31. Rubio A, Rambla JL, Santaella M, Gomez MD,
Orzaez D, Granell A, Gomez-Gomez L (2008)
Cytosolic and plastoglobule-targeted carotenoid dioxygenases from Crocus sativus are both
involved in beta-ionone release. J Biol Chem
283(36):24816–24825..
M804000200.
https://doi.org/10.1074/jbc.M804000200
32. Huang FC, Horvath G, Molnar P, Turcsi E,
Deli J, Schrader J, Sandmann G, Schmidt H,
Schwab W (2009) Substrate promiscuity of
RdCCD1, a carotenoid cleavage oxygenase
from Rosa damascena. Phytochemistry 70
(4):457–464..
S0031-9422(09)00059-4.
https://doi.org/10.1016/j.phytochem.2009.
01.020
33. Simkin AJ, Underwood BA, Auldridge M,
Loucas HM, Shibuya K, Schmelz E, Clark
DG, Klee HJ (2004) Circadian regulation of
the PhCCD1 carotenoid cleavage dioxygenase
controls emission of beta-ionone, a fragrance
volatile of petunia flowers. Plant Physiol 136
(3):3504–3514.. 104.049718. https://doi.
org/10.1104/pp.104.049718
34. Simkin AJ, Schwartz SH, Auldridge M, Taylor
MG, Klee HJ (2004) The tomato carotenoid
cleavage dioxygenase 1 genes contribute to the
formation of the flavor volatiles beta-ionone,
pseudoionone, and geranylacetone. Plant J 40
(6):882–892. TPJ2263 [pii]. https://doi.org/
10.1111/j.1365-313X.2004.02263.x
35. Ilg A, Bruno M, Beyer P, Al-Babili S (2014)
Tomato carotenoid cleavage dioxygenases 1A
and 1B: relaxed double bond specificity leads to
a
plenitude
of
dialdehydes,
monoapocarotenoids and isoprenoid volatiles. FEBS
Open Bio 4:584–593. https://doi.org/10.
1016/j.fob.2014.06.005S2211-5463(14)
00060-6. [pii]
36. Ilg A, Beyer P, Al-Babili S (2009) Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial
biosynthesis. FEBS J 276(3):736–747.
EJB6820 [pii]. https://doi.org/10.1111/j.
1742-4658.2008.06820.x
37. Ibdah M, Azulay Y, Portnoy V, Wasserman B,
Bar E, Meir A, Burger Y, Hirschberg J, Schaffer
AA, Katzir N, Tadmor Y, Lewinsohn E (2006)
Functional characterization of CmCCD1, a
carotenoid cleavage dioxygenase from melon.
Phytochemistry 67(15):1579–1589.. S00319422(06)00109-9.
https://doi.org/10.
1016/j.phytochem.2006.02.009
38. Vogel JT, Tan BC, McCarty DR, Klee HJ
(2008) The carotenoid cleavage dioxygenase
1 enzyme has broad substrate specificity, cleaving multiple carotenoids at two different bond
positions.
J
Biol
Chem
283
(17):11364–11373.. M710106200. https://
doi.org/10.1074/jbc.M710106200
39. Mathieu S, Terrier N, Procureur J, Bigey F,
Gunata Z (2005) A carotenoid cleavage dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry
development
in
relation
to
C13-norisoprenoid accumulation. J Exp Bot
56(420):2721–2731. eri265 [pii]. https://
doi.org/10.1093/jxb/eri265
40. Lashbrooke JG, Young PR, Dockrall SJ,
Vasanth K, Vivier MA (2013) Functional characterisation of three members of the Vitis vinifera L. carotenoid cleavage dioxygenase gene
family. BMC Plant Biol 13:156. https://doi.
