FTICR, see Fourier transform ion cyclotron resonance
(FTICR)
Fungi........................................................... 3, 7–9, 16, 40,
117, 118, 145, 146, 199, 235, 293, 294,
343–345, 358
Fusarium fujikuroi..................................... 343, 345, 346,
351–352, 354, 356–358
G
Gas chromatography (GC) ............................... 65, 67–70,
166, 168, 170, 172, 173, 264
GC, see Gas chromatography (GC)
Gas chromatography-mass spectrometry
(GC-MS)............................. 64, 68, 165, 166, 168
Genome editing ...........................................321–323, 328
Geranylgeranyl diphosphate
synthase................................................................ 27–36
synthase activity ................................................... 27–36
Geranylgeranyl pyrophosphate, see Geranylgeranyl
diphosphate
GGPP, see Geranylgeranyl diphosphate
GR24 ........................................................... 202, 204, 205
H
Headspace..............................................69, 168, 170, 172
Heme ......................................................... 6, 7, 54, 57–60
High performance liquid chromatography
(HPLC)..................................................64, 65, 67,
68, 70, 77, 83, 85, 87, 96, 119, 120, 122–128,
137, 146–148, 150–154, 156, 159, 160, 179,
202, 265, 281, 283–288, 296–298, 382, 391,
394, 396, 397
HPLC, see High performance liquid chromatography
(HPLC)
HPLC-diode array detector (HPLC-DAD) ....... 117–132
HPLC-mass spectrometry (HPLC-MS) ............. 137, 381
I
Intervention studies ............................363–371, 404, 405
In vitro culture ..................................................... 315, 318
In vitro digestion .........................................363, 375–385
Isoprenoids .........................................................v, 3, 4, 27,
28, 30, 117, 263
Isotope labeling.................................................... 263, 264
Isotopologs................................................. 264, 266–268,
270, 271, 273–276
L
Liquid chromatography-mass spectrometry
(LC-MS) ...................................29, 64, 95, 97, 98,
136–138, 141, 177–185, 202, 204–205, 210,
263–275, 380–382
LC-MS, see Liquid chromatography-mass spectrometry
(LC-MS)
M
Maltose Binding Protein (MBP)....................... 54, 58–60
Mass spectrometry (MS)............................ 118, 135–143,
170, 172, 179, 180, 182–185, 205, 210–213,
215, 218, 264, 265, 267–271, 274, 294, 314,
315, 381, 382
MBP, see Maltose Binding Protein (MBP)
Metabolic syndrome ................................... 403, 408, 411
Metabolomics ......................................136, 174, 190, 196
Micellarization index............................................ 375, 378
Microscopy
bright field ............................................. 246, 249, 250
confocal........................................................... 225, 230
electron ............................................ 15, 196, 235–242
fluorescence ...................................224, 225, 229, 230
light................................................................. 245–259
Raman ............................................................. 248–259
transmission .............................................. 15, 235–242
Microsomes ...........................................91, 93, 94, 96, 97
Molar coefficient ............................ 83, 87, 286, 288, 290
MS, see Mass spectrometry (MS)
Mutation....................................................................4, 337
knockout.................................................................. 335
loss-of-function ................................................ 10, 328
null ........................................................................... 337
site-directed ............................................................. 321
targeted.................................................................... 321
Mycorrhizal quantification .................................. 295, 296
N
Neurosporaxanthin .............................178, 343, 344, 356
Nicotiana benthamiana .............................. 304, 307, 309
Norflurazon .........................................280–284, 286, 287
O
Obesity................................................................. 364, 388,
403–409, 411–413
Orbitrap ...............................................179, 180, 183, 264
Oxidative stress......................................... v, 146, 387–399
P
Photoprotection ....................................14, 117, 145, 223
Photosynthesis............................................... v, 15, 27, 40,
145, 190, 228, 263
Phytoene
desaturase........................................... 5, 6, 8, 280, 307
quantification................................. 281, 286–288, 290
synthase.................................................. 5, 8, 280, 307
synthase activity ................................................... 47–49
Phytoene synthase (PSY) ..........................4–7, 15, 39–51,
224, 231, 280, 307
Polyprotein ..........................................304–306, 308, 310
Potyvirus ............................................................... 303–311
420
PLANT AND FOOD CAROTENOIDS: METHODS AND PROTOCOLS
Index
(FTICR)
Fungi........................................................... 3, 7–9, 16, 40,
117, 118, 145, 146, 199, 235, 293, 294,
343–345, 358
Fusarium fujikuroi..................................... 343, 345, 346,
351–352, 354, 356–358
G
Gas chromatography (GC) ............................... 65, 67–70,
166, 168, 170, 172, 173, 264
GC, see Gas chromatography (GC)
Gas chromatography-mass spectrometry
(GC-MS)............................. 64, 68, 165, 166, 168
Genome editing ...........................................321–323, 328
Geranylgeranyl diphosphate
synthase................................................................ 27–36
synthase activity ................................................... 27–36
Geranylgeranyl pyrophosphate, see Geranylgeranyl
diphosphate
GGPP, see Geranylgeranyl diphosphate
GR24 ........................................................... 202, 204, 205
H
Headspace..............................................69, 168, 170, 172
Heme ......................................................... 6, 7, 54, 57–60
High performance liquid chromatography
(HPLC)..................................................64, 65, 67,
68, 70, 77, 83, 85, 87, 96, 119, 120, 122–128,
137, 146–148, 150–154, 156, 159, 160, 179,
202, 265, 281, 283–288, 296–298, 382, 391,
394, 396, 397
HPLC, see High performance liquid chromatography
(HPLC)
HPLC-diode array detector (HPLC-DAD) ....... 117–132
HPLC-mass spectrometry (HPLC-MS) ............. 137, 381
I
Intervention studies ............................363–371, 404, 405
In vitro culture ..................................................... 315, 318
In vitro digestion .........................................363, 375–385
Isoprenoids .........................................................v, 3, 4, 27,
28, 30, 117, 263
Isotope labeling.................................................... 263, 264
Isotopologs................................................. 264, 266–268,
270, 271, 273–276
L
Liquid chromatography-mass spectrometry
(LC-MS) ...................................29, 64, 95, 97, 98,
136–138, 141, 177–185, 202, 204–205, 210,
263–275, 380–382
LC-MS, see Liquid chromatography-mass spectrometry
(LC-MS)
M
Maltose Binding Protein (MBP)....................... 54, 58–60
Mass spectrometry (MS)............................ 118, 135–143,
170, 172, 179, 180, 182–185, 205, 210–213,
215, 218, 264, 265, 267–271, 274, 294, 314,
315, 381, 382
MBP, see Maltose Binding Protein (MBP)
Metabolic syndrome ................................... 403, 408, 411
Metabolomics ......................................136, 174, 190, 196
Micellarization index............................................ 375, 378
Microscopy
bright field ............................................. 246, 249, 250
confocal........................................................... 225, 230
electron ............................................ 15, 196, 235–242
fluorescence ...................................224, 225, 229, 230
light................................................................. 245–259
Raman ............................................................. 248–259
transmission .............................................. 15, 235–242
Microsomes ...........................................91, 93, 94, 96, 97
Molar coefficient ............................ 83, 87, 286, 288, 290
MS, see Mass spectrometry (MS)
Mutation....................................................................4, 337
knockout.................................................................. 335
loss-of-function ................................................ 10, 328
null ........................................................................... 337
site-directed ............................................................. 321
targeted.................................................................... 321
Mycorrhizal quantification .................................. 295, 296
N
Neurosporaxanthin .............................178, 343, 344, 356
Nicotiana benthamiana .............................. 304, 307, 309
Norflurazon .........................................280–284, 286, 287
O
Obesity................................................................. 364, 388,
403–409, 411–413
Orbitrap ...............................................179, 180, 183, 264
Oxidative stress......................................... v, 146, 387–399
P
Photoprotection ....................................14, 117, 145, 223
Photosynthesis............................................... v, 15, 27, 40,
145, 190, 228, 263
Phytoene
desaturase........................................... 5, 6, 8, 280, 307
quantification................................. 281, 286–288, 290
synthase.................................................. 5, 8, 280, 307
synthase activity ................................................... 47–49
Phytoene synthase (PSY) ..........................4–7, 15, 39–51,
224, 231, 280, 307
Polyprotein ..........................................304–306, 308, 310
Potyvirus ............................................................... 303–311
420
PLANT AND FOOD CAROTENOIDS: METHODS AND PROTOCOLS
Index