18
injection of various dilutions (104–107 cfu∕mL) of the pathogenic E. coli cells in
phosphate-buffered saline (PBS) to the channel at a reduced flow rate of 5 μL∕ min
for 30 min. For example, Fig. 2.4c demonstrates the microelectrode array after
exposure to 107 cfu∕mL bacterial cells. Simultaneously, the impedance response
was continuously monitored during the sample flow-through process (Fig. 2.4d).
All samples generated a measurable response with respect to the control sample
within 5 min, with the highest concentration sample producing a response within
30 s; the responses saturated after 20 min. These results augured well for the
implementation of this sensor in continuous monitoring of flowing water supplies.
Fig. 2.4 Real-time binding of bacteria to AMP biosensors. (Adapted from Ref. [25])
J. H. Banoub and A. Mikhael
injection of various dilutions (104–107 cfu∕mL) of the pathogenic E. coli cells in
phosphate-buffered saline (PBS) to the channel at a reduced flow rate of 5 μL∕ min
for 30 min. For example, Fig. 2.4c demonstrates the microelectrode array after
exposure to 107 cfu∕mL bacterial cells. Simultaneously, the impedance response
was continuously monitored during the sample flow-through process (Fig. 2.4d).
All samples generated a measurable response with respect to the control sample
within 5 min, with the highest concentration sample producing a response within
30 s; the responses saturated after 20 min. These results augured well for the
implementation of this sensor in continuous monitoring of flowing water supplies.
Fig. 2.4 Real-time binding of bacteria to AMP biosensors. (Adapted from Ref. [25])
J. H. Banoub and A. Mikhael