org/10.1186/1471-2229-13-15614712229-13-156. [pii]
41. Ma G, Zhang L, Matsuta A, Matsutani K,
Yamawaki K, Yahata M, Wahyudi A,
Motohashi R, Kato M (2013) Enzymatic formation of beta-Citraurin from betaCryptoxanthin and Zeaxanthin by carotenoid
cleavage Dioxygenase4 in the Flavedo of Citrus
fruit. Plant Physiol 163(2):682–695. https://
doi.org/10.1104/pp.113.223297.
pp.113.223297 [pii]
42. Huang FC, Molnar P, Schwab W (2009) Cloning and functional characterization of carotenoid cleavage dioxygenase 4 genes. J Exp Bot 60
(11):3011–3022.. erp137 [pii]. https://doi.
org/10.1093/jxb/erp137
43. Schwartz SH, Qin X, Zeevaart JA (2001) Characterization of a novel carotenoid cleavage
dioxygenase from plants. J Biol Chem 276
(27):25208–25211.
https://doi.org/10.
1074/jbc.M102146200. [pii]
44. Schwartz SH, Qin X, Loewen MC (2004) The
biochemical characterization of two carotenoid
cleavage enzymes from Arabidopsis indicates
that a carotenoid-derived compound inhibits
lateral branching. J Biol Chem 279
(45):46940–46945.
https://doi.org/10.
1074/jbc.M409004200. [pii]
45. Booker J, Auldridge M, Wills S, McCarty D,
Klee H, Leyser C (2004) MAX3/CCD7 is a
carotenoid cleavage dioxygenase required for
the synthesis of a novel plant signaling molecule. Curr Biol 14:7
46. Alder A, Holdermann I, Beyer P, Al-Babili S
(2008) Carotenoid oxygenases involved in
plant branching catalyse a highly specific conserved apocarotenoid cleavage reaction. Biochem J 416(2):289–296.. BJ20080568.
https://doi.org/10.1042/BJ20080568
47. Sergeant MJ, Li JJ, Fox C, Brookbank N,
Rea D, Bugg TD, Thompson AJ (2009) Selective inhibition of carotenoid cleavage dioxygenases: phenotypic effects on shoot
Determination of In Vitro and In Vivo Activities of Plant Carotenoid. . .
73
Orzaez D, Granell A, Gomez-Gomez L (2008)
Cytosolic and plastoglobule-targeted carotenoid dioxygenases from Crocus sativus are both
involved in beta-ionone release. J Biol Chem
283(36):24816–24825..
M804000200.
https://doi.org/10.1074/jbc.M804000200
32. Huang FC, Horvath G, Molnar P, Turcsi E,
Deli J, Schrader J, Sandmann G, Schmidt H,
Schwab W (2009) Substrate promiscuity of
RdCCD1, a carotenoid cleavage oxygenase
from Rosa damascena. Phytochemistry 70
(4):457–464..
S0031-9422(09)00059-4.
https://doi.org/10.1016/j.phytochem.2009.
01.020
33. Simkin AJ, Underwood BA, Auldridge M,
Loucas HM, Shibuya K, Schmelz E, Clark
DG, Klee HJ (2004) Circadian regulation of
the PhCCD1 carotenoid cleavage dioxygenase
controls emission of beta-ionone, a fragrance
volatile of petunia flowers. Plant Physiol 136
(3):3504–3514.. 104.049718. https://doi.
org/10.1104/pp.104.049718
34. Simkin AJ, Schwartz SH, Auldridge M, Taylor
MG, Klee HJ (2004) The tomato carotenoid
cleavage dioxygenase 1 genes contribute to the
formation of the flavor volatiles beta-ionone,
pseudoionone, and geranylacetone. Plant J 40
(6):882–892. TPJ2263 [pii]. https://doi.org/
10.1111/j.1365-313X.2004.02263.x
35. Ilg A, Bruno M, Beyer P, Al-Babili S (2014)
Tomato carotenoid cleavage dioxygenases 1A
and 1B: relaxed double bond specificity leads to
a
plenitude
of
dialdehydes,
monoapocarotenoids and isoprenoid volatiles. FEBS
Open Bio 4:584–593. https://doi.org/10.
