14.3.3.3 NF-jB (P65) Inhibitory Activity and Antileishmanial Activity
NF-jB (p65) inhibitory activity of the isolated compounds (116, 122, 132, 155, and
209–214) was performed in HeLa cells by using rocaglamide as a positive control
(Pan et al. 2014). Except for N
4 -methyl talpinine (209; IC 50 value = 1.2 µM), the
remaining compounds were identified as inactive to the NF-jB inhibition.
Rocaglamide exhibited an IC 50 of 0.08 µM. In the antileishmanial activity
screening, N
4 -methyl-N
4 ,21-seco talpinine (210), 214, and villalstonine N
4 -oxide
Alstofonidine (189)
O
O
O
N
N
Alstofolinine B (190)
N
N
O
O
Alstolactone A (191)
N
HN
O
O
O
O
Macrogentine A (192)
N
N
O
O
O
Isoalstonoxine B (193)
Alstonoxine E (194)
O-Acetyltalpinine (195) N
4 -Methyl-19-epitalpinine (196)
7(S)-Talpinine oxindole (197)
19-Epi talcarpine (198)
Macrocarpine G (201); R
1 = R
2 = H
Macrocarpine H (202); R
1 = Me, R
2 = OMe
Macrocarpine E (199); R = Me, R' = H
Macrocarpine F (200); R = H, R' = Me
N
1
-Demethylalstonerine (203)
N
1 -demethylalstonerinal (204)
7-Hydroxy
pleiocarpamine (205)
6-Oxo
pleiocarpamine (206)
Alstoumerine oxindole (207)
Normacusine
B-2(S)-pseudoindoxyl (208)
HO
HN
NH
O
OH
HO
N
H
N
O
HO
H
H
H
H
O
N
O
N
O
N O
N
O
H
H
OH
H
H
O
N
OH
N
N
N
O
CHO
H
H
H
H
HO
N
N
O
R
HO
N
N
O
O
O
N
H
N
O
N
H
N
O
N
N
O
O
HO
N
N
O
O
HO
O
N
N
O
HO
OH
N
H
O
N
H
H
CH 2 OH
R'
R
1
R
2
Fig. 14.39 Chemical structures of various alkaloids from A. angustifolia (189–208)
500
A. T. Paul et al.