and Molecular Quantification of Arbuscular
Mycorrhiza Symbiosis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 293
Juan M. Garcı ´a, Marı ´a J. Pozo, and Juan A. L opez-Ra ´ ez
viii
Contents
on Big Data Management. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
Antonio Pe ´rez-Ga ´ lvez, Isabel Viera, and Marı ´a Roca
11 cis/trans Carotenoid Extraction, Purification, Detection,
Quantification, and Profiling in Plant Tissues. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Yagiz Alagoz, Namraj Dhami, Chris Mitchell,
and Christopher I. Cazzonelli
12 Determination of Plant Volatile Apocarotenoids . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165
Jose ´ L. Rambla and Antonio Granell
13 A Method for Extraction and LC-MS-Based Identification
of Carotenoid-Derived Dialdehydes in Plants. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177
Jianing Mi, Kun-Peng Jia, Aparna Balakrishna,
and Salim Al-Babili
14 Fractionation of Tomato Fruit Chromoplasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 189
Karel De Pourcq and Albert Boronat
15 Quantification of Strigolactones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 199
Carlos Rial, Rosa M. Varela, Jose ´ M. G. Molinillo,
Alexandra G. Dura ´ n, and Francisco A. Macı ´as
16 Carotenoid and Apocarotenoid Analysis by SFE-SFC-QqQ/MS. . . . . . . . . . . . . . 209
Daniele Giuffrida, Mariosimone Zoccali, and Luigi Mondello
PART IV IMAGING
17 Elucidating Carotenoid Biosynthetic Enzyme Localization
and Interactions Using Fluorescent Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . 223
Maria Shumskaya, Rena F. Quinlan, and Eleanore T. Wurtzel
18 Visualization of Carotenoid-Storage Structures in Fruits
by Transmission Electron Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 235
Joanna Lado, Jaime Zacarias, Marı ´a Jesu ´ s Rodrigo,
and Lorenzo Zacarı ´as
19 Light Microscopy and Raman Imaging of Carotenoids in
Plant Cells In Situ and in Released Carotene Crystals . . . . . . . . . . . . . . . . . . . . . . . 245
Tomasz Oleszkiewicz, Marta Z. Pacia, Ewa Grzebelus,
and Rafal Baranski
PART V DYNAMICS
20 Carotenoid Isotopolog Profiling in
13 C-Labeled Leaf Extracts
by LC-MS and LC-FTICR-MS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 263
Bjo ¨rn Thiele and Shizue Matsubara
21 Quantification of Carotenoid Pathway Flux in Green and
Nongreen Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279
Julian Koschmieder and Ralf Welsch
22 Histochemical and Molecular Quantification of Arbuscular
Mycorrhiza Symbiosis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 293
Juan M. Garcı ´a, Marı ´a J. Pozo, and Juan A. L opez-Ra ´ ez
viii
Contents