1016/j.fob.2014.06.005S2211-5463(14)
00060-6. [pii]
36. Ilg A, Beyer P, Al-Babili S (2009) Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial
biosynthesis. FEBS J 276(3):736–747.
EJB6820 [pii]. https://doi.org/10.1111/j.
1742-4658.2008.06820.x
37. Ibdah M, Azulay Y, Portnoy V, Wasserman B,
Bar E, Meir A, Burger Y, Hirschberg J, Schaffer
AA, Katzir N, Tadmor Y, Lewinsohn E (2006)
Functional characterization of CmCCD1, a
carotenoid cleavage dioxygenase from melon.
Phytochemistry 67(15):1579–1589.. S00319422(06)00109-9.
https://doi.org/10.
1016/j.phytochem.2006.02.009
38. Vogel JT, Tan BC, McCarty DR, Klee HJ
(2008) The carotenoid cleavage dioxygenase
1 enzyme has broad substrate specificity, cleaving multiple carotenoids at two different bond
positions.
J
Biol
Chem
283
(17):11364–11373.. M710106200. https://
doi.org/10.1074/jbc.M710106200
39. Mathieu S, Terrier N, Procureur J, Bigey F,
Gunata Z (2005) A carotenoid cleavage dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry
development
in
relation
to
C13-norisoprenoid accumulation. J Exp Bot
56(420):2721–2731. eri265 [pii]. https://
doi.org/10.1093/jxb/eri265
40. Lashbrooke JG, Young PR, Dockrall SJ,
Vasanth K, Vivier MA (2013) Functional characterisation of three members of the Vitis vinifera L. carotenoid cleavage dioxygenase gene
family. BMC Plant Biol 13:156. https://doi.
org/10.1186/1471-2229-13-15614712229-13-156. [pii]
41. Ma G, Zhang L, Matsuta A, Matsutani K,
Yamawaki K, Yahata M, Wahyudi A,
Motohashi R, Kato M (2013) Enzymatic formation of beta-Citraurin from betaCryptoxanthin and Zeaxanthin by carotenoid
cleavage Dioxygenase4 in the Flavedo of Citrus
fruit. Plant Physiol 163(2):682–695. https://
doi.org/10.1104/pp.113.223297.
pp.113.223297 [pii]
42. Huang FC, Molnar P, Schwab W (2009) Cloning and functional characterization of carotenoid cleavage dioxygenase 4 genes. J Exp Bot 60
(11):3011–3022.. erp137 [pii]. https://doi.
org/10.1093/jxb/erp137
43. Schwartz SH, Qin X, Zeevaart JA (2001) Characterization of a novel carotenoid cleavage
dioxygenase from plants. J Biol Chem 276
(27):25208–25211.
https://doi.org/10.
1074/jbc.M102146200. [pii]
44. Schwartz SH, Qin X, Loewen MC (2004) The
biochemical characterization of two carotenoid
cleavage enzymes from Arabidopsis indicates
that a carotenoid-derived compound inhibits
lateral branching. J Biol Chem 279
(45):46940–46945.
https://doi.org/10.
1074/jbc.M409004200. [pii]
45. Booker J, Auldridge M, Wills S, McCarty D,
Klee H, Leyser C (2004) MAX3/CCD7 is a
carotenoid cleavage dioxygenase required for
the synthesis of a novel plant signaling molecule. Curr Biol 14:7
46. Alder A, Holdermann I, Beyer P, Al-Babili S
(2008) Carotenoid oxygenases involved in
plant branching catalyse a highly specific conserved apocarotenoid cleavage reaction. Biochem J 416(2):289–296.. BJ20080568.
https://doi.org/10.1042/BJ20080568
47. Sergeant MJ, Li JJ, Fox C, Brookbank N,
Rea D, Bugg TD, Thompson AJ (2009) Selective inhibition of carotenoid cleavage dioxygenases: phenotypic effects on shoot
Determination of In Vitro and In Vivo Activities of Plant Carotenoid. . .
73