antibiotic and excised with A. rhizogenes followed by elicited with yeast with
50 mg/l, 100 mg/l, 200 mg/l concentration and MJ with 50, 100, 200 micromolar
concentration. The outcomes revealed that maximum biomass was obtained with
MS medium, Sucrose (3%) with twenty seven days of interval. Maximum flavonol,
hydroxy cinnamic acid derivative, hydroxybenzoic acid derivative, vanillin,
resveratrol and homogentisic were obtained with MJ elicitation (Fig. 1.10). Total
phenolic and flavonoid contents were higher with increased dose of both elicitors as
well as MJ (100 micromolar concentration) was showed maximum antioxidative
property. So these data confirmed the importance of MJ and yeast as elicitors on the
accumulation of polyphenols in A. scaber plant (Ghimire et al. 2019).
Fig. 1.10 Agrobacterium rhizogenes-mediated hairy root cultures in Aster scaber. a Hairy roots
induction, b Hairy root cultures in hormone-freeMS liquid medium, c PCR analysis of the rolC
gene in the transgenic root lines. DNA ladder marker Lane M, pRiKCTC2703 DNA C(+),
transgenic root lines induced by A. rhizogenes L1–L5, roots from a non-transgenic plant C(−).
Copyright permission obtained from Ghimire et al. @ 2019 Elsevier B.V
28
S. Saha and D. Pal
50 mg/l, 100 mg/l, 200 mg/l concentration and MJ with 50, 100, 200 micromolar
concentration. The outcomes revealed that maximum biomass was obtained with
MS medium, Sucrose (3%) with twenty seven days of interval. Maximum flavonol,
hydroxy cinnamic acid derivative, hydroxybenzoic acid derivative, vanillin,
resveratrol and homogentisic were obtained with MJ elicitation (Fig. 1.10). Total
phenolic and flavonoid contents were higher with increased dose of both elicitors as
well as MJ (100 micromolar concentration) was showed maximum antioxidative
property. So these data confirmed the importance of MJ and yeast as elicitors on the
accumulation of polyphenols in A. scaber plant (Ghimire et al. 2019).
Fig. 1.10 Agrobacterium rhizogenes-mediated hairy root cultures in Aster scaber. a Hairy roots
induction, b Hairy root cultures in hormone-freeMS liquid medium, c PCR analysis of the rolC
gene in the transgenic root lines. DNA ladder marker Lane M, pRiKCTC2703 DNA C(+),
transgenic root lines induced by A. rhizogenes L1–L5, roots from a non-transgenic plant C(−).
Copyright permission obtained from Ghimire et al. @ 2019 Elsevier B.V
28
S. Saha and D. Pal