Retention time (min)
0
5
10
15
20
25
1
2
3
4
e
c
n
a
b
r
o
s
b
A
( 4 5 0 n m )
454
480
400
500
600
4
Wavelength (nm)
3
Absorbance
400
500
600
Wavelength (nm)
2
Absorbance
1
445 474
3’
Chl b
Chl a
457
646
430
663
448
477
420
440 468
414
423
400
500
600
Wavelength (nm)
700
Chl b
Chl a
Fig. 1 Chromatogram of the HPLC separation and UV/Vis spectra of the major carotenoids present in an extract
obtained from fresh spinach (Spinacia oleracea L.) leaves. Detection wavelength at 450 nm. Peak identities:
1, (9
0
Z )-neoxanthin; 2, violaxanthin; 3, lutein; 5, β-carotene. Chlorophyll a (Chl a) and chlorophyll b (Chl b) are
also present in the chromatogram
124
Da ´ maso Hornero-Me ´ ndez
0
5
10
15
20
25
1
2
3
4
e
c
n
a
b
r
o
s
b
A
( 4 5 0 n m )
454
480
400
500
600
4
Wavelength (nm)
3
Absorbance
400
500
600
Wavelength (nm)
2
Absorbance
1
445 474
3’
Chl b
Chl a
457
646
430
663
448
477
420
440 468
414
423
400
500
600
Wavelength (nm)
700
Chl b
Chl a
Fig. 1 Chromatogram of the HPLC separation and UV/Vis spectra of the major carotenoids present in an extract
obtained from fresh spinach (Spinacia oleracea L.) leaves. Detection wavelength at 450 nm. Peak identities:
1, (9
0
Z )-neoxanthin; 2, violaxanthin; 3, lutein; 5, β-carotene. Chlorophyll a (Chl a) and chlorophyll b (Chl b) are
also present in the chromatogram
124
Da ´ maso Hornero-Me ´ ndez