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126. Forster T (1946) Energiewanderung und Fluoreszenz. Naturwissenschaften 33(6):166–175
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139. Kominami K et al (2012) In vivo imaging of hierarchical spatiotemporal activation of caspase8 during apoptosis. PLoS One 7(11):e50218
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a newly described cell death program. Biotechnol J 9(2):224–240
141. Li M et al (2012) A high-throughput FRET-based assay for determination of Atg4 activity.
Autophagy 8(3):401–412
142. Lu P et al (2011) Extracellular matrix degradation and remodeling in development and disease.
Cold Spring Harb Perspect Biol 3(12):a005058
143. Eichorst JP, Clegg RM, Wang Y (2012) Red-shifted fluorescent proteins monitor enzymatic
activity in live HT-1080 cells with fluorescence lifetime imaging microscopy (FLIM).
J Microsc 248(1):77–89
144. Hou BH et al (2011) Optical sensors for monitoring dynamic changes of intracellular metabolite levels in mammalian cells. Nat Protoc 6(11):1818–1833
145. Gavet O, Pines J (2010) Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis.
Dev Cell 18(4):533–543
146. Miura H, Matsuda M, Aoki K (2014) Development of a FRET biosensor with high specificity
for Akt. Cell Struct Funct 39(1):9–20
147. Yoshizaki H et al (2007) Akt-PDK1 complex mediates epidermal growth factor-induced
membrane protrusion through Ral activation. Mol Biol Cell 18(1):119–128
148. Seong J et al (2013) Distinct biophysical mechanisms of focal adhesion kinase
mechanoactivation by different extracellular matrix proteins. Proc Natl Acad Sci U S A 110
(48):19372–19377
Applications of Fluorescent Protein-Based Sensors in Bioimaging
181
ratio. Nat Methods 6(2):161–166
124. Hernandez-Barrera A et al (2015) Hyper, a hydrogen peroxide sensor, indicates the sensitivity
of the Arabidopsis root elongation zone to aluminum treatment. Sensors 15(1):855–867
125. Niethammer P et al (2009) A tissue-scale gradient of hydrogen peroxide mediates rapid wound
detection in zebrafish. Nature 459(7249):996–999
126. Forster T (1946) Energiewanderung und Fluoreszenz. Naturwissenschaften 33(6):166–175
127. Ma L, Yang F, Zheng J (2014) Application of fluorescence resonance energy transfer in
protein studies. J Mol Struct 1077:87–100
128. Tamura T, Hamachi I (2014) Recent progress in design of protein-based fluorescent biosensors
and their cellular applications. ACS Chem Biol 9(12):2708–2717
129. Day RN, Davidson MW (2012) Fluorescent proteins for FRET microscopy: monitoring
protein interactions in living cells. Bioessays 34(5):341–350
130. Nikolaev VO, Gambaryan S, Lohse MJ (2006) Fluorescent sensors for rapid monitoring of
intracellular cGMP. Nat Methods 3(1):23–25
131. Wallace DJ et al (2008) Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor.
Nat Methods 5(9):797–804
132. Shcherbo D et al (2009) Practical and reliable FRET/FLIM pair of fluorescent proteins. BMC
Biotechnol 9:24
133. Zhang J et al (2001) Genetically encoded reporters of protein kinase A activity reveal impact of
substrate tethering. Proc Natl Acad Sci U S A 98(26):14997–15002
134. Tsutsui H et al (2008) Improving membrane voltage measurements using FRET with new
fluorescent proteins. Nat Methods 5(8):683–685
135. Mutoh H et al (2009) Spectrally-resolved response properties of the three most advanced
FRET based fluorescent protein voltage probes. PLoS One 4(2):e4555
136. Tyas L et al (2000) Rapid caspase-3 activation during apoptosis revealed using fluorescenceresonance energy transfer. EMBO Rep 1(3):266–270
137. Wu X et al (2006) Measurement of two caspase activities simultaneously in living cells by a
novel dual FRET fluorescent indicator probe. Cytometry A 69(6):477–486
138. Bozza WP et al (2014) The use of a stably expressed FRET biosensor for determining the
potency of cancer drugs. PLoS One 9(9):e107010
139. Kominami K et al (2012) In vivo imaging of hierarchical spatiotemporal activation of caspase8 during apoptosis. PLoS One 7(11):e50218
140. Sipieter F et al (2014) Shining light on cell death processes – a novel biosensor for necroptosis,
a newly described cell death program. Biotechnol J 9(2):224–240
141. Li M et al (2012) A high-throughput FRET-based assay for determination of Atg4 activity.
Autophagy 8(3):401–412
142. Lu P et al (2011) Extracellular matrix degradation and remodeling in development and disease.
Cold Spring Harb Perspect Biol 3(12):a005058
143. Eichorst JP, Clegg RM, Wang Y (2012) Red-shifted fluorescent proteins monitor enzymatic
activity in live HT-1080 cells with fluorescence lifetime imaging microscopy (FLIM).
J Microsc 248(1):77–89
144. Hou BH et al (2011) Optical sensors for monitoring dynamic changes of intracellular metabolite levels in mammalian cells. Nat Protoc 6(11):1818–1833
145. Gavet O, Pines J (2010) Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis.
Dev Cell 18(4):533–543
146. Miura H, Matsuda M, Aoki K (2014) Development of a FRET biosensor with high specificity
for Akt. Cell Struct Funct 39(1):9–20
147. Yoshizaki H et al (2007) Akt-PDK1 complex mediates epidermal growth factor-induced
membrane protrusion through Ral activation. Mol Biol Cell 18(1):119–128
148. Seong J et al (2013) Distinct biophysical mechanisms of focal adhesion kinase
mechanoactivation by different extracellular matrix proteins. Proc Natl Acad Sci U S A 110
(48):19372–19377
Applications of Fluorescent Protein-Based Sensors in Bioimaging
181