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in living yeast cells. J Vis Exp 77:50633
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cells by fluorescent nanosensors. J Biol Chem 278(21):19127–19133
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Warburg effect in single cancer cells. PLoS One 8(2):e57712
153. Nagai T, Miyawaki A (2004) A high-throughput method for development of FRET-based
indicators for proteolysis. Biochem Biophys Res Commun 319(1):72–77
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activity and its response to drugs in living cells. Clin Cancer Res 16(15):3964–3975
155. Nobis M et al (2013) Intravital FLIM-FRET imaging reveals dasatinib-induced spatial control
of src in pancreatic cancer. Cancer Res 73(15):4674–4686
156. Randriamampita C et al (2008) A novel ZAP-70 dependent FRET based biosensor reveals
kinase activity at both the immunological synapse and the antisynapse. PLoS One 3(1):e1521
157. Paster W et al (2009) Genetically encoded Forster resonance energy transfer sensors for the
conformation of the Src family kinase Lck. J Immunol 182(4):2160–2167
158. Grashoff C et al (2010) Measuring mechanical tension across vinculin reveals regulation of
focal adhesion dynamics. Nature 466(7303):263–266
159. Conway DE et al (2013) Fluid shear stress on endothelial cells modulates mechanical tension
across VE-cadherin and PECAM-1. Curr Biol 23(11):1024–1030
160. Borghi N et al (2012) E-cadherin is under constitutive actomyosin-generated tension that is
increased at cell-cell contacts upon externally applied stretch. Proc Natl Acad Sci U S A 109
(31):12568–12573
161. Potzkei J et al (2012) Real-time determination of intracellular oxygen in bacteria using a
genetically encoded FRET-based biosensor. BMC Biol 10:28
162. Conway JR, Carragher NO, Timpson P (2014) Developments in preclinical cancer imaging:
innovating the discovery of therapeutics. Nat Rev Cancer 14(5):314–328
163. Bernardini A et al (2015) Type I cell ROS kinetics under hypoxia in the intact mouse carotid
body ex vivo: a FRET-based study. Am J Physiol Cell Physiol 308(1):C61–C67
164. Awaji T et al (2001) Novel green fluorescent protein-based ratiometric indicators for monitoring pH in defined intracellular microdomains. Biochem Biophys Res Commun 289
(2):457–462
165. Urra J et al (2008) A genetically encoded ratiometric sensor to measure extracellular pH in
microdomains bounded by basolateral membranes of epithelial cells. Pflugers Arch 457
(1):233–242
166. Heydorn A et al (2006) Protein translocation assays: key tools for accessing new biological
information with high-throughput microscopy. Methods Enzymol 414:513–530
167. Knauer SK et al (2005) Translocation biosensors to study signal-specific nucleo-cytoplasmic
transport, protease activity and protein-protein interactions. Traffic 6(7):594–606
168. Fetz V, Stauber RH, Knauer SK (2018) Translocation biosensors-versatile tools to probe
protein functions in living cells. Methods Mol Biol 1683:195–210
169. Dieguez-Hurtado R et al (2011) A Cre-reporter transgenic mouse expressing the far-red
fluorescent protein Katushka. Genesis 49(1):36–45
170. Yamaguchi Y et al (2011) Live imaging of apoptosis in a novel transgenic mouse highlights its
role in neural tube closure. J Cell Biol 195(6):1047–1060
171. Audet M et al (2010) Protein-protein interactions monitored in cells from transgenic mice
using bioluminescence resonance energy transfer. FASEB J 24(8):2829–2838
172. Hoffman RM (2005) The multiple uses of fluorescent proteins to visualize cancer in vivo.
Nat Rev Cancer 5(10):796–806
173. Hara M et al (2004) Imaging endoplasmic reticulum calcium with a fluorescent biosensor in
transgenic mice. Am J Physiol Cell Physiol 287(4):C932–C938
182
U. K. Sukumar et al.