Retention time (min)
0
5
10
15
20
25
1
2
3
7
5
6
e
c
n
a
b
r
o
s
b
A
( 4 5 0 n m )
454
480
400
500
600
6
Wavelength (nm)
5
474 505
447
Absorbance
400
500
600
4
454
480
Wavelength (nm)
448
477
3
2
482
456
Absorbance
1
450
479
2’
3’
400
500
600
7
460
492
Wavelength (nm)
432
4
Absorbance
Fig. 4 Chromatogram of the HPLC separation and UV/Vis spectra of the major carotenoids present in an extract
(saponified) obtained from sarsaparilla berries (Smilax aspera L.). Detection wavelength at 450 nm. Peak
identities: 1, antheraxanthin; 2, zeaxanthin; 2
0 , cis-zeaxanthin; 3, lutein; 3
0 , cis-lutein; 4, lycophyll; 5, β-cryptoxanthin; 6, lycopene; 7, β-carotene
HPLC Analysis of Carotenoids
127
0
5
10
15
20
25
1
2
3
7
5
6
e
c
n
a
b
r
o
s
b
A
( 4 5 0 n m )
454
480
400
500
600
6
Wavelength (nm)
5
474 505
447
Absorbance
400
500
600
4
454
480
Wavelength (nm)
448
477
3
2
482
456
Absorbance
1
450
479
2’
3’
400
500
600
7
460
492
Wavelength (nm)
432
4
Absorbance
Fig. 4 Chromatogram of the HPLC separation and UV/Vis spectra of the major carotenoids present in an extract
(saponified) obtained from sarsaparilla berries (Smilax aspera L.). Detection wavelength at 450 nm. Peak
identities: 1, antheraxanthin; 2, zeaxanthin; 2
0 , cis-zeaxanthin; 3, lutein; 3
0 , cis-lutein; 4, lycophyll; 5, β-cryptoxanthin; 6, lycopene; 7, β-carotene
HPLC Analysis of Carotenoids
127