sample. For all the compounds considered in the fourth extraction
only some traces were eventually present [17]. The multiple extraction process could be compared to a traditional solid–liquid extraction method. Here, the recording of reliable quantitative data
requires the extraction to be repeated several times until sample
decoloration, thus consuming a very high amount of organic solvent and sample in a time consuming process.
Table 3
Optimized multiple reaction monitoring (MRM) with molecular ion (m/z), quantifier (Q) and qualifier
(q) transitions, and Collision Energy (CE) for apocarotenoid analysis
Compound
(+/À)
a
m/z
Quantifier
CE (V)
Qualifier
CE (V)
Apo-8
0 -Zeaxanthinal
(+)
433
433 > 119
À30
433 > 105
À35
Apo-10
0 -Zeaxanthinal
(+)
393
393 > 119
À25
393 > 105
À35
Apo-12
0 -Zeaxanthinal
(+)
367
367 > 105
À35
367 > 119
À30
Apo-14
0 -Zeaxanthinal
(+)
327
327 > 105
À35
327 > 119
À30
Apo-15
0 -Zeaxanthinal
(+)
301
301 > 173
-15
301 > 105
À30
β-Apo-8
0 -Carotenal
(+)
417
417 > 119
À25
417 > 105
À35
β-Apo-10
0 -Carotenal
(+)
377
377 > 105
À35
377 > 119
À30
β-Apo-12
0 -Carotenal
(+)
351
351 > 119
À25
351 > 105
À35
β-Apo-14
0 -Carotenal
(+)
311
311 > 105
À30
311 > 119
À25
ε-Apo-8
0 -Luteinal
(À)
415
415 > 119
40
415 > 91
50
ε-Apo-10
0 -Luteinal
(À)
375
375 > 105
40
375 > 91
50
ε-Apo-12
0 -Luteinal
(À)
349
349 > 105
40
349 > 91
50
ε-Apo-14
0 -Luteinal
(À)
309
309 > 91
50
309 > 105
40
ε-Apo-15
0 -Luteinal
(À)
283
283 > 105
40
283 > 91
50
Apo-8
0 -Capsorubinal
(+)
449
449 > 109
À25
449 > 127
À15
Apo-10
0 -Capsorubinal
(+)
409
409 > 109
À20
409 > 127
À15
Apo-12
0 -Capsorubinal
(+)
383
383 > 109
À20
383 > 127
À15
Apo-14
0 -Capsorubinal
(+)
343
343 > 109
À20
343 > 127
À15
Apo-15
0 -Capsorubinal
(+)
317
317 > 109
À20
317 > 127
À15
Apo-8
0 -Capsorubinal
(À)
448
448 > 239
25
448 > 342
20
Apo-10
0 -Capsorubinal
(À)
408
408 > 199
25
408 > 302
20
Apo-12
0 -Capsorubinal
(À)
382
382 > 276
20
382 > 173
25
Apo-14
0 -Capsorubinal
(À)
342
342 > 173
25
342 > 153
25
Apo-15
0 -Capsorubinal
(À)
316
316 > 169
20
316 > 147
20
a
(+) APCI positive; (À) APCI negative
216
Daniele Giuffrida et al.
only some traces were eventually present [17]. The multiple extraction process could be compared to a traditional solid–liquid extraction method. Here, the recording of reliable quantitative data
requires the extraction to be repeated several times until sample
decoloration, thus consuming a very high amount of organic solvent and sample in a time consuming process.
Table 3
Optimized multiple reaction monitoring (MRM) with molecular ion (m/z), quantifier (Q) and qualifier
(q) transitions, and Collision Energy (CE) for apocarotenoid analysis
Compound
(+/À)
a
m/z
Quantifier
CE (V)
Qualifier
CE (V)
Apo-8
0 -Zeaxanthinal
(+)
433
433 > 119
À30
433 > 105
À35
Apo-10
0 -Zeaxanthinal
(+)
393
393 > 119
À25
393 > 105
À35
Apo-12
0 -Zeaxanthinal
(+)
367
367 > 105
À35
367 > 119
À30
Apo-14
0 -Zeaxanthinal
(+)
327
327 > 105
À35
327 > 119
À30
Apo-15
0 -Zeaxanthinal
(+)
301
301 > 173
-15
301 > 105
À30
β-Apo-8
0 -Carotenal
(+)
417
417 > 119
À25
417 > 105
À35
β-Apo-10
0 -Carotenal
(+)
377
377 > 105
À35
377 > 119
À30
β-Apo-12
0 -Carotenal
(+)
351
351 > 119
À25
351 > 105
À35
β-Apo-14
0 -Carotenal
(+)
311
311 > 105
À30
311 > 119
À25
ε-Apo-8
0 -Luteinal
(À)
415
415 > 119
40
415 > 91
50
ε-Apo-10
0 -Luteinal
(À)
375
375 > 105
40
375 > 91
50
ε-Apo-12
0 -Luteinal
(À)
349
349 > 105
40
349 > 91
50
ε-Apo-14
0 -Luteinal
(À)
309
309 > 91
50
309 > 105
40
ε-Apo-15
0 -Luteinal
(À)
283
283 > 105
40
283 > 91
50
Apo-8
0 -Capsorubinal
(+)
449
449 > 109
À25
449 > 127
À15
Apo-10
0 -Capsorubinal
(+)
409
409 > 109
À20
409 > 127
À15
Apo-12
0 -Capsorubinal
(+)
383
383 > 109
À20
383 > 127
À15
Apo-14
0 -Capsorubinal
(+)
343
343 > 109
À20
343 > 127
À15
Apo-15
0 -Capsorubinal
(+)
317
317 > 109
À20
317 > 127
À15
Apo-8
0 -Capsorubinal
(À)
448
448 > 239
25
448 > 342
20
Apo-10
0 -Capsorubinal
(À)
408
408 > 199
25
408 > 302
20
Apo-12
0 -Capsorubinal
(À)
382
382 > 276
20
382 > 173
25
Apo-14
0 -Capsorubinal
(À)
342
342 > 173
25
342 > 153
25
Apo-15
0 -Capsorubinal
(À)
316
316 > 169
20
316 > 147
20
a
(+) APCI positive; (À) APCI negative
216
Daniele Giuffrida et al.