Retention time (min)
0
5
10
15
20
25
1
2
3
e
c
n
a
b
r
o
s
b
A
( 4 5 0 n m )
454
480
400
500
600
4
Wavelength (nm)
2
474
505
447
400
500
600
3
Wavelength (nm)
448
477
1
460
492
432
4
e
c
n
a
b
r
o
s
b
A
400
500
600
Wavelength (nm)
400
500
600
Wavelength (nm)
Fig. 2 Chromatogram of the HPLC separation and UV/Vis spectra of the major carotenoids present in an extract
obtained from tomato (Solanum lycopersicum L.) fruits. Detection wavelength at 450 nm. Peak identities:
1, lutein; 2, lycophyll (dihydroxy-lycopene); 3, lycopene; 4, β-carotene
HPLC Analysis of Carotenoids
125