Retention time (min)
0
5
10
15
20
25
1
2
3
5
e
c
n
a
b
r
o
s
b
A
( 4 5 0 n m )
446
400
500
600
5
Wavelength (nm)
474
Absorbance
400
500
600
4
Wavelength (nm)
448
477
3
2
482
456
Absorbance
1
400
500
600
363
464
Wavelength (nm)
423
4
Fig. 5 Chromatogram of the HPLC separation and UV/Vis spectra of the major carotenoids present in an extract
obtained from egg-yolks from farmed hens. Detection wavelength at 450 nm. Peak identities: 1, zeaxanthin;
2, lutein; 3, canthaxanthin; 4, cis-canthaxanthin; 5, β-apo-8
0 -carotenoic acid ethyl ester
128
Da ´ maso Hornero-Me ´ ndez
0
5
10
15
20
25
1
2
3
5
e
c
n
a
b
r
o
s
b
A
( 4 5 0 n m )
446
400
500
600
5
Wavelength (nm)
474
Absorbance
400
500
600
4
Wavelength (nm)
448
477
3
2
482
456
Absorbance
1
400
500
600
363
464
Wavelength (nm)
423
4
Fig. 5 Chromatogram of the HPLC separation and UV/Vis spectra of the major carotenoids present in an extract
obtained from egg-yolks from farmed hens. Detection wavelength at 450 nm. Peak identities: 1, zeaxanthin;
2, lutein; 3, canthaxanthin; 4, cis-canthaxanthin; 5, β-apo-8
0 -carotenoic acid ethyl ester
128
Da ´ maso Hornero-Me ´ ndez