Retention time (min)
0
5
10
15
20
25
1 2
3
5
e
c
n
a
b
r
o
s
b
A
( 4 5 0 n m )
448
477
400
500
600
6
Wavelength (nm)
454
480
e
c
n
a
b
r
o
s
b
A
400
500
600
5
Wavelength (nm)
7
2
482
456
e
c
n
a
b
r
o
s
b
A
1
400
500
600
481
509
Wavelength (nm)
4
6
7
8
400
500
600
Wavelength (nm)
8
473
3
356
469
4
454
480
445 474
420
422
Fig. 3 Chromatogram of the HPLC separation and UV/Vis spectra of the major carotenoids present in an
extract (saponified) obtained from red pepper (Capsicum annuum L.) fruits. Detection wavelength at 450 nm.
Peak identities: 1, capsorubin; 2, violaxanthin; 3, capsanthin; 4, cis-capsanthin; 5, zeaxanthin; 6, cucurbitaxanthin A; 7, β-cryptoxanthin; 8, β-carotene
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Da ´ maso Hornero-Me ´ ndez