Fig. 3 The corresponding enlargements of the ion chromatograms obtained in SIM (APCI negative) mode,
relative to the detected apocarotenoid zone obtained by the SFE-SFC-MS analysis, of yellow tamarillo fruits
(reproduced with permission from Ref. [18]; Copyright 2018, Elsevier Inc.). List of detected apocarotenoids:
(1) Apo-8
0 -Zeaxanthinal; (2) apo-10
0 -zeaxanthinal; (3) apo-12
0 -zeaxanthinal; (4) apo-14
0 -zeaxanthinal;
(5) apo-15
0 -zeaxanthinal; (6) apo-12
0 -carotenal; (7) apo-14
0 -carotenal; (8) apo-10
0 -zeaxanthinal-c4:0;
(9) apo-8
0 -zeaxanthinal-C12:0
Table 1
SFE-SFC conditions
SFE
Flow
(mL min
À1
)
Time
(min)
CO 2 (%)
MeOH (%)
Extraction
vessel
Temperature
(
C)
Backpressure
(bar)
Static
extraction
2
3
95
5
80
150
Dynamic
extraction
2
1
90
10
80
150
SFC
Time (min) CO 2 (%)
MeOH
(%)
Flow
(mL min
À1 )
Temperature
(
C)
Backpressure
(bar)
Makeup pump
MeOH
(mL min
À1 )
0
100
0
2
100
0
2
35
150
0.5
10
60
40
12
60
40
This methodology was applied to the carotenoid and apocarotenoid analysis of lyophilized yellow tamarillo samples [18]
214
Daniele Giuffrida et al.