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L. Schorr et al.
with high organic C and N loads. For instance the bacteria Janthinobacterium sp.
M-11 and Pseudomonas mohnii M-8 are cold-resistant heterotrophic-denitrification
bacteria, capable to remove nitrogen at temperatures around 2 °C under aerobic
and anaerobic conditions [120]. Furthermore, other temperature resistant nitrifying,
denitrifying microbes, equipped with all enzymes needed for surviving under harsh
soil and wastewater conditions by synthetic biology techniques offer a great potential to establish sustainable pathways for increasing the efficacy of N removal.
Promising approaches in liberating soils and wastewaters from TNF overloads may
be achievable by means of bio-engineering [31, 38, 77, 112, 118].
3.2 Application Possibilities of Synthetic Biology Techniques
In many parts of the world, blue jeans are in fashion, but just a few people know that
the blue colouring is harmful for people and environment [49]. Inhaling dye fumes
leads to lung damage among the workers in textile plants and wasterwaters strongly
contribute to pollutions in nearby waters as an increase in biochemical and chemical
oxygen demand or an impairment of photosynthesis [25]. Bacteria that produce high
amounts of the dying colour or bacteria that can be used as detectors towards textilecolour-based pollution demonstrate promising approaches to counteract textile plant
relates issues [28]. Ongoing attempts to modify microorganisms also deal with health
(e.g. vaccine design), with the preserving of environments (e.g. biosensor development to detect and remove pollutants) or with a revolutionary bio-economy inter
alia by energy saving via a more sustainable biofuel production for human welfare
[32, 60]. The community behind genetic engineering is increasing and the biggest
competition worldwide in synthetic biology is called iGEM (international Genetic
Engineering Machine) [10]. Students from all over the world are challenged to create
an idea to solve a “real-world problem” with means of synthetic biology and realise
it within one year. All teams have to send in biological constructs, which have to
be adapted to the BioBrick standard for an easy handling. The advantage of these
standardized biological parts is the possibility to arrange them in an optional order
to engineer new biological systems in the end. Therefore, the parts registry provided
by iGEM works as a large modular system accessible for all prospective teams ([54];
Fig. 4).